Zeiss LSM 900

Zeiss LSM 900
This LSM 900 is an inverted high-end confocal microscope. Equipped with GaAsP detectors and an Airyscan 2 detector it delivers speed and sensitivity without compromising signal to noise. A new multiplex mode for Airyscan 2 gives you the ability to choose between an increase in resolution by 1.7x, improve your signal to noise, or increase your acquisition speed. The acquisition software can acquire and stitch tiled images of samples too large to fit within the field of view. It can easily be programmed to capture images of multiple regions on a slide or multi-well plate
Microscope body: Inveted
Motorised Stage: Yes
Temp Control: Full Enclosure
CO2: Stage Insert
Software: Zen Blue 3.1

Common Applications

Widefield transmitted and epifluorescence This microscope is equipped with a monochromatic camera to allow quick and gentle overview caption of large area of your sample before imaging objects of interest at higher resolution. Short term live cell imaging in both transmitted and epifluorescence widefield mode is also possible with this system.
Confocal Confocal imaging is a fluorescence microscopy technique that optically sections the specimen, preventing ‘out of focus’ light from reaching the detector. This yields clear and high contrast images, and together with the ability to acquire images sequentially at multiple positions, the entire sample can be reconstructed in 3D.
Colocalisation Light microscopy lends itself very well to labelling multiple structures within a sample due to the ability to separate these spatially overlapping signals by the wavelength of light they emit. Colocalisation is the study of how the distribution of one probe relates to another within the same sample.
Enhanced resolution (Airyscan): The Airyscan detector consists of a hexagonal array of 32 GaAsP/PMT detector elements that have the light collection efficiency of a 1.25 AU pinhole (up to 50% more light collected than with a 1 AU pinhole). Each detector element functions similarly to a single, small (0.2 AU) pinhole, which enables the generation of higher (1.7 x) resolution images with improved signal-to-noise ratio than a standard confocal. The Fast mode can be used to increase acquisition speed to up to 13 fps by elongating the excitation beam to acquire 4 lines of image at once, without sacrificing resolution or signal-to-noise.
Short term live cell culture: Live cells can be imaged in an automated manner using the Experiment Designer software tool, combined with three parallel spectral channels. The motility of subcellular structures can be studied using Airyscan GaAsP detector to image with time lapse imaging in 2D or 3D at 1.6 frames per second

Objective Lenses

MAG

N.A.

CORRECTIONS

IMMERSION

 

W.D.

5x

0.16

Plan-Apochromat

Air

 

 

10x

0.45

Plan-Apochromat

Air

 

2mm

20x

0.8

Plan-Apochromat

Air

 

0.55 mm

40x

1.3

Plan-Apochromat

Oil

 

0.21 mm

63x

1.4

Plan-Apochromat

Oil

 

0.19 mm

Light Sources

For Widefield

Source: Colibri 5/7 

Wavelengths 

Nominal Power 

LED 385 

385 nm 

 

LED 430 

430 nm 

 

LED 475 

475 nm 

 

LED 511 

511 nm 

 

LED 567 

555 nm   

& 590 nm 

Excitation filter 555 nm and 590 nm 

LED 630 

630 nm 

 

For Confocal

Source 

Wavelengths 

Nominal Power 

405 Laser 

405 nm 

5 mW 

488 Laser 

488 nm 

10 mW 

561 Laser 

561 nm 

10 mW 

640 Laser 

640 nm 

5 mW 

Filters

NAME 

EXCITATION 

DICHROIC 

EMISSION 

Filter Set 50 (Cy5) 

BP 625 - 655 

BS 660 

BP 665 - 715 

Filter Set 91 (HE CFP/YFP/mCherry) 

BP 406 – 440 

BP 494 – 538 

BP 583 - 600 

BS 450 

BS 538 

BS 610  

BP 458 – 474 

BP 546- 564 

BP 618- 756 

Filter Set 109 (HE DAPI/GFP/DsRed) 

BP 375 – 395 

BP 455 – 438 

BP 545 565 

BS 405 

BS 493 

BS 575 

BP 412 – 438 

BP 501 – 527 

BP 585 - 675 

Detectors

Widefield detector: 

Axiomcam506m_S1679 monochromatic camera, readout speed 39MHz 

Confocal detector: 

  • 2x  GaAsP-PMT  
  • 1x Airyscan detector GaAsP-PMT (32 channels GaAsP) optimised for 63× objective 
  • 1x Transmitted light detector (T-PMT) 

Scanning and Stage

Name 

Range 

Description 

Scanning stage 130x100 STEP  

130 mm x 100 mm travel range 

Motorized stage (maximum speed 50 mm/s) for 3D imaging (maximum 250g) 

Galvanometric scanner 

 

8 Frames/s at 512x512. Maximum frame resolution: 6144x6144, with Airyscan: 4096 x 4096 pixel adjustable for each axis  

Miscellaneous

< 8 sequential confocal fluorescence channels, up to three parallel confocal fluorescence channels, based on low-noise GaAsp or MA PMTs 

Microscope maximum Lateral resolution 120 nm, axial resolution 350 nm