Bruker Catalyst AFM

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The Bruker Bioscope Catalyst® is an advanced atomic force microscope (AFM). AFM is a very high-resolution type of Scanning probe microscopy (SPM) which acquires data by “feeling” or “touching” the surface of a sample with a mechanical probe.

The Catalyst features the Bruker PeakForce Tapping technology, PeakForce QNM (Quantitative Nanomechanical Mapping) for the rapid acquisition of a range of sample mechanical properties including elasticity, adhesion and deformation simultaneously with topography and epifluorescence wide-field microscopy.

The catalyst can be operated in air and in liquid and is particularly recommended for the study of cells, tissues, biomaterials, single molecules and membranes

Motorised Stage: XY scan range 150um
Piezoelectric scanner: z scan range 20um
Temp Control: Heated stage (up to 40C in liquid)
Deflection detection: IR superluminescent diode λ = 850nm
Software: Nanoscope 8.15

Common Applications

Topographic imaging In Complement with the classic tapping mode used for AFM topographic imaging, the Catalyst can be run using the Peakforce tapping which operates in non-resonant and combines the benefits of tapping and contact mode imaging. Peakforce tapping also limits the damage of very soft samples by accurate control of the force set point (from pN to nN). ScanAsyst auto control mode automatically optimizes and adjusts imaging parameters in real-time, for dried samples in air, or for samples immersed in a fluid, making the experience more user-friendly.
Mechanical properties on soft samples The AFM supports measurements in PeakForce QNM (Quantitative Nanomechanical Mapping), Quantitative Force-Volume Mapping, and Single Force Curve Imaging. QNM allows for the simultaneous acquisition of multiple sample properties including topology, modulus, adhesion and deformation. The PeakForce Capture™ feature allows for the capture and analysis of individual force curves at each pixel of the image. These features have been used to image cells, tissues and biomaterials such as hydrogels.
Miro imaging The Bruker BioScope Catalyst AFM is positioned on a Leica inverted epifluorescence microscope, allowing for a real-time import and registration of optical images. Optical images can be used to guide AFM imaging of force measurements, offline features allow AFM and optical image overlay.
Single molecules and membranes The AFM high resolution allows for the imaging and force spectroscopy of Proteins, other biomolecules, and biomolecular assemblies as well as lipid monolayers and bilayers.

Objective Lenses

MAG N.A HCX FL Plan CORRECTIONS
10x 0.25 HCX FL Plan Dry
20x 0.4 HCX FL Plan Dry
63x 1.2 XCX PL APO Water
100x 1.4 XCX PL APO Oil

Light Sources and Filter Cubes

Light source: Leica Mercury lamp

 

Filter Cubes: A, I3, YFP, Cy3 & Cy5