Flow Cytometry

Flow Cytometry is a technology that simultaneously measures and analyses multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. The equipment can both analyse and sort single cells based on these characteristics and researchers utilize this service for applications in immunology, cell biology, microbiology and many more.

The BRIL Flow Cytometry facility provides comprehensive and technical cell analysis and sorting services to groups within the campus and the wider Sydney research basin. The facility currently incorporates Analysis instruments; BD LSRFortessa™ (5 laser), BD FACS Canto II™ (x2) and access to three cell-sorting instruments; BD Influx™ (5 laser), BD FACS Jazz™ (2 laser) and BD FACSAria™II (upgraded). Basic information on instruments, capabilities and configurations are listed under 'Techniques' as well as their respective links to company websites containing more detailed information. Cell sorting services are provided in both FFS (fee for service) and DIY (do it yourself) systems.

If you require clarification or have queries about specific applications, please contact Chris Brownlee or David Snowden.