Separation and Fractionation
Separation methods such as gas and liquid chromatography (GC & LC) are essential in most applications and these are mostly performed on-line with the mass spectrometer. For proteomic analysis the use of very narrow columns in the nanoflow regime (for nanospray - a scaled down version of electrospray) is widely practised at the BMSF including the use of sophisticated 2-dimensional separations (LC-LC). Techniques such as 1 dimensional and 2 dimensional gel eletrophoresis (SDS PAGE & 2DE) are important off-line methods for large biomolecules such as proteins and nucleic acids. The BMSF has a comprehensive range of separation devices and mass spectrometers to address most analytical problems suited to mass spectrometry.