This Leica TCS SP5 confocal system is equipped for a broad range of experimental requirements. Along with a range of fixed wavelength laser lines it now has a White-Light Laser (WLL) tunable within the range x-x for precise control over fluorophore excitation. Fitted with Hybrid Dectors (HyD) for enhanced sensitivity, and interchangeable Galvano and resonant scanners, this system is suitable for applications requiring both speed and/or improved signal-to-noise. Its Gated-STED module also enables collection of super-resolution images allowing investigators to distinguish objects and structures beyond the conventional resolution limit of light.
The Zeiss LSM 880 is an inverted laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector and 2 multialkali PMTs, which can be used as single point detectors or combined for 34 channel spectral imaging. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (140nm axially, 400 nm laterally) and 4-8 x greater signal-to-noise ratio, at the highest speed of any linear scanning confocal system. BiG.2 Type A (2x GaAsP detectors) non-descanned detectors for the multi-photon laser can be used for imaging thick specimens, with deeper penetration and decreased phototoxicity compared to single photon lasers. The Picoquant system utilises the multi-photon laser to perform Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Forster Resonance Energy Transfer (FRET). The Zeiss 880 is also equipped with polarisers for anisotropy measurements.
The Zeiss LSM 800 is an inverted Zeiss Axio Observer Z1 laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector, 2 multi-alkali PMTs and DIC optics for trans-illumination microscopy with each objective. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (120 nm in x / y and 350 nm in z) and 4-8 x better signal-to-noise ratio, with fast linear scanning. Airyscan works with thicker samples such as fixed tissue sections or whole animal mounts that need a higher penetration depth.
The Zeiss LSM 780 is a high-sensitivity laser scanning confocal microscope. Unlike most standard confocal systems this instrument comes equipped with a GaAsP photodetector. This detector in addition to boasting a quantum efficiency of 45% (in comparison to 25% for standard PMTs), it can also be used as a single-point detector or as a 32-channel detector array facilitating spectral measurements. It can also be switched into a single-photon counting mode providing great signal to noise for such techniques as FCS and RICS.
Lighsheet microscopy is a fluorescence-based imaging technique that is growing in popularity. It outperforms other techniques in its ability to rapidly capture large optically sectioned three dimensional datasets, whilst keeping photo-toxicity and bleaching to a minimum. It therefore lends itself well to imaging samples larger than those typically attempted by conventional fluorescence microscopy such as tissues, whole organs and even embryo development.
The Zeiss Elyra Superresolution Microscope is a developmental superresolution microscope with capabilities to push imaging past the classic diffraction limit. Typically visible light microscopes cannot achieve spatial resolution of greater than 200-250 nanometers. This system has two different imaging modalities to push this limit as low as 20 nanometers.
TIRFSuper-resolutionSIMFunded by Ramaciotti Foundation
The Zeiss Elyra 7 offers several super-resolution modalities, allowing detailed visualisation of samples beyond the diffraction limit of conventional light microscopy. With 2 fast CMOS cameras, this system allows users to rapidly image multiple colours. The Elyra 7 features Lattice SIM (Structured Illumination Microscopy), allowing fast and gentle super-resolution imaging (~120 nm in xy and ~300 nm in z) in 3 dimensions. The speed of this imaging modality is well suited to capturing dynamic biological processes in live samples. In addition, the Elyra 7 features TIRF imaging for improved signal to noise, and single-molecule localisation imaging for STORM, PALM and PAINT experiments (~20-30 nm xy and ~50-80 nm in z) on fixed samples.
The Zeiss Celldiscoverer 7 is a boxed imaging system suited for performing automated experiments. This inverted widefield system features automatic focusing, focus stabilisation, time-course and position setup to allow researchers insert a sample and set up long-term experiments in minutes. The Celldiscoverer 7 has a fully temperature-controlled sample chamber with a CO2 pump, this chamber is highly stable and allows for live cells to stay healthier for longer. By utilising LEDs as a light-source, live-cells can be imaged gently and with low phototoxicity, making the Celldiscoverer 7 ideal for long-term live experiments. Additionally, an infrared LED is used for transmitted light brightfield and phase contrast imaging through all available objectives and through a variety of sample holders.
The Zeiss Celldiscoverer 7 is a boxed imaging system suited for performing automated experiments. This inverted widefield system features automatic focusing, focus stabilisation, time-course and position setup to allow researchers to insert a sample and set up long-term experiments in minutes
The Celldiscoverer 7 has a fully temperature-controlled sample chamber with a CO2 pump, this chamber is highly stable and allows for live cells to stay healthier for longer. By utilising LEDs as a light-source for widefield imaging, live-cells can be imaged gently and with low phototoxicity, making the Celldiscoverer 7 ideal for long-term live experiments. Additionally, an infrared LED is used for transmitted light brightfield and phase contrast imaging through all available objectives and through a variety of sample holders.
Thermo Scientific CryoStar NX70 is a high-performance cryostat designed to prepare tissue
sections of frozen samples in medical and research laboratories. Specimen temperature control range is from -50oC to +10oC and blade temperature control range is from -35oC to -5oC. Section thickness can be ranged from 0.5 µm to 500 µm. Fresh or fixed frozen specimens, embedded in OCT, are suitable for cryostat sectioning. Frozen sections could be used for varying staining, such as histology stains, Immunohistochemistry (IFC) and Immunofluorescence (IF) stains.
