AB SCIEX 4000 QTRAP

Quadrupole mass filters provide a robust means of sorting ions in a mass spectrometry experiment. When these mass analysers are incorporated in a triple quadrupole (QQQ) setup, which consists of three quadrupole mass filters in series, various specialised forms of quantitative and qualitative mass spectrometry can be conducted. This is achieved via the use of the first and third quadrupoles (Q1 and Q3, respectively) as mass filters, whilst the second quadrupole (Q2) is utilised as a collision cell in which precursor ions undergo collision-induced dissociation to produce fragment ions. The ABI 4000 QTrap is a hybrid QQQ/linear ion trap mass spectrometer, to which both 1D and 2D nano liquid chromatography (LC) separations can be interfaced. This instrument allows the array of scan types available in a QQQ setup to be exploited, with increased functionality obtained via linear ion trap mass analysis. The system is particularly well suited to highly sensitive, targeted quantitative analyses of, for example, metabolites and complex peptide mixtures in multiple reaction monitoring (MRM) experiments. Other scan types include precursor ion scans and constant neutral loss scans, which provide highly specific means of monitoring given classes of analytes, for example classes of post-translational modifications.

Contact People

Dr Valerie Wasinger

Dr Valerie Wasinger

Position: Senior Research Scientist
Facility: Bioanalytical Mass Spectrometry Facility
Category: Academic Staff

PT: Tuesday - Friday

Role & Interests

One of the major challenges facing protein analysis is the dynamic range of protein expression within massively complex samples. My interest is in the fractionation and identification of low abundance proteins and peptide and the identification of modified peptides and proteins in complex (1000's proteins) mixtures.

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