Liquid Chromatography

Separation and Fractionation

Separation methods such as gas and liquid chromatography (GC & LC) are essential in most applications and these are mostly performed on-line with the mass spectrometer. For proteomic analysis the use of very narrow columns in the nanoflow regime (for nanospray - a scaled down version of electrospray) is widely practised at the BMSF including the use of sophisticated 2-dimensional separations (LC-LC). Techniques such as 1 dimensional and 2 dimensional gel eletrophoresis (SDS PAGE & 2DE) are important off-line methods for large biomolecules such as proteins and nucleic acids. The BMSF has a comprehensive range of separation devices and mass spectrometers to address most analytical problems suited to mass spectrometry.

Searching all LC instruments. Search all instruments.

Show
Sort by  Alphabetical  |  Frequently Used
results per page

Agilent(HP) 1090 Micro LC and Prep LC

The HP 1090 HPLC system is useful for a wide range of applications for metabolites, peptides and proteins including: sample clean up; enrichment, and fractionation. With flow rates of between 50–5000 μl/min it suits elution using narrow bore to analytical size columns that can be detected with a UV detector. Columns can be temperature controlled and there are also 3 solvent reservoirs for solvent delivery binary, ternary or gradient elution. The application of this instrument to many kinds of separation columns is the advantage of this stand alone LC system.

Read more

AKTA FPLC

The GE AKTA Purifier LC system and fraction collector is used predominantly for protein purification, as it has no metal parts. The system has a manual injector, with 3 channel UV and conductivity detectors. The Solvent delivery system is capable of delivering both isocratic and gradient elution, and can be used for both analytical and semi-preparative work and fractionation depending on choice of column used. Major work projects have included the purification and fractionation of cytochrome C and related products.

Read more

Shimadzu HPLC

The Shimadzu Prominence HPLC system is used for both analytical and semi-preparative separations. The instrument is equipped with fluorescence, photo diode array (PDA), and evaporative light scattering (ELSD) detectors, and is combined with a fraction collector. The instrument has a multitude of set-ups and combination and is capable of delivering both isocratic and gradient solvent elution from 0.2 to 10.5 mL/minute.

Read more

Shimadzu UFLCxr

The Shimadzu XR Prominence HPLC system is used for analytical separations. The instrument has a binary pump set-up to allow both isocratic and gradient elution at high back pressures (circa 14 000 Psi), and is equipped with a photo diode array detector (PDA). A wide range of separations are performed on the instrument encompassing both normal and reverse phase conditions, with a wide variety of column chemistries utilised for identification and quantitative analysis.

Read more

results per page