Zeiss Axio Observer X.1 SD & TIRF

Zeiss Axio Observer X.1 SD & TIRF


The zeiss Spinning Disk/TIRF system is a custom built instrument.  Built upon an Axio Observer X.1 body it is equipped with a Yokogawa Spinning disk CSU-X1 module providing confocal imaging at rapid frame rates captured on an EM CCD camera (Quant EM) ideally suited for low light imaging. Attached to a different microscope port is a highly sensitive high frame rate sCMOS camera (Orca Flash 4) which can be used for standard epifluorescence, rapid ratiometric imaging utilising a fast excitation switching light source (Lambda DG4) or acquire fluorescence images using TIRF illumination. 
Microscope body:
Motorised Stage:
Temp. Control:
Full Enclosure
Stage insert
Zen 2012










Common Applications









Epi-Fluorescence Conventional widefield fluorescent imaging.......
TIRF  Total Internal Reflection Florescence microscopy is a technique by which optical sectioning is achieved by restrictuing fluorphore excitation to within a few nundred nanometer of the glass/water interface. This technique is very useful for observing the basel membrane of adherent and migratory cells, as well as observing single molecules on glass surfaces.
Spinning Disk Spinning Disk microscopy allows fast confocal-like optical sectioning. By utilising multiple pinholes in a rotating disk several points can be scanned in parallel enabling rapid acquisition. Especially useful when trying to observe fast biological processes, such as vesicle transport

Ratiometric Calcium Imaging

Calcium imaging is a technique by which a flourophore whose properties are sensitive to calcium concentrations is used to report on dynamic changes in intracellular calcium levels. Simple intensity based techniques can suffer from several complications such as florescence bleaching and changes in cell morphology. Ratiometric approaches solve this issue but require very rapid instrumentation to yield accurate results. This instrument is equipped with a rapid light source switcher (lambda DG4) and a scientific scCMOS camera whose read out time is under 1ms.










Objective Lenses









20x 0.4 LD Plan Neofluar AIR 7.9mm  
40x 0.75 EC Plan Neofluar AIR 0.71mm DIC
40x 1.3 EC Plan Neofluar OIL 0.2mm DIC
63x 1.3 LCI Plan Neofluar Water/Glycerol 0.17mm DIC
63x 1.4 Plan Apochromat OIL 0.19mm DIC
100x 1.46 alpha Plan Apochrmat OIL 0.11mm TIRF, DIC, (UV)
* Click on objective magnification for manufactures specification sheet. 










Light Sources










Diode: 405nm

Argon ion: 458nm 488nm 514nm


Xenon  Lambda DG-4: 300 Watt Xenon arc bulb




















Epifluorescence & TIRF
Name Excitation Dicrhoic Emission

BP 325 - 355

BP 379 - 395

BS 410 BP 465 - 555
43 HE DsRED BP 538 - 562 BS 570 BP 570 - 640

BP 464 - 496

BP 552 - 577

BS 505


BP 510 - 540

BP 588 - 645


Spinning Disk
Dichroics Emission Filters



BP 450/50

BP 485/30

BP 525/50

BP 629/62



















Detector Pixel Format SeNsor Description
Orca Flash 4 V2 2048 x 2048 sCMOS ???????
QuantEM 512SC 512 x 512 emCCD ?????????


Test Document


Contact People

Dr Renee M Whan

Position: Head of Biomedical Imaging Facility

+61 -2-93859342
+61 409 811 073
Dr Alex Macmillan

Position: Lecturer

+61 2 9385 1721
+61 499 090 805
Michael Carnell
Dr Michael Carnell

Position: Research Associate

+61 2 9385 2973
+61 499 050 287