Dr Donald Thomas

Scientific Officer

Facility: Nuclear Magnetic Resonance Facility
Category: General/Technical Staff
Research profile: Click to view

Qualifications

Education

Ph.D. (Chemistry) The University of Western Australia, Australia

B.Sc. (Hons. Chemistry/Biochemistry) The University of Western Ontario, Canada

Professional Associations

Member of Royal Australian Chemical Institute

Member of Australian and New Zealand Magnetic Resonance Society

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Role

Help with the maintenance of the NMR spectrometer to ensure their smooth operation. Develop and implement new experimental procedures. Assist students and researchers with the acquisition and analysis of NMR data. Provide instruction in the proper use of the NMR Facilities spectrometers and the software to analyse data.

Background

During my Ph.D. I investigated multi-nuclear platinum complexes as anti-cancer agents. Specifically, their interactions with ligands found in vivo and DNA. A variety of methods were used in an attempt to understand these systems. First, [1H-15N] HSQC NMR was employed as a quantitative tool to assess the kinetic profile of reactions between platinum complexes and a variety of ligands. NOESY NMR was used to characterize the drug-DNA adducts in conjunction with molecular dynamics simulations using AMBER.

 

As a post-doc at the University of Saskatchewan I studied a variety of systems that involved the attachment of short peptides or oligonucleotides to gold surfaces. The biological molecule was terminated with a modified ferrocene molecule. These system were ultimately destined to be used as sensors in a wide array of situation, from medical diagnosis to drug detection. A wide variety of NMR and molecular modelling techniques were employed to assist in the characterization of these systems. Diffusion NMR was used to study ferrocene based dendrimers. The hydrodynamic radius was determined in order to estimate the size of subsequent generations of dendrimers. In total 6 generations of these dendrimers were synthesized and analysed using diffusion NMR methods.

Publications

Efficient functionalisation of dextran-aldehyde with catechin: potential applications in the treatment of cancer. Oliver S., Thomas D. S., Kavallaris M., Vittorio O., Boyer C.: Polymer Chemistry 2016, 7(14):2542-2552. DOI: 10.1039/C6PY00228E

The dual-role of Pt(IV) complexes as active drug and crosslinker for micelles based on β-cyclodextrin grafted polymer. Callari M., Thomas D. S., Stenzel M. H.: Journal of Materials Chemistry B 2016, 4(12):2114-2123. DOI: 10.1039/C5TB02429C

Silent information regulator 1 modulator resveratrol increases brain lactate production and inhibits mitochondrial metabolism, whereas SRT1720 increases oxidative metabolism. Rowlands B. D., Lau C. L., Ryall J. G., Thomas D. S., Klugmann M., Beart P. M., Rae C. D.: Journal of Neuroscience Research 2015, 93(7):1147-1156. DOI: 10.1002/jnr.23570

Competitive formation of DNA linkage isomers by a trinuclear platinum complex and the influence of pre-association. Moniodis J. J., Thomas D. S., Davies M. S., Berners-Price S. J., Farrell N. P.: Dalton Transactions 2015, 44(8):3583-3593. DOI: 10.1039/C4DT02942A

Putting corannulene in its place. Reactivity studies comparing corannulene with other aromatic hydrocarbons. George S. R. D., Frith T. D. H., Thomas D. S., Harper J. B.: Organic & Biomolecular Chemistry 2015, 13(34):9035-9041. DOI: 10.1039/C5OB01215E

The solution structure of bis(phenazine-1-carboxamide)-DNA complexes: MLN 944 binding corrected and extended. Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: Biopolymers 2014, 101(11):1099-1113. DOI: 10.1002/bip.22513

Stereoselective fluorination alters the geometry of a cyclic peptide: Exploration of backbone-fluorinated analogues of Unguisin A. Hu X.-G., Thomas D. S., Griffith R., Hunter L.: Angewandte Chemie, International Edition in English 2014, 53(24):6176-6179. DOI: 10.1002/anie.201403071

NMR spectroscopy to follow reaction progress in ionic liquids. Butler B. J., Thomas D. S., Hook J. M., Harper J. B.: Magnetic Resonance in Chemistry 2014. DOI:

Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: The Solution Structure of the MLN 944-d(TACGCGTA)2 complex. In.: Nucleic Acid Database; 2013.

Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: The Solution Structure of the MLN 944-d(TATGCATA)2 Complex. In.: Nucleic Acid Database; 2013.

Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: The Solution Structure of the MLN 944-d(ATGCAT)2 Complex. In.: Nucleic Acid Database; 2013.

Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: 4BZU: The Solution Structure of the MLN 944-d(TATGCATA)2 Complex. In.: Worldwide Protein Data Bank; 2013.

Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: 4BZT: The Solution Structure of the MLN 944-d(ATGCAT)2 Complex. In.: Worldwide Protein Data Bank; 2013.

Serobian A., Thomas D. S., Ball G. E., Denny W. A., Wakelin L. P. G.: 4BZV: The Solution Structure of the MLN 944-d(TACGCGTA)2 complex. In.: Worldwide Protein Data Bank; 2013.

An investigation into the supramolecular structure, solubility, stability and antioxidant activity of rutin/cyclodextrin inclusion complex. Nguyen T. A., Liu B., Zhao J., Thomas D. S., Hook J. M.: Food Chemistry 2013, 136(1):186-192. DOI: 10.1016/j.foodchem.2012.07.104

Using Supramolecular Binding Motifs To Provide Precise Control over the Ratio and Distribution of Species in Multiple Component Films Grafted on Surfaces: Demonstration Using Electrochemical Assembly from Aryl Diazonium Salts. Gui A. L., Yau H. M., Thomas D. S., Chockalingam M., Harper J. B., Gooding J. J.: Langmuir 2013, 29(15):4772-4781. DOI: 10.1021/la400358e

Macromolecular ruthenium complexes as anti-cancer agents. Blunden B. M., Thomas D. S., Stenzel M. H.: Polymer Chemistry 2012, 3(10):2964-2975. DOI: 10.1039/c2py20439h

Analysis of Thiol-sensitive Core-cross-linked Polymeric Micelles Carrying Nucleoside Pendant Groups using On-line' Methods: Effect of Hydrophobicity on Cross-linking and Degradation. Blunden B. M., Thomas D. S., Stenzel M. H.: Australian Journal of Chemistry 2011, 64(6):766-778. DOI: 10.1071/CH10448

Determination of the Kinetic Profile of a Dinuclear Platinum Anticancer Complex in the Presence of Sulfate: Introducing a New Tool for the Expedited Analysis of 2D [1H, 15N] HSQC NMR Spectra. Ruhayel R. A., Corry B., Braun C., Thomas D. S., Berners-Price S. J., Farrell N. P.: Inorganic Chemistry 2010, 49(23):10815-10819. DOI: 10.1021/ic100576k

Dynamics of water in agar gels studied using low and high resolution 1H NMR spectroscopy. Davies E., Huang Y., Harper J. B., Hook J. M., Thomas D. S., Burgar I. M., Lillford P. J.: International Journal of Food Science and Technology 2010, 45(12):2502-2507. DOI: 10.1111/j.1365-2621.2010.02448.x

[1H,15N] NMR studies of the aquation of cis-diamine platinum(II) complexes. Cubo L., Thomas D. S., Zhang J., Quiroga A. G., Navarro-Ranninger C., Berners-Price S. J.: Inorganica Chimica Acta 2009, 362(3):1022-1026. DOI: 10.1016/j.ica.2008.03.117

Influence of amine ligands on the aquation and cytotoxicity of trans-diamine platinum(ii) anticancer complexes. Cubo L., Quiroga A. G., Zhang J., Thomas D. S., Carnero A., Navarro-Ranninger C., Berners-Price S. J.: Dalton Transactions 2009(18):3457-3466. DOI: 10.1039/b819301k

Effects of geometric isomerism and anions on the kinetics and mechanism of the stepwise formation of long-range DNA interstrand cross-links by dinuclear platinum antitumor complexes. Zhang J., Thomas D. S., Berners-Price S. J., Farrell N.: Chemistry–A European Journal 2008, 14(21):6391-6405. DOI: 10.1002/chem.200800408

Thomas D. S.: Molecular Modelling and NMR Studies of Multinuclear Platinum Anticancer Complexes: University of Western Australia; 2006.

