Dr Nicholas Ariotti

Associate Director

Category: Academic Staff

Biography

Education

PhD - Cell and Membrane Biology - The University of Queensland (2013)

BSc (Hons) - The University of Queensland (2006)

Role and Interests

My role is to provide training and support for clients in all facets of biological electron microscopy including sample preparation techniques, correlative light and electron microscopy and cryo-electron microscopy.

My research focuses on understanding how proteins are organised within the lipid bilayer of the plasma membrane and how changes in this organisation can result in disease.

Publications

Follett J, Bugarcic A, Yang Z, Ariotti N, Norwood SJ, Collins BM, Parton RG and Teasdale RD (2015). Parkinson’s disease linked Vps35 R524W mutation impairs retromer’s endosomal association and induces α-synuclein inclusion formation. Accepted: The Journal of Biological Chemistry.

Blaževitš O, Mideksa YG, Šolman M, Ligabue A, Ariotti N, Nakhaeizadeh H, Fansa EK, Papageorgiou AC, Wittinghofer A, Ahmadian MR and Abankwa D (2016). Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering. Scientific Reports 6, 24165. doi: 10.1038/srep24165

Hall TE*, Ariotti N*, Ferguson C, Xiong Z and Parton RG (2016). New Transgenic lines for localization of GFP-tagged proteins by electron microscopy. Zebrafish 13, 232-3. *Equal contribution.

Ariotti N, Hall TE, Rae J, Ferguson C, Martel N, Webb R, McMahon K, Webb RI, Teasdale RD and Parton RG (2015). Modular detection of GFP-labeled proteins for rapid screening by electron microscopy in cells and animals. Developmental Cell 35, 513-25. doi: 10.1016/j.devcel.2015.10.016

Ariotti N, Rae J, Leneva N, Ferguson C, Loo D, Okano S, Hill MM, Walser PJ, Collins BM and Parton RG (2015). Molecular characterization of caveolin-induced membrane curvature. The Journal of Biological Chemistry 2015 August 24. doi: 10.1074/jbc.M115.644336.

Kovtun O, Tillu VA, Ariotti N, Parton RG and Collins BM (2015). Cavin family proteins and the assembly of caveolae. Journal of Cell Science 123(7), 1269-1278.

Kovtun O, Tillu VA, Jung WR, Leneva N, Ariotti N, Chaudhary N, Mandyam RA, Ferguson C, Morgan GP, Johnston WA, Harrop SJ, Alexandrov K, Parton RG and Collins BM (2014). Structural insights into the organization of the cavin membrane coat complex. Developmental Cell 31, 405-19.

Lakshminarayan R, Wunder C, Becken U, Howes MT, Benzing C, Arumugam S, Sales S, Ariotti N, Chambon V, Lamaze C, Loew D, Shevchenko A, Gaus K, Parton RG and Johannes L. (2014). Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers. Nature Cell Biology 16, 595-606.

Ariotti N, Fernandez-Rojo MA, Zhou Y, Hill MM, Rodkey TL, Inder KI, Tanner LB, Wenk MR, Hancock JF and Parton RG (2014). Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling. The Journal of Cell Biology 204, 777-92.

Zhou Y, Liang H, Rodkey T, Ariotti N, Parton RG and Hancock JF (2014). Signal integration by lipid-mediated spatial cross talk between Ras nanoclusters. Molecular and Cellular Biology 34, 862-76.

Ariotti N and Parton RG (2013). Snapshot: Caveolae, caveolins, and cavins. Cell 15, 704-704.

Gambin Y, Ariotti N, McMahon K, Bastiani M, Sierecki E, Kovtun O, Magenau A, Jung W, Okano S, Zhou Y, Mureev S, Johnston W, Gaus K, Hancock JF, Alexandrov K and Parton RG (2013). Single-molecule analysis reveals self-assembly and nanoscale segregation of two distinct subcomplexes on caveolae. eLife 3, e01434.

