The 3i VIVO microscope is ideally suited for intravital imaging. Its large stable stage can easily accommodate sizable tissue samples along with clamps and manipulators to aid stabilization. Equipped with a spinning disk unit for high speed optical sectioning, paired with an image intensifier, this system can achieve very high frame rates making the most of every photon. Additionally, a pulsed 532nm ablation laser is available for targeted tissue damage.
The Aperio ScanScope FL scanner is a fluorescence microscope that is used to digitalise traditional fluorescently labelled specimens, mounted on a glass slide, through a high-quality scanning process and produces, able to scan large areas. With 20X magnification you can select from up to 4 fluorescent colors (Blue, Green, red, far-red). All slides must be labelled with DAPI or Hoechst nuclear stain for calibration and focusing purposes.
The Bruker BioScope Catalyst™ Atomic Force Microscope (AFM) system can provide high resolution (nm) topographical data of biological systems such as living cells. The instrument works in tandem with an inverted optical microscope, which is also capable of fluorescence microscopy and has a heated stage for live cell imaging.
Chemicon Advanced Tissue Arrayer ATA100 allows you to create customized arrays of multiple tissue specimens on a single histologic slide. This instrument allows to selectively remove core tissue samples from paraffin blocks and arrange those tissue samples into an array within a recipient paraffin block. The newly formed tissue array paraffin block is then ready for further histology technique processing.
EasyGel is a system designed for speeding the process of producing tissue-gel-hybridization samples. The SmartClear II system is a commercial instrument that allow fast, easy and reliable tissue clearing of tissue-gel-hybridization samples. The system uses stochastic electrotransport technology to remove lipids from tissue-gel-hybridization samples, thereby producing optically transparent samples. Samples that have been optically cleared with the SmartClear II system can be imaged by a number of light microscopy techniques such as confocal and lightsheet.
The FV1200 is a filter based upright confocal system. A workhorse of our confocal systems it is well equipped for standard fixed samples where high resolution and sensitivity are required for localization based studies. Additionally as one of the few upright confocal systems within the BMIF it provides a means for visualizing water submerged opaque samples.
The IncuCyte FLR is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible, and also comes with a selection of versatile inserts allowing you to imagine your cells in a range of vessels.
This intravital microscope boasts an impressive collection of hardware. Equipped with 2 multiphoton lasers utilising a pre-chirp compensator to keep laser pulse width < 150 fs, a collection of detectors including high sensitive NDD detectors close to the objective lens, descanned PMTs, imaging cameras for fast 64 beam scanning and also LaVision BioTec's FLIM x16 TCSPC detector for rappid fluorescence lifetime measurements.
The Leica DM IL microscope is used to illuminate whole cells; observing and analysing a cell as a single entity. The DM IL is an inverted epifluorescence microscope, with transmitted light LED back illumination and an EL6000 Fluoro system.
Leica RM2135 rotary microtome is used to cut very thin cross-sections of specimens (usually 4 µm) for routine and research histology studies in the fields of biology and medicine. This instrument is designed for cutting soft and harder paraffin embedded specimens, as long as they are suitable for being cut manually. The sectioning thickness setting range can be from 1 µm to 60 µm. High quality paraffin sections could be used for varying staining, such as Haematoxylin and Eosin stain (H&E), immunohistochemistry (IHC), Immunofluorescent staining (IF) and In situ hybridization (ISH).
The Leica TCS SP8 DLS (Digital LightSheet) is an inverted laser scanning microscope capable to be used for confocal imaging and light sheet imaging. This microscope is able to image multi-colour, live, fixed or aqueous-based cleared specimens utilising a combinations of 405, 458, 488, 514, 561 and 635 nm excitation lasers through the employment of the AOBS with spectral high quantum efficiency detectors. The advantage of the two combined features (confocal/Lightsheet) on a single equipment allow to perform, with the confocal module, local photo activation or photo bleaching where the resulting dynamics can be capture via lightsheet imaging on thick sample (up to 2 mm thick and 1 cm long).
Leica TCS SP5 equipped with a Spectra-Physics Mai Tai Ti:Sapphire laser is an inverted confocal laser scanning microscope. It is designed for high-resolution fluorescence and transmitted light imaging with single or multi-photon excitation, in 2D, 3D or 4D with live or fixed cells. It can also be used for Fluorescence Recovery After Photo bleaching (FRAP), pulsed Stimulated Emission Depletion (pulsed STED) and Fluorescence Correlation Spectroscopy (FCS). In combination with the Picoquant Microtime200, it can be used for Fluorescence Correlation Spectroscopy (FCS) coupled to Fluorescence Lifetime Imaging (FLIM).