Reactions of 1-amino-n'-ferrocenemethylcarboxylate with electrophiles: A combined synthetic, electrochemical, and theoretical study. Khan M. A. K., Thomas D. S., Kraatz H.-B.: Inorganica Chimica Acta 2006, 359(10):3339-3344. DOI: 10.1016/j.ica.2006.04.038

Chirality and Encapsulation Properties of Disubstituted Ferrocene-Peptide Dendrimers. Appoh F. E., Thomas D. S., Kraatz H.-B.: Macromolecules 2006, 39(17):5629-5638. DOI: 10.1021/ma060525x

Effects of geometric isomerism in dinuclear platinum antitumor complexes on aquation reactions in the presence of perchlorate, acetate and phosphate. Zhang J., Thomas D. S., Davies M. S., Berners-Price S. J., Farrell N.: Journal of Biological Inorganic Chemistry 2005, 10(6):652-666. DOI: 10.1007/s00775-005-0013-5

Glutamic Acid Dendrimers Attached to a Central Ferrocene Core: Synthesis and Properties. Appoh F. E., Thomas D. S., Kraatz H.-B.: Macromolecules 2005, 38(18):7562-7570. DOI: 10.1021/ma050531d

Long Range 1,4 and 1,6-Interstrand Cross-Links Formed by a Trinuclear Platinum Complex. Minor Groove Preassociation Affects Kinetics and Mechanism of Cross-Link Formation as Well as Adduct Structure. Hegmans A., Berners-Price S. J., Davies M. S., Thomas D. S., Humphreys A. S., Farrell N.: Journal of the American Ceramic Society 2004, 126(7):2166-2180. DOI: 10.1021/ja036105u

An examination of the aquation kinetics and equilibria of dinuclear platinum anticancer agents. Zhang J., Thomas D. S., Davies M. S., Berners-Price S. J., Farrell N.: Journal of Inorganic Biochemistry 2003, 96(1):261. DOI:

NMR studies probing the directionality of DNA interstrand cross-linking by the trinuclear platinum complex BBR3464. Thomas D. S., Davies M. S., Zhang J., Berners-Price S. J., Farrell N.: Journal of Inorganic Biochemistry 2003, 1(96):239. DOI:

Competitive reactions of interstrand and intrastrand DNA‐Pt adducts: a dinuclear‐platinum complex preferentially forms a 1, 4‐interstrand cross‐link rather than a 1, 2 intrastrand cross‐link on binding to a GG 14‐mer duplex. Berners‐Price S. J., Davies M. S., Cox J. W., Thomas D. S., Farrell N.: Chemistry--A European Journal 2003, 9(3):713-725. DOI:

Competitive reactions of interstrand and intrastrand DNA-Pt adducts: A dinuclear-platinum complex preferentially forms a 1,4-interstrand cross-link rather than a 1,2 intrastrand cross-link on binding to a GG 14-mer duplex. Berners-Price S. J., Davies M. S., Cox J. W., Thomas D. S., Farrell N.: Chemistry--A European Journal 2003, 9(3):713-725. DOI: 10.1002/chem.200390080

Kinetic and Equilibria Studies of the Aquation of the Trinuclear Platinum Phase II Anticancer Agent [{trans-PtCl(NH3)2}2{μ-trans-Pt(NH3)2(NH2(CH2)6NH2)2}]4+ (BBR3464). Davies M. S., Thomas D. S., Hegmans A., Berners-Price S. J., Farrell N.: Inorganic Chemistry 2002, 41(5):1101-1109. DOI: 10.1021/ic010851n

Kinetic and equilibria studies of the aquation of the trinuclear platinum anticancer agent BBR3464. Thomas D. S., Davies M. S., Hegmans A., Berners-Price S., Farrell N.: Journal of Inorganic Biochemistry 2001, 86(1):454-454. DOI:

Preferential formation of a 1,4-interstrand DNA crosslink rather than a 1,2-intrastrand crosslink by a dinuclear platinum complex. Davies M. S., Berners-Price S. J., Cox J. W., Thomas D. S., Farrell N.: Journal of Inorganic Biochemistry 2001, 86(1):196-196. DOI:

NMR studies probing the origin of the unique antitumour activity of the new trinuclear platinum anticancer drug BBR3464. Berners-Price S. J., Davies M. S., Thomas D. S., Hegmans A., Cox J. W., Farrell N.: Journal of Inorganic Biochemistry 2001, 86(1):23-23. DOI:

Circular dichroism, kinetic and mass spectrometric studies of copper (I) and mercury (II) binding to metallothionein. Stillman M. J., Thomas D., Trevithick C., Guo X., Siu M.: Journal of Inorganic Biochemistry 2000, 79(1):11-19. DOI:

 

 

Contact

+61-2-93854706
+61-2-93854699