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Empyrean 2 (PANalytical) X-ray Diffraction System (Cobalt)

The Empyrean 2 is a Bragg-Brentano geometry X-ray diffractometer that was installed in 2014 and is equipped with the following components:

  • Cobalt anode X-ray tube to minimise fluorescence from iron-containing samples that occurs when using the standard copper X-ray anode
  • High resolution optic for cobalt X-rays to minimise scatter at low angles
  • Sample changer for large batches 

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Empyrean 3 (PANalytical) XRD with Ag source and PSD

The Empyrean 3 is fitted with a silver source and a position-sensitive detector to instantly capture a wide 2-theta angular range during an in situ XRD experiment without the need to scan the detector. The instrument can run capillary samples and the high energy silver source also enables the instrument to perform the pair distribution function (PDF) experiment that describes the distribution of distances between pairs of atoms within a material.

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EMU Specimen Preparation Equipment

For training in the use of any of the specimen preparation equipment please speak to EMU staff.

Materials Specimen Preparation

·       Gatan PIPS II Model 695

·       Hitachi IM4000 Ion Milling System

·       Buehler IsoMet 1000 Precision Saw

·       Struers Secotom-10 Saw

·       Struers LaboPol 5

·       Ultrasonics Ultrasonicator

·       Struers Tenupol-3 Electropolishing Unit

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Faxitron MX-20

Faxitron MX-20

The Faxitron MX-20 is a self contained bench-top x-ray cabinet which produces digital 2D radiographs in either png or jpg file formats. It has 5 magnification levels and laser guide for sample alignment.

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FEI Nova Nanolab 200

FEI Nova Nanolab 200

The FEI xT Nova NanoLab 200 combines a high resolution focussed ion beam (FIB) and a high resolution field emission scanning electron microscope (FESEM). It was installed in 2004 and is a flagship instrument of Australian Microscopy and Microanalysis Research Facility (AMMRF).  It was upgraded to PIA mode in 2011 for higher precision of ion milling on a new operating system.

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FEI Nova NanoSEM 230 FE-SEM

FEI Nova NanoSEM 230 FE-SEM

The NanoSEM 230 is a field-emission scanning electron microscope (FE-SEM), which attains ultra-high imaging resolution without the specimen size restrictions of a conventional in-lens FE-SEM due to the advanced design of the electron optics. The NanoSEM 450’s Schottky field-emission source allows the user to achieve high imaging resolution at a range of kV, at both low (high-resolution imaging) and high (microanalytical imaging) currents.

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FEI Nova NanoSEM 450 FE-SEM

The NanoSEM 450 is a field-emission scanning electron microscope (FE-SEM), which attains ultra-high imaging resolution without the specimen size restrictions of a conventional in-lens FE-SEM due to the advanced design of the electron optics. The NanoSEM 450’s Schottky field-emission source allows the user to achieve high imaging resolution at a range of kV, at both low (high-resolution imaging) and high (microanalytical imaging) currents.

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FEI Quanta 200 ESEM

FEI Quanta 200 ESEM

The FEI Quanta 200 is a particularly versatile tungsten-gun scanning electron microscope capable of operating in three different vacuum modes. It is most frequently used in a high vacuum (conventional) mode to examine dry conductive specimens. Alternatively, it may be used in a low vacuum (0.1-1.0 Torr) mode to examine dry specimens that are not inherently conductive and will not tolerate normal coating procedures. It is also possible, to operate in a “wet” or Environmental (1.0 – 20 Torr) mode.

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FEI Tecnai G2 20 TEM

FEI Tecnai G2 20 TEM

The FEI Tecnai G2 20 TEM allows high resolution images to be obtained from thin (electron transparent) materials. The Tecnai TEM has a thermionic source (LaB6) and may be easily operated at different accelerating voltages. This makes it suitable for a wide range of specimens. The Tecnai TEM allows structural, crystallographic and elemental studies of materials.

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Flow Cytometry

Flow Cytometry

Flow Cytometry is a technology that simultaneously measures and analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence.