Herms A, Bosch M, Ariotti N, Reddy BJN, Fajardo A, Fernández-Vidal A, Alvarez-Guaita A, Fernández-Rojo MA, Rentero C, Tebar F, Enrich C, Geli M, Parton RG, Gross SP and Pol A (2013). Cell to cell lipid droplet heterogeneity: a population advantage to reduce lipotoxicity. Current Biology 23, 1489-96.

Walser PJ, Ariotti N, Howes MT, Ferguson C, Webb R, Schwudke D, Leneva N, Cho KJ, Cooper L, Rae J, Floetenmeyer M, Oorschot VM, Skoglund U, Simons K, Hancock JF and Parton RG (2012). Constitutive formation of caveolae in a bacterium. Cell 150, 752-63.

Ariotti N, Murphy S, Hamilton NA, Wu L, Green K, Schieber NL, Li P, Martin S and Parton RG (2012). Post-lipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes. Molecular Biology of the Cell 23, 1826-37.

Köhnke M, Schmitt S, Ariotti N, Piggott AM, Parton RG, Lacey E, Capon RJ, Alexandrov K and Abankwa D (2012). Design and application of in vivo FRET-biosensors to identify protein prenylation and nanoclustering inhibitors. Chemistry & Biology 19, 866-74.

Fairn GD, Schieber NL, Ariotti N, Murphy S, Kuerschner L, Webb RI, Grinstein S and Parton RG (2011). High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine. Journal of Cell Biology 194, 257-75.

Ariotti N, Liang H, Xu Y, Zhang Y, Yonekubo Y, Inder K, Du G, Parton RG, Hancock JF and Plowman SJ (2010). Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formation. Molecular and Cellular Biology 30, 3795-804.

Shalom-Feuerstein R, Plowman SJ, Rotblat B, Ariotti N, Tian T, Hancock JF and Kloog Y (2008). K-ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3. Cancer Research 68, 6608-16.

Plowman SJ, Ariotti N, Goodall A, Parton RG and Hancock JF (2008). Electrostatic interactions positively regulate K-Ras nanocluster formation and function. Molecular and Cellular Biology 28, 4377-85.

Abankwa D, Hanzal-Bayer M, Ariotti N, Plowman SJ, Gorfe AA, Parton RG, McCammon JA and Hancock JF (2008). A novel switch region regulates H-ras membrane orientation and signal output. EMBO Journal 27, 727- 35

My Research Activities

Education

PhD - Cell and Membrane Biology - The University of Queensland (2013)

BSc (Hons) - The University of Queensland (2006)

Role and Interests

My role is to provide training and support for clients in all facets of biological electron microscopy including sample preparation techniques, correlative light and electron microscopy and cryo-electron microscopy.

My research focuses on understanding how proteins are organised within the lipid bilayer of the plasma membrane and how changes in this organisation can result in disease.

Publications

Publications

Conference Papers

McGhee J;Bailey B;Parton RG;Ariotti N;Johnston A, 2016, 'Journey to the Centre of the cell (JTCC): A 3D VR experience derived from migratory breast cancer cell image data', in SA 2016 - SIGGRAPH ASIA 2016 VR Showcase, http://dx.doi.org/10.1145/2996376.2996385

