Leica TCS SP5 WLL STED

This Leica TCS SP5 confocal system is fitted for a broad range of experimental requirements. Along with a large range of fixed wavelength laser lines it now has a White-Light Laser (WLL) tunable within the range 470nm - 670nm for precise control over fluorophore excitation. Fitted with Hybrid Dectors (HyD) for enhanced sensitivity, and interchangeable Galvano and resonant scanners, this system is suitable for applications requiring both speed and/or improved signal-to-noise. Its Gated-STED module also enables colleciton of super-resolution images allowing investigators to distinguish objects and structures beyond the conventional resolution limit of light.
Microscope body:
Inverted
Motroised Stage:
Yes
Temp Control:
Full Enclosure
CO2:
Stage Insert
Software:
Las AF

 

Common Applications

Confocal: Confocal imaging is a fluorescence microscopy technique that optically sections the speciment preventing out of focus light from reaching the detector. This yields clear high contrast images, and together with the ability to acquire images sequentially at multiple positions the entire sample can be reconstructed in 3D.
Live Cell Imaging: Many biological processes are dynamic events and as such observing living cells is cruicial to unravelling and understanding the mechanisms behind them. This requires special consideration when imaging. Firstly the environmental conditions must be controllable and stable to ensure the cell remain healthy. Secondly, care needs to be taken that the act of imaging has as little impact as possible on the event being observed
STED: Stimulated Emission Depletion (STED) is a super resolution fluorescent microscope technique designed to enhance image resolution by bypassing the diffraction limit faced by tranditional microscopy. 
FRET

FRET (Fluorescence Resonance Energy Transfer) is a technique used largely to investigate molecular interactions, or with specially designed reports it can show if molecules are in an active or inactive state. It works by using two carefully selected fluorophores that when in close enough proximity and suitable orientation energy from the donor fluorophore can be transferred to the accepter. This transfer can be observed by monitoring the acceptors emission or the donors fluorescence lifetime.

Objective Lenses

MAG N.A CORRECTIONS IMMERSION W.D MISC.
20x 0.5 HC Plan Fluotar AIR 1.15 mm  
20x 0.7 HCX Plan Apo CS IMM 0.26 mm UV
40x 0.7 HCX Plan Fluotar AIR 0.4 mm  
63x 1.2 HCX Plan Apo CS WATER 0.22 mm UV
63x 1.4 HCX Plan Apo CS OIL 0.1 mm UV
100x 1.4 HCX Plan Apo OIL 0.1 mm STED

Light Sources

The microscope is equipped with multiple visible laser lines: 442nm (Diode laser), 458, 476, 488, 496 and 514 nm (from an Argon-ion laser); 561 nm (diode pumped solid state laser) and 633 nm (Helium-Neon laser). Additionally a new White Light Laser has been fitted allowing selection of any wavelength between 470nm - 670nm.
 
For continuous wave STED microscopy the microscope is connected to 592nm CW depletion Laser. Using STED technology the microscope can achieve resolution down to 60nm.

Filters

Detectors