|FRAP:||Fluorescence Recovery After Photobleaching is used to gain insight into the dynamics or both diffusion and binding rates within a sample. Photobleaching is an irreversible process by which a fluorophore loses the ability to absorb and emit light. By deliberately inducing photobleaching within a restricted region of the sample observing the recovery within this region over time is affected by the rate at which unbleached molecules entre this region and bleached molecules are moved.|
FRET (Fluorescence Resonance Energy Transfer) is a technique used largely to investigate molecular interactions between two or more molecules. It works by using two carefully selected fluorophores that when in close enough proximity (10–100 Å), and suitable orientation, energy is transferred nonradiatively from the excited donor fluorophore to another acceptor fluorophore.
|Photo-activation within live cells:||
Photoactivation is a technique of photomanipulation used to investigate intracellular protein movement. A laser is used to cause a structural change to a population of molecules like fluorophores. These photoactivated molecules can then be tracked to assess their movements.