The Phasefocus Livecyte is an inverted system for live cell imaging. This microscope has Quantitative Phase Imaging (QPI, also known as Ptychography) a label-free approach that utilises relative phase shift information to produces images of cells and tissues with high contrast and high fidelity with low phototoxicity and photobleaching. The system is also equipped with widefield fluorescence capabilities for researchers that wishes to identify cells and cellular components with high specificity.
Leica RM2135 rotary microtome is used to cut very thin cross-sections of specimens (usually 4 µm) for routine and research histology studies in the fields of biology and medicine. This instrument is designed for cutting soft and harder paraffin embedded specimens, as long as they are suitable for being cut manually. The sectioning thickness setting range can be from 1 µm to 60 µm. High quality paraffin sections could be used for varying staining, such as Haematoxylin and Eosin stain (H&E), immunohistochemistry (IHC), Immunofluorescent staining (IF) and In situ hybridization (ISH).
The BOND RX provides an easy way to automate the preparation of samples. In combination with PerkinElmer Opal™ Kit it is also useful for multiplexing allowing up to 9 fluorescent labels within a single sample.
Chemicon Advanced Tissue Arrayer ATA100 allows you to create customized arrays of multiple tissue specimens on a single histologic slide. This instrument allows to selectively remove core tissue samples from paraffin blocks and arrange those tissue samples into an array within a recipient paraffin block. The newly formed tissue array paraffin block is then ready for further histology technique processing.
The Leica DM IL microscope is used to illuminate whole cells; observing and analysing a cell as a single entity. The DM IL is an inverted epifluorescence microscope, with transmitted light LED back illumination and an EL6000 Fluoro system.
The Vectra® Polaris™ Automated Quantitative Pathology Imaging System is a new class of tissue imager which provides unparalleled speed, performance, and versatility for visualizing, analyzing, quantifying, and phenotyping immune cells in situ in Formalin Fixed Paraffin Embedded (FFPE) tissue sections and TMAs to advance disease research.
ConfocalRapid FLIMSuper-resolutionFunded by Cancer Institute NSW
Fluorescence Lifetime Imaging (FLIM) adds another dimension to conventional intensity based imaging methods. FLIM is not affected by fluctuations in the fluorescence intensity and permits to discrimination between fluorophores with similar emission spectra (and from auto-fluorescence. It can be used to probe local environmental conditions (e.g., pH value), to determine ion concentrations, to study intracellular signal transduction or to distinguish between different tissue components.
The Microtime200, manufactured by the German-based PicoQuant GmbH, is generally used in conjunction with the TCS SP5 microscope. By using the lasers from the TCS SP5, the Microtime200 is capable of measuring how fast proteins move within the cell (diffusion) and protein to protein interactions.
This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet modalities in one instrument. TIRF modality allows user to image molecules at the surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample.
EasyGel is a system designed for speeding the process of producing tissue-gel-hybridization samples. The SmartClear II system is a commercial instrument that allow fast, easy and reliable tissue clearing of tissue-gel-hybridization samples. The system uses stochastic electrotransport technology to remove lipids from tissue-gel-hybridization samples, thereby producing optically transparent samples. Samples that have been optically cleared with the SmartClear II system can be imaged by a number of light microscopy techniques such as confocal and lightsheet.
Super-resolutionTIRFWidefieldFunded by Ramaciotti Foundation
This is a total internal fluorescence (TIRF) microscope built on an inverted Nikon Ti2-E microscopeThe TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. It is equipped with piezo Z stage and Nikon Perfect Focus System 4, making it ideal for long term imaging. It is equipped with stage top incubator and objective heater as well as CO2, allowing live cell imaging. With quad filter, 4 solid state laser lines,
The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability.
The Leica TCS SP8 DLS (Digital LightSheet) is an inverted laser scanning microscope capable to be used for confocal imaging and light sheet imaging. This microscope is able to image multi-colour, live, fixed or aqueous-based cleared specimens utilising a combinations of 405, 458, 488, 514, 561 and 635 nm excitation lasers through the employment of the AOBS with spectral high quantum efficiency detectors. The advantage of the two combined features (confocal/Lightsheet) on a single equipment allow to perform, with the confocal module, local photo activation or photo bleaching where the resulting dynamics can be capture via lightsheet imaging on thick sample (up to 2 mm thick and 1 cm long).
The NanoWizard® 4 XP atomic force microscope is an advanced Atomic Force Microscope (AFM). AFM is a very high-resolution type of Scanning probe microscopy (SPM) which acquires data by “feeling” or “touching” the surface of a sample with a mechanical probe
The Bruker MultiMode® 8 (MM8) is an advanced atomic force microscope (AFM) and Scanning Tunnelling Microscope (STM). Its PeakForce Tapping technology enables exceedingly low tip-sample force interactions compared to tapping mode, making it particularly useful for delicate samples by allowing the user to control the maximal force imparted to the sample. The MM8 also features the Bruker ScanAsyst® software, automating the optimisation of scanning parameters for users to obtain quality images.