Nikon N-Storm

Nikon N-Storm

This is a total internal fluorescence (TIRF) microscope built on an inverted Nikon Ti2-E microscope The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. It is equipped with piezo Z stage and Nikon Perfect Focus System 4, making it ideal for long term imaging. It is equipped with stage top incubator and objective heater as well as CO2, allowing live cell imaging. With quad filter, 4 solid state laser lines,

Hamamatsu ORCA Flash 4.0 sCMOS Camera and Nikon SR Apo TIRF 100x objective lens (NA 1.49) makes it an ideal instrument for 3-color N-STORM. Cylindrical prism in emission path allows for 3D detection and localization of molecules within cells.

Microscope body: Inverted
Motroised Stage: Yes
Temp Control: Full Enclosure
CO2: Yes
Software: NIS-Elemements + JOBS + General Analysis

 

 

Common Applications

Live Cell Imaging:

Many biological processes are dynamic events and as such observing living cells is crucial to unravelling and understanding the mechanisms behind them. This requires special considerations when imaging. Firstly, the environmental conditions must be controllable and stable to ensure the cells remain healthy. Secondly care needs to be taken that the act of imaging has as little impact as possible on the event being observed. This microscope has fully enclosed heated chamber and CO2 supply for short or long term live cell imaging.

TIRF

Total Internal Reflection Fluorescence microscopy exploits the unique properties of an induced evanescent wave or field in a limited specimen region immediately adjacent to the interface between two media having different refractive indices. In practice, the most commonly utilized interface in the application of TIRF is the contact area between a cell and a glass coverslip or tissue culture container.

Single molecule imaging

Single molecule imaging (N-STORM or other super-resolution techniques) at the surface of a cell using TIRF microscopy. Cooled EMCCD camera is perfect for capturing single molecules at detectable signal to noise.

Objective Lenses

Magnification

Numerical Aperture

Corrections

Immersion

Working Distance

MISC.

20x

0.75

Plan Apochromat

AIR

1 mm

DIC/Pol

40x

1.25

Apochromat

Water

0.2 mm

DIC/Pol

60x

1.49

Apochromat

OIL

0.12 mm

DIC/Pol

100x

1.494

Apochromat

OIL

0.12 mm

DIC/Pol

Light Sources

Source

Wavelengths

Output Power

Laser OBIS 405

405 nm

100 mW

Laser OBIS 488

488 nm

100 mW

Laser OBIS 561

561 nm

100 mW

Laser OBIS 640

647 nm

100 mW

 

Transmission light source available for bright field imaging. 

Filters

N-STORM Filter Cube

Quad filter usable with 4 laser lines (405, 488, 561 and 647 nm) for C-STORM or other applications and a long pass dichroic at 640 nm for N-STORM applications.

Detector

Hamamatsu OCRA FLASH 4.0 V2, large format, high-speed sCMOS camera

  • 82% Q.E.
  • 2048 * 2048 pixels
  •  6.5um * 6.5um pixel size