Matrix Assisted Laser Desorption / Ionisation

Proteins, Polymers and other High MW or Polar Molecules including Proteomics

LC/MS is now a mature method of analysis and it usually is coupled with electrospray ionisation (ESI) or atmospheric pressure chemical ionisation (APCI). Tandem mass spectrometry (MS/MS) is usually applied with these methods to facilitate structural characterisation of many types of compounds (including synthetic polymers) as well as to tailor a quantitative method that detects very low levels of a target analyte in highly complex mixtures. In addition to several LC/MS instruments, the BMSF is equipped with matrix assisted laser desorption/ionisation (MALDI) instruments which analyse samples deposited as solids on a plate. The BMSF is well equipped for the analysis of proteins, lipids, carbohydrates and other biopolymers. The BMSF is particularly experienced in protein identification using modern proteomic methods. In addition staff at the BMSF practice a wide range of quantitative proteomic analysis using isotopic labelling strategies to measure relative protein experession levels between samples and controls.