Journal articles

Cui Y;Carosi JM;Yang Z;Ariotti N;Kerr MC;Parton RG;Sargeant TJ;Teasdale RD, 2019, 'Retromer has a selective function in cargo sorting via endosome transport carriers', Journal of Cell Biology, vol. 218, pp. 615 - 631, http://dx.doi.org/10.1083/jcb.201806153
View more
Golani G;Ariotti N;Parton RG;Kozlov MM, 2019, 'Membrane Curvature and Tension Control the Formation and Collapse of Caveolar Superstructures', Developmental Cell, vol. 48, pp. 523 - 538.e4, http://dx.doi.org/10.1016/j.devcel.2018.12.005
Jung WR;Sierecki E;Bastiani M;O'Carroll A;Alexandrov K;Rae J;Johnston W;Hunter DJ B;Ferguson C;Gambin Y;Ariotti N;Parton RG, 2018, 'Cell-free formation and interactome analysis of caveolae', Journal of Cell Biology, vol. 217, pp. 2141 - 2165, http://dx.doi.org/10.1083/jcb.201707004
Johnston AP R;Rae J;Ariotti N;Bailey B;Lilja A;Webb R;Ferguson C;Maher S;Davis TP;Webb RI;McGhee J;Parton RG, 2018, 'Journey to the centre of the cell: Virtual reality immersion into scientific data', Traffic, vol. 19, pp. 105 - 110, http://dx.doi.org/10.1111/tra.12538
Tavallaie R;McCarroll J;Le Grand M;Ariotti N;Schuhmann W;Bakker E;Tilley RD;Hibbert DB;Kavallaris M;Gooding JJ, 2018, 'Nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood', Nature Nanotechnology, vol. 13, pp. 1066 - 1071, http://dx.doi.org/10.1038/s41565-018-0232-x
Kovtun O;Leneva N;Bykov YS;Ariotti N;Teasdale RD;Schaffer M;Engel BD;Owen DJ;Briggs JA G;Collins BM, 2018, 'Structure of the membrane-assembled retromer coat determined by cryo-electron tomography', Nature, vol. 561, pp. 561 - 564, http://dx.doi.org/10.1038/s41586-018-0526-z
Ariotti N;Rae J;Giles N;Martel N;Sierecki E;Gambin Y;Hall TE;Parton RG, 2018, 'Ultrastructural localisation of protein interactions using conditionally stable nanobodies', PLoS Biology, vol. 16, http://dx.doi.org/10.1371/journal.pbio.2005473
Mehmood R;Ariotti N;Yang JL;Koshy P;Sorrell CC, 2018, 'PH-Responsive Morphology-Controlled Redox Behavior and Cellular Uptake of Nanoceria in Fibrosarcoma', ACS Biomaterials Science and Engineering, vol. 4, pp. 1064 - 1072, http://dx.doi.org/10.1021/acsbiomaterials.7b00806
Zhang Y;Ingham B;Cheong S;Ariotti N;Tilley RD;Naffa R;Holmes G;Clarke DJ;Prabakar S, 2018, 'Real-Time Synchrotron Small-Angle X-ray Scattering Studies of Collagen Structure during Leather Processing', Industrial and Engineering Chemistry Research, vol. 57, pp. 63 - 69, http://dx.doi.org/10.1021/acs.iecr.7b03860
Ariotti N;Hall TE;Parton RG, 2017, 'Correlative light and electron microscopic detection of GFP-labeled proteins using modular APEX', Methods in Cell Biology, vol. 140, pp. 105 - 121, http://dx.doi.org/10.1016/bs.mcb.2017.03.002
Sierecki E;Giles N;Bowden Q;Polinkovsky ME;Steinbeck J;Arrioti N;Rahman D;Bhumkar A;Nicovich PR;Ross I;Parton RG;Böcking T;Gambin Y, 2016, 'Nanomolar oligomerization and selective co-aggregation of α-synuclein pathogenic mutants revealed by single-molecule fluorescence', Scientific Reports, vol. 6, http://dx.doi.org/10.1038/srep37630
Blaževitš O;Mideksa YG;Šolman M;Ligabue A;Ariotti N;Nakhaeizadeh H;Fansa EK;Papageorgiou AC;Wittinghofer A;Ahmadian MR;Abankwa D, 2016, 'Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering', Scientific Reports, vol. 6, http://dx.doi.org/10.1038/srep24165
