Liquid Chromatography / Mass Spectrometry

Proteins, Polymers and other High MW or Polar Molecules including Proteomics

LC/MS is now a mature method of analysis and it usually is coupled with electrospray ionisation (ESI) or atmospheric pressure chemical ionisation (APCI). Tandem mass spectrometry (MS/MS) is usually applied with these methods to facilitate structural characterisation of many types of compounds (including synthetic polymers) as well as to tailor a quantitative method that detects very low levels of a target analyte in highly complex mixtures. In addition to several LC/MS instruments, the BMSF is equipped with matrix assisted laser desorption/ionisation (MALDI) instruments which analyse samples deposited as solids on a plate. The BMSF is well equipped for the analysis of proteins, lipids, carbohydrates and other biopolymers. The BMSF is particularly experienced in protein identification using modern proteomic methods. In addition staff at the BMSF practice a wide range of quantitative proteomic analysis using isotopic labelling strategies to measure relative protein experession levels between samples and controls.

Searching all LC/MS instruments. Search all instruments.

Show
Sort by  Alphabetical  |  Frequently Used
results per page

AB SCIEX 4000 QTRAP

Quadrupole mass filters provide a robust means of sorting ions in a mass spectrometry experiment. When these mass analysers are incorporated in a triple quadrupole (QQQ) setup, which consists of three quadrupole mass filters in series, various specialised forms of quantitative and qualitative mass spectrometry can be conducted. This is achieved via the use of the first and third quadrupoles (Q1 and Q3, respectively) as mass filters, whilst the second quadrupole (Q2) is utilised as a collision cell in which precursor ions undergo collision-induced dissociation to produce fragment ions.

Read more

Applied Biosystems QSTAR Elite

ABI QStar Elite is a high-performance, hybrid quadrupole Time-of-Flight (TOF) mass spectrometer typically used with electrospray with greater sensitivity and intelligent dependent scan functions. The system is used for protein or peptide characterisation using nano liquid chromatography and data dependent tandem mass spectrometry. The system is currently used for iTRAQ quantification of differentially expressed proteins and these normally combine online multi-dimensional liquid chromatography.

Read more

Thermo LTQ FT

Thermo Scientific LTQ FT Ultra combines the most advanced Ion Trap and Fourier Transform Ion Cyclotron Resonance (ICR) technologies into a single instrument. The ICR cell design produces enhanced sensitivity and greater dynamic range. It is an ultra high resolution instrument for LC/MS with unprecedented analytical power and versatility. The instrument is provided with ESI, nano ESI and APCI ionization sources and CID and ECD fragmentation technologies.

Read more

Thermo LTQ Orbitrap Velos

Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Orbitrap Velos is a hybrid instrument that incorporates both Orbitrap and linear ion trap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments.

Read more

Thermo LTQ Orbitrap Velos ETD

Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Orbitrap Velos ETD is a hybrid instrument that incorporates both Orbitrap and linear ion trap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments.

Read more

Thermo LTQ Orbitrap XL

The Orbitrap Velos XL is based at lower campus and is used for non-biological samples. Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Thermo Scientific LTQ Orbitrap XL is a hybrid FTMS (Fourier Transform Mass Spectrometer) that incorporates the sensitive LTQ linear ion trap and Orbitrap mass analysers.

Read more

Thermo Orbitrap Fusion Lumos

Thermo Orbitrap Fusion Lumos

The Orbitrap Fusion Lumos includes an exclusive Tribrid design incorporating 3 different mass analysers (a quadrupole, an ion trap and an Orbitrap) enabling a large number of different experiments to be performed with a single mass spectrometer.  Software developments have also simplified the set-up of complex experiments.  Other significant technological advances including a brighter ion source and improved vacuum/pumping.  Additional enhancements of the Lumos include: greater scan speed, high mass resolving power, sensitivity and an improved ETD capability and EThcD fragm

Read more

Thermo QExactive Plus

QExactive mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements in both MS and MS/MS experiments; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The QE+ is a hybrid instrument that incorporates both quadrupole and Orbitrap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument.

Read more

Thermo TSQ Access

The TSQ Quantum is a high performance triple stage quadrupole mass spectrometer. It includes a syringe pump, a divert/inject valve, an atmospheric pressure ionisation (API) source, and the Xcalibur™ data system. You can operate the mass spectrometer in either electrospray ionisation (ESI) mode or atmospheric pressure chemical ionisation (APCI) mode. Typically, polar compounds such as amines, peptides, and proteins are best analysed by ESI, and non-polar compounds such as steroids are best analysed by APCI.

Read more

Waters/Micromass Q-TOF Ultima

The Waters Q-TOF Ultima is a quadrupole, orthogonal acceleration time-of-flight tandem mass spectrometer. It equipped with ESI and nanoESI interfaces. It has high resolution, sensitivity and mass accuracy. The Q-TOF Ultima is mainly used for LC-coupled MSMS analysis of peptides derived from gel spots and bands, accurate mass determination of biomolecules, protein post-translational modification studies, e.g. glycopeptide mapping.

Read more

results per page