Leica TCS SP5 equipped with a Spectra-Physics Mai Tai Ti:Sapphire laser is an inverted confocal laser scanning microscope. It is designed for high-resolution fluorescence and transmitted light imaging with single or multi-photon excitation, in 2D, 3D or 4D with live or fixed cells. It can also be used for Fluorescence Recovery After Photo bleaching (FRAP), pulsed Stimulated Emission Depletion (pulsed STED) and Fluorescence Correlation Spectroscopy (FCS). In combination with the Picoquant Microtime200, it can be used for Fluorescence Correlation Spectroscopy (FCS) coupled to Fluorescence Lifetime Imaging (FLIM).
The 3i VIVO microscope is ideally suited for intravital imaging. Its large stable stage can easily accommodate sizable tissue samples along with clamps and manipulators to aid stabilization. Equipped with a spinning disk unit for high speed optical sectioning, paired with an image intensifier, this system can achieve very high frame rates making the most of every photon. Additionally, a pulsed 532nm ablation laser is available for targeted tissue damage.
The BD Influx™ cell sorter is equipped with five excitation lasers (ultraviolet 355nm, violet 405nm, blue 488nm, yellow/green 561nm and red 642nm) and has a flexible detection configuration with a number of filter set options, detecting up to 24 parameters (21 fluorescence channels, two forward scatter channels and one side scatter).