Nikon Eclipse Tie

The Nikon Eclipse TiE is ideally suited for live cell imaging studies. With one of the best focus stabilisation devices on the market the Perfect Focus System (PFS) ensures that the focus you set at the start of the experiment will be maintained throughout its entirety. They system is fitted with several phase contrast objects making it suitable for imaging cells through a range of materials including plastic, as well as 3 high NA DIC objects for achieving astounding detail if imaging through glass.
Microscope body:
Inverted
Motroised Stage:
Yes
Temp Control:
Stage Insert
CO2:
Stage Insert
Software:
NIS Elements V4

Common Applications

Live Cell Imaging: Many biological processes are dynamic events and as such observing living cells is cruicial to unravelling and understanding the mechanisms behind them. This requires special considerations when imaging. Firstly the environmental conditions must be controllable and stable to ensure the cells remain healthy. Secondly care needs to be taken that the act of imaging has as little impact as possible on the event being observed.
Cell Migration: This broad term is use to refer to any processes that involve the translation of cells from one location to another. This may include development, wound healing, invasion of cancer cells, immune response, etc.
Cell Proliferation: Measuring the rate of cell proliferation can be a simple readout for assessing cell viability. It can be use for the screening of drugs expected to induce cell death or senescence.

Objective Lenses

MAG N.A CORRECTIONS IMMERSION W.D MISC.
10x 0.3 Plan Fluor AIR 15.2 mm Phase
20x 0.45 S Plan Fluor AIR 8.2 mm Phase
20x 0.75 Plan Apochromat AIR 1 mm DIC
40x 0.6 Plan Fluor ELWD AIR 3.7 mm Phase
40x 0.95 Plan Apochromat AIR 0.14 mm DIC
60x 1.2 Plan Apochromat WATER 0.31 mm DIC

Light Sources

SOURCE WAVELENGTHS
Mercury Lamp 400 - 600 nm
Halogen Lamp Visible transmitted light
 

Filters

In addition to the filter turret containing a DIC analyser there are also 5 filter cubes for fluorescence imaging.
 
NAME EXCITATION DICHROIC EMISSION
DAPI BP 381 - 392 nm 409 nm BP 417 - 477 nm
CFP BP 426 - 450 nm 458 nm BP 463 - 499 nm
GFP BP 468 - 496 nm 495 nm BP 506 - 534 nm
YFP BP 488 - 512 nm 510 nm BP 528 - 556 nm
Texas Red BP 542 - 582 nm 593 nm BP 604 - 644 nm

 

Detectors

The images are recorded by 12 bit Charged Coupled Device (CCD) camera with maximum pixel area of 2048 x 2048