This Leica TCS SP5 confocal system is equipped for a broad range of experimental requirements. Along with a range of fixed wavelength laser lines it now has a White-Light Laser (WLL) tunable within the range x-x for precise control over fluorophore excitation. Fitted with Hybrid Dectors (HyD) for enhanced sensitivity, and interchangeable Galvano and resonant scanners, this system is suitable for applications requiring both speed and/or improved signal-to-noise. Its Gated-STED module also enables colleciton of super-resolution images allowing investigators to distinguish objects and structures beyond the conventional resolution limit of light.
The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability.
Nikon Biostation is an inverted life cell imaging microscope enclosed in an incubator. The stage and objective are fully enclosed in temperature modulated box resulting in no focus drift during imaging. The cells are kept at 37C and supplemented with 5% CO2 throughout the entire experiment. The imaging chamber can hold 35mm glass or plastic bottom dishes of 0.17um thickness. The Biostation is an ideal microscope for imaging cell migrations, wound healing, cell division and cell growth and adherence.
The Nikon Eclipse TiE is ideally suited for live cell imaging studies. With one of the best focus stabilisation devices on the market the Perfect Focus System (PFS) ensures that the focus you set at the start of the experiment will be maintained throughout its entirety. They system is fitted with several phase contrast objects making it suitable for imaging cells through a range of materials including plastic, as well as 3 high NA DIC objects for achieving astounding detail if imaging through glass.
This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet (HiLo: https://www.nature.com/articles/nmeth1171) modalities in one instrument. The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample. The system is also supplemented with NIDAQ board and control with external trigger, which combined with the versatile illumination sequence option makes this ideal microscope for complex experiments involving the correct timing of imaging following an external stimulus of cells or animal. Definite focus is perfectly controlled using Nikon PFS system making it ideal for long term live cell or animal imaging.
The Microtime200, manufactured by the German-based PicoQuant GmbH, is generally used in conjunction with the TCS SP5 microscope. By using the lasers from the TCS SP5, the Microtime200 is capable of measuring how fast proteins move within the cell (diffusion) and protein to protein interactions.
Shandon Excelsior ES Tissue Processor is used for automated fixation, dehydration, clearing and wax infiltration of specimens prior to paraffin embedding and sectioning. Subsequent processing like staining and immunostaining can then be performed on processed specimen for pathology diagnosis or research. You can process up to 222 samples at once, across 3 level of organised baskets. The samples are hold into specimen cassettes and each level of organised basket can contain up to 74 cassettes.
Thermo Scientific CryoStar NX70 is a high-performance cryostat designed to prepare tissue
sections of frozen samples in medical and research laboratories. Specimen temperature control range is from -50oC to +10oC and blade temperature control range is from -35oC to -5oC. Section thickness can be ranged from 0.5 µm to 500 µm. Fresh or fixed frozen specimens, embedded in OCT, are suitable for cryostat sectioning. Frozen sections could be used for varying staining, such as histology stains, Immunohistochemistry (IFC) and Immunofluorescence (IF) stains.
This microscope has a unique design, allowing for a great deal of customization of the sample space and experimental design. The system is designed for up to four-color total internal fluorescence (TIRF), photoactivation (PA), fluorescence resonance energy transfer (FRET), widefield correlation spectroscopy (WCS), spatio-temporal image correlation spectroscopy (STICS), and fluorescence recovery after photobleaching (FRAP) experiments, all at high frame rates. Single-molecule measurements can be enhanced with a pair of single-photon avalanche photodiodes (APDs) and a pulse correlator for fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) experiments. An environmental chamber allows for these techniques to be extended to live cell samples.
The zeiss Spinning Disk/TIRF system is a custom built instrument. Built upon an Axio Observer X.1 body it is equipped with a Yokogawa Spinning disk CSU-X1 module providing confocal imaging at rapid frame rates captured on an EM CCD camera (Quant EM) ideally suited for low light imaging. Attached to a different microscope port is a highly sensitive high frame rate sCMOS camera (Orca Flash 4) which can be used for standard epifluorescence, rapid ratiometric imaging utilising a fast excitation switching light source (Lambda DG4) or acquire fluorescence images using TIRF illumination.