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FluoView FV1200

FluoView FV1200

The FV1200 is a filter based upright confocal system. A workhorse of our confocal systems it is well equipped for standard fixed samples where high resolution and sensitivity are required for localization based studies. Additionally as one of the few upright confocal systems within the BMIF it provides a means for visualizing water submerged opaque samples.

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Focused Ion Beam xP200

The FEI XP200 focused ion beam miller uses a fine, energetic beam of gallium ions that scan over the surface of a specimen. At high beam currents the gallium beam rapidly sputters away the specimen surface that allows subsurface cross-sections to be prepared. If the beam current is reduced, the secondary electrons or secondary ions emitted from the specimen surface, can be detected and used to form high resolution images.

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Gas Chromatography

Gas Chromatography

Chromatography is the separation of a mixture of compounds (solutes) into separate components. By separating the sample into individual components, it is easier to identify (qualitative) and measure the amount (quantitate) of the various sample components. There are numerous chromatographic techniques and corresponding instruments. Gas chromatography (GC) is one of these techniques. It is estimated that 10-20% of the known compounds can be analysed by GC.

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Gas Chromatography / Mass Spectrometry

Low Molecular Weight: Analysis of Small Molecules including Metabolomic Analysis

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GE and BioRad mini and large format SDS-PAGE systems

Proteomic work can involve the use of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE or 2-DE). In 2-DE, "first dimension" electrophoresis in a polyacrylamide gel with a pH gradient and high urea concentration is followed by a "second dimension" separation in an SDS-PAGE gel. SDS-PAGE can also be used independently. Proteins and peptides are separated within the gel according to the % acrylamide and crosslinker, given as %T and %C.

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GE Healthcare iTC200 Isothermal Calorimeter

Investigating molecular interactions, reaction kinetics and thermodynamics by Isothermal Calorimeter (iTC). Production or absorption of heat is a universal event consequent to the binding of two molecules or a chemical reaction. iTC is the only equipment that can directly measure the energetics of biochemical processes involving interaction between two molecules such as a receptor and a ligand.

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GE IPGphor/Multiphor Isoelectric Focusing Systems

Isoelectric focusing (IEF) systems are used for: the fractionation of complex biological samples in pathway and systems analysis; quantitation of protein effector molecules; elucidation of protein modifications; and sample clean-up for ongoing analysis. It is used in conjunction with immobilised pH gradients of length 7cm, 13cm, or 18cm for broad range pH3-10, mid range pH4-7, 6-11, or narrow range with 0.01pH unit separating power.

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Hitachi S3400

Hitachi S3400

EMU has two Hitachi S3400 SEMs – both are able to do imaging and X-ray microanalysis. These instruments are fitted with secondary and backscatter electron detectors that allow for topographic and compositional (atomic number contrast) surface imaging of samples. These microscopes are routinely used for imaging from ~20x to ~20,000x magnification. The S3400 SEMs are predominantly used in high-vacuum mode, but also have a variable-pressure or “natural” mode that can be useful when imaging vacuum-sensitive specimens or specimens with conductivity problems.

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In vivo Imaging

In vivo Imaging

BRIL imaging technologies encompasses current major in vivo imaging modalities such as high frequency ultrasound, positron emission tomography (PET), micro computed tomography (microCT), and optical imaging (bioluminescence and fluorescence). The data from imaging platforms such as microPET-CT and ultrasound can have maximal clinical relevance, allowing quick bench-to-bedside technology transfer.

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IncuCyte FLR

IncuCyte FLR

The IncuCyte FLR is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible, and also comes with a selection of versatile inserts allowing you to imagine your cells in a range of vessels.

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Inveon MicroPET-CT

Inveon MicroPET-CT

The Inveon μCT uses multiple axial x-rays of the animal to generate cross-sectional information or 3-dimensional reconstructions of the animal or parts of the animal. The μPET uses an LSO detector for fast scintillation decay time, high light output and effective atomic number.

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IVIS Lumina and IVIS SpectrumCT

IVIS Lumina and IVIS SpectrumCT

The IVIS Lumina provides a highly sensitive means to image fluorescent and/or bioluminescent reporters in vivo and in vitro. The IVIS SpectrumCT expands upon optical imaging capabilities as well as providing a microCT capability for anatomical referencing.

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