Lavision TriM Scope II Multiphoton Intravital

 
 
This intravital microscope boasts an impressive collection of hardware. Equipped with 2 multiphoton lasers utilising a pre-chirp compensator to keep laser pulse width < 150 fs, a collection of detectors including high sensitive NDD detectors close to the objective lens, descanned PMTs, imaging cameras for fast 64 beam scanning and also LaVision BioTec's FLIM x16 TCSPC detector for rappid fluorescence lifetime measurements. 
Microscope body:
Upright
Motorised Stage:
No; Hydrolic Intravital
Temp. Conotrol:
No
CO2:
No
Software:
Imspector 5

Common Applications

Intravital Imaging Intravital imaging is the observation of biological processes in living animals/tissue. It introduces new challenges such as achieving higher penetration depth, and dealing with sample mobility. Appreciating that how cells respond in tissue culture has at times been shown to be largely different from their response in their true environment makes intravital imaging a cruical step in understanding biological systems.
Multiphoton Excitation  Multiphoton excitation is the use of lower energy wavelengths to excite a fluorophore by requiring multiple photons to coincide to provid the required excitation energy. This yields two main advantages. Firstly the higher density of photons at the focal point effectively restricts excitation to this region, preventing bleaching of regions out of the focal plane. Secondly, the longer wavelengths scatter less through tissue improving excitation penetration depths. 
FLIM Fluorescence Lifetime Imageing Microscopy is not only observing where fluorescence light is emitted within the sample, but also measuring the characteristic time delay between excitation and the detection of the emitted light. Changes in the time it takes for a fluorophore to emit light after excitation (Fluorescence Lifetime) can be indicative of molecular interactions, and changing environmental conditions.

Objective Lenses

MAG N.A CORRECTIONS IMMERSION W.D MISC.
4x 0.28 XL Fluor 4x AIR 29.5 mm  
5x 0.15   AIR 20.0 mm  
10x 0.3 UMPlan FL WATER 3.3 mm  
20x 0.5   WATER 3.5 mm  
20x 1.0 W Plan Apochromat WATER 1.7 mm DIC (UV) Vis-IR
32x 0.85   WATER 1.1 mm  
40x 0.8 LumPlan FL N WATER 3.3 mm  
N.A: Numerical Apperture
W.D: Working Distance

Light Sources

Tunning range: 690 - 1040 nm
Repetition rate: 80 MHz +- 1 MHz
 
Tunning range: 680 - 1300 nm
Repetition rate: 80 MHz +- 1 MHz
pulse width < 120 fs

 

Filters

Detectors