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FEI Nova NanoSEM 230 FE-SEM

FEI Nova NanoSEM 230 FE-SEM

The NanoSEM 230 is a field-emission scanning electron microscope (FE-SEM), which attains ultra-high imaging resolution without the specimen size restrictions of a conventional in-lens FE-SEM due to the advanced design of the electron optics. The NanoSEM 450’s Schottky field-emission source allows the user to achieve high imaging resolution at a range of kV, at both low (high-resolution imaging) and high (microanalytical imaging) currents.

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FEI Nova NanoSEM 450 FE-SEM

The NanoSEM 450 is a field-emission scanning electron microscope (FE-SEM), which attains ultra-high imaging resolution without the specimen size restrictions of a conventional in-lens FE-SEM due to the advanced design of the electron optics. The NanoSEM 450’s Schottky field-emission source allows the user to achieve high imaging resolution at a range of kV, at both low (high-resolution imaging) and high (microanalytical imaging) currents.

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FEI Quanta 200 ESEM

FEI Quanta 200 ESEM

The FEI Quanta 200 is a particularly versatile tungsten-gun scanning electron microscope capable of operating in three different vacuum modes. It is most frequently used in a high vacuum (conventional) mode to examine dry conductive specimens. Alternatively, it may be used in a low vacuum (0.1-1.0 Torr) mode to examine dry specimens that are not inherently conductive and will not tolerate normal coating procedures. It is also possible, to operate in a “wet” or Environmental (1.0 – 20 Torr) mode. In this mode, controlling the interaction between the temperature of the cold stage with the pressure within the specimen chamber, enables humidity to be maintained around the specimen during imaging. There are several imaging limitations imposed when the microscope is operated in “wet mode” therefore a research proposal (detailing the desired operating conditions) should be submitted for discussion prior to seeking ‘wet mode’ imaging. For enquiries regarding SEM access and training, please contact Simon Hager.

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FEI Tecnai G2 20 TEM

FEI Tecnai G2 20 TEM

The FEI Tecnai G2 20 TEM allows high resolution images to be obtained from thin (electron transparent) materials. The Tecnai TEM has a thermionic source (LaB6) and may be easily operated at different accelerating voltages. This makes it suitable for a wide range of specimens. The Tecnai TEM allows structural, crystallographic and elemental studies of materials. This microscope has a Bruker QUANTAX energy dispersive x-ray spectroscopy system interfaced to it and a BM Eagle digital camera for image acquisition. In addition, the microscope has a High Angle Annular Dark Field (HAADF) detector.

For further information about the use of the FEI Tecnai G2 please contact Qiang ZhuRhiannon Kuchel, or Sean Lim

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Flow Cytometry

Flow Cytometry

Flow Cytometry is a technology that simultaneously measures and analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence.

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Fluoview FV1000

Fluoview FV1000

The olympus Fluoview FV1000 is a fluorescence confocal microscope. Unlike most confocal systems it is equipped with two scan heads allowing simultaneous imaging with photo-Activation/Bleaching. This makes it ideally suited for applications such as photoconversion, Fluorescence Recovery After Photobleaching (FRAP) and uncaging experiments.

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FluoView FV1200

FluoView FV1200

The FV1200 is a filter based upright confocal system. A workhorse of our confocal systems it is well equipped for standard fixed samples where high resolution and sensitivity are required for localization based studies. Additionally as one of the few upright confocal systems within the BMIF it provides a means for visualizing water submerged opaque samples.

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Focused Ion Beam xP200

The FEI XP200 focused ion beam miller uses a fine, energetic beam of gallium ions that scan over the surface of a specimen. At high beam currents the gallium beam rapidly sputters away the specimen surface that allows subsurface cross-sections to be prepared. If the beam current is reduced, the secondary electrons or secondary ions emitted from the specimen surface, can be detected and used to form high resolution images.

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Gas Chromatography

Gas Chromatography

Chromatography is the separation of a mixture of compounds (solutes) into separate components. By separating the sample into individual components, it is easier to identify (qualitative) and measure the amount (quantitate) of the various sample components. There are numerous chromatographic techniques and corresponding instruments. Gas chromatography (GC) is one of these techniques. It is estimated that 10-20% of the known compounds can be analysed by GC.

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Gas Chromatography / Mass Spectrometry

Low Molecular Weight: Analysis of Small Molecules including Metabolomic Analysis

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GE and BioRad mini and large format SDS-PAGE systems

Proteomic work can involve the use of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE or 2-DE). In 2-DE, "first dimension" electrophoresis in a polyacrylamide gel with a pH gradient and high urea concentration is followed by a "second dimension" separation in an SDS-PAGE gel. SDS-PAGE can also be used independently. Proteins and peptides are separated within the gel according to the % acrylamide and crosslinker, given as %T and %C.

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GE Healthcare iTC200 Isothermal Calorimeter

Investigating molecular interactions, reaction kinetics and thermodynamics by Isothermal Calorimeter (iTC). Production or absorption of heat is a universal event consequent to the binding of two molecules or a chemical reaction. iTC is the only equipment that can directly measure the energetics of biochemical processes involving interaction between two molecules such as a receptor and a ligand.

