Flow Cytometry

Flow Cytometry is a technology that simultaneously measures and analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. The equipment can both analyse and sort single cells based on these characteristics and researchers utilize this service for applications in immunology, cell biology, microbiology and many more.

The BRIL Flow Cytometry facility provides comprehensive and technical cell analysis and sorting services to groups within the campus and the wider Sydney research basin. The facility currently incorporates Analysis instruments; BD LSRFortessa™ (5 laser), BD FACS Canto II™ (x2) and access to three cell-sorting instruments; BD Influx™ (5 laser), BD FACS Jazz™ (2 laser) and BD FACSAria™II (upgraded). Basic information on instruments, capabilities and configurations are listed under 'Techniques' as well as their respective links to company websites containing more detailed information. Cell sorting services are provided in both FFS (fee for service) and DIY (do it yourself) systems.

If you require clarification or have queries about specific applications, please contact Chris Brownlee or David Snowden.

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BD FACS Jazz™ II

BD FACS Jazz™ II

The BD FACS Jazz™ II Cell Sorter is equipped with 2 excitation lasers (blue 488nm and red 642nm) and has a flexible detection configuration detecting up to 8 parameters (6 fluorescence channels, forward scatter and side scatter).

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BD FACSAria™ IIIu

BD FACSAria™ IIIu

The BD FACSAria™ IIIu cell sorter is equipped with five excitation lasers (violet 405nm, yellow/green 561nm, red 642nm and blue 488nm OR true-blue 445nm) and can detect up to 17 parameters (15 fluorescence channels plus forward scatter and side scatter).

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BD FACSCanto™ II

BD FACSCanto™ II

BD FACSCanto™ II is equipped with three excitation lasers wavelengths (405nm, 488nm and 640 nm). The Canto II cant detect up to 10 fluorescent parameters with 2 scatter options and is capable of analyzing samples between 100 and 12,000 cells per second. Software is FACS™ Diva generating standard FCS3.0 files for third party software analysis packages.

 

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BD Influx™

BD Influx™

The BD Influx™ cell sorter is equipped with five excitation lasers (ultraviolet 355nm, violet 405nm, blue 488nm, yellow/green 561nm and red 642nm) and has a flexible detection configuration with a number of filter set options, detecting up to 24 parameters (21 fluorescence channels, two forward scatter channels and one side scatter).

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BD LSRFortessa™ SORP

BD LSRFortessa™ SORP

 

The BD LSRFortessa™ SORP analyzing flow cytometer is a high-end, user-friendly flow cytometer with 5 excitation lasers and 24 detection parameters (matching our BD Influx’s configuration). Optimised for both biomedical and environmental research, this cytometer offers an optional High Throughput Sampling system, and small particle detection.

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BD LSRFortessa™ SORP X-20

BD LSRFortessa™ SORP X-20

The BD LSRFortessa™ SORP X-20 analyzing flow cytometer is equipped with five excitation lasers (ultraviolet 355nm, violet 405nm, blue 488nm, yellow/green 561nm and red 642nm) and is able to detect up to 20 parameters (18 fluorescence channels plus forward scatter and side scatter). This cytometer’s configuration has been designed to mirror that of our BD Influx (excluding small particle detection), enabling users to easily transfer or work-up experiments.

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BD™ High Throughput Sampler Option (HTS) - LSRFortessa

BD™ High Throughput Sampler Option (HTS) - LSRFortessa

The high throughput sampling (HTS) is attached to the LSRFortessa allowing users to analyse sample directly from a plate (12, 24, 96 and 384 wells). 

 

 

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