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GE IPGphor/Multiphor Isoelectric Focusing Systems

Isoelectric focusing (IEF) systems are used for: the fractionation of complex biological samples in pathway and systems analysis; quantitation of protein effector molecules; elucidation of protein modifications; and sample clean-up for ongoing analysis. It is used in conjunction with immobilised pH gradients of length 7cm, 13cm, or 18cm for broad range pH3-10, mid range pH4-7, 6-11, or narrow range with 0.01pH unit separating power.

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Hitachi S3400

Hitachi S3400

EMU has two Hitachi S3400 SEMs – both are able to do imaging and X-ray microanalysis. These instruments are fitted with secondary and backscatter electron detectors that allow for topographic and compositional (atomic number contrast) surface imaging of samples. These microscopes are routinely used for imaging from ~20x to ~20,000x magnification. The S3400 SEMs are predominantly used in high-vacuum mode, but also have a variable-pressure or “natural” mode that can be useful when imaging vacuum-sensitive specimens or specimens with conductivity problems.

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In vivo Imaging

In vivo Imaging

BRIL imaging technologies encompasses current major in vivo imaging modalities such as high frequency ultrasound, positron emission tomography (PET), micro computed tomography (microCT), and optical imaging (bioluminescence and fluorescence). The data from imaging platforms such as microPET-CT and ultrasound can have maximal clinical relevance, allowing quick bench-to-bedside technology transfer.

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IncuCyte FLR

IncuCyte FLR

The IncuCyte FLR is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible, and also comes with a selection of versatile inserts allowing you to imagine your cells in a range of vessels.

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Inveon MicroPET-CT

Inveon MicroPET-CT

The Inveon μCT uses multiple axial x-rays of the animal to generate cross-sectional information or 3-dimensional reconstructions of the animal or parts of the animal. The μPET uses an LSO detector for fast scintillation decay time, high light output and effective atomic number.

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IVIS Lumina and IVIS SpectrumCT

IVIS Lumina and IVIS SpectrumCT

The IVIS Lumina provides a highly sensitive means to image fluorescent and/or bioluminescent reporters in vivo and in vitro. The IVIS SpectrumCT expands upon optical imaging capabilities as well as providing a microCT capability for anatomical referencing.

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JEOL 7001F FE-SEM

The JEOL 7001F is a Schottky field emission scanning electron microscope. The Schottky field emission electron gun produces a very finely focused, high current electron beam suitable for specialist high resolution microstructural analysis of inorganic materials. The "below lens" configured 7001F can accommodate specimens of up to 25mm in diameter, but specimen tilt is restricted because of the presence of a number of specialist microanalytical detectors within the specimen chamber.

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JEOL JSPM 5400 MkII Environmental Atomic Force Microscope

JEOL JSPM 5400 MkII is an environmental Scanning Probe Microscope which is configured for observation and nano-characterisation of inorganic and device materials. The JEOL JSPM 5400 MkII eAFM has a number of piezo-scanners and a range of atomic force imaging modes which may be operated in ambient and high vacuum conditions (~10-6 Pa) enabling control of environmental conditions (pressure and temperature) during imaging. Some examples of the imaging modes available include contact, intermittent contact and non-contact topography imaging.

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JEOL JXA-8500F Field-Emission SEM-EPMA Hyperprobe (WDS & SDD-EDS)

JEOL JXA-8500F Field-Emission SEM-EPMA Hyperprobe (WDS & SDD-EDS)

The JEOL JXA-8500F is a powerful microanalytical instrument that provides area-specific quantitative elemental results down to the sub-micron level. The electron probe microanalyser (EPMA) is fitted with four wavelength dispersive spectrometers (WDS) and a JEOL silicon drift detector energy dispersive spectrometer (SDD-EDS), giving this instrument the capability to detect and measure the concentration of most elements in the periodic table Z=4), with detection limits often better than <0.05%. In addition to its conventional microprobe capabilities, the instrument’s Schottky field-emission gun allows non-conventional low-kV work to be undertaken while maintaining a stable, highly-focused, high current (up to >100nA) beam.

Quantitative analysis may be carried out on the bulk matrix of micro-sampled materials as well as on phases, inclusions, grain boundaries or precipitates in a matrix. Examples of materials analysed using the JXA-8500F include geological specimens, fuel cells, implantable bionics, archaeological artefacts, metals/alloys and glasses.

Sample holders are available that accommodate 26mm (1") diameter resin-mounted specimens and petrographic thin sections/wafers. Samples need to be dry, flat, conductive and vacuum-stable for EPMA analysis. Usually, small pieces of an item are embedded in resin or mounted on a glass slide, polished to a mirror finish and then coated with a layer of carbon for conductivity.

This instrument was funded through the NCRIS program and is available to researchers nationwide via the AMMRF network (www.ammrf.org.au). For more information about instrument capabilities, access, and for specimen preparation advice, please contact Karen Privat.

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JEOL TEM-1400

JEOL TEM-1400

The JEOL 1400 transmission electron microscope operates at accelerating voltages up to 120kV. The microscope is equipped with a high contrast lens configuration especially suited to imaging biological, medical or polymer specimens that lack electron density and inherent contrast. Specimens undergo preparation procedures to ensure they are 100 nm or thinner in order to transmit the electron beam. Dense regions within the specimen cause electrons to scatter and an aperture, positioned near the specimen in the electron column, prevents the scattered electrons from reaching the imaging plane.

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