Hall TE;Ariotti N;Ferguson C;Xiong Z;Parton RG, 2016, 'New Transgenic Lines for Localization of GFP-Tagged Proteins by Electron Microscopy', Zebrafish, vol. 13, pp. 232 - 233, http://dx.doi.org/10.1089/zeb.2016.29002.hal
Follett J;Bugarcic A;Yang Z;Ariotti N;Norwood SJ;Collins BM;Parton RG;Teasdale RD, 2016, 'Parkinson disease-linked Vps35 R524W mutation impairs the endosomal association of retromer and induces α-synuclein aggregation', Journal of Biological Chemistry, vol. 291, pp. 18283 - 18298, http://dx.doi.org/10.1074/jbc.M115.703157
Kovtun O;Tillu VA;Ariotti N;Parton RG;Collins BM, 2015, 'Cavin family proteins and the assembly of caveolae', Journal of Cell Science, vol. 128, pp. 1269 - 1278, http://dx.doi.org/10.1242/jcs.167866
Ariotti N;Rae J;Leneva N;Ferguson C;Loo D;Okano S;Hill MM;Walser P;Collins BM;Parton RG, 2015, 'Molecular characterization of caveolin-induced membrane curvature', Journal of Biological Chemistry, vol. 290, pp. 24875 - 24890, http://dx.doi.org/10.1074/jbc.M115.644336
Ariotti N;Hall TE;Rae J;Ferguson C;McMahon KA;Martel N;Webb RE;Webb RI;Teasdale RD;Parton RG, 2015, 'Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms', Developmental Cell, vol. 35, pp. 513 - 525, http://dx.doi.org/10.1016/j.devcel.2015.10.016
Zhou Y;Liang H;Rodkey T;Ariotti N;Parton RG;Hancock JF, 2014, 'Signal integration by lipid-mediated spatial cross talk between ras nanoclusters', Molecular and Cellular Biology, vol. 34, pp. 862 - 876, http://dx.doi.org/10.1128/MCB.01227-13
Ariotti N;Fernández-Rojo MA;Zhou Y;Hill MM;Rodkey TL;Inder KL;Tanner LB;Wenk MR;Hancock JF;Parton RG, 2014, 'Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling', Journal of Cell Biology, vol. 204, pp. 777 - 792, http://dx.doi.org/10.1083/jcb.201307055
Kovtun O;Tillu VA;Jung WR;Leneva N;Ariotti N;Chaudhary N;Mandyam RA;Ferguson C;Morgan GP;Johnston WA;Harrop SJ;Alexandrov K;Parton RG;Collins BM, 2014, 'Structural insights into the organization of the cavin membrane coat complex', Developmental Cell, vol. 31, pp. 405 - 419, http://dx.doi.org/10.1016/j.devcel.2014.10.002
Gambin Y;Ariotti N;McMahon KA;Bastiani M;Sierecki E;Kovtun O;Polinkovsky ME;Magenau A;Jung WR;Okano S;Zhou Y;Leneva N;Mureev S;Johnston W;Gaus K;Hancock JF;Collins BM;Alexandrov K;Parton RG, 2014, 'Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae', eLife, vol. 2014, http://dx.doi.org/10.7554/eLife.01434.001
Gambin Y;Ariotti N;McMahon KA;Bastiani M;Sierecki E;Kovtun O;Polinkovsky ME;Magenau A;Jung W;Okano S;Zhou Y;Leneva N;Mureev S;Johnston W;Gaus K;Hancock JF;Collins BM;Alexandrov K;Parton RG, 2014, 'Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae', eLife, vol. 3, pp. e01434, http://dx.doi.org/10.7554/eLife.01434
Lakshminarayan R;Wunder C;Becken U;Howes MT;Benzing C;Arumugam S;Sales S;Ariotti N;Chambon V;Lamaze C;Loew D;Shevchenko A;Gaus K;Parton RG;Johannes L, 2014, 'Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers.', Nature cell biology, vol. 16, pp. 595 - 606, http://dx.doi.org/10.1038/ncb2970
Ariotti N;Parton RG, 2013, 'SnapShot: caveolae, caveolins, and cavins.', Cell, vol. 154
Herms A;Bosch M;Ariotti N;Reddy BJ N;Fajardo A;Fernández-Vidal A;Alvarez-Guaita A;Fernández-Rojo MA;Rentero C;Tebar F;Enrich C;Geli MI;Parton RG;Gross SP;Pol A, 2013, 'Cell-to-cell heterogeneity in lipid droplets suggests a mechanism to reduce lipotoxicity', Current Biology, vol. 23, pp. 1489 - 1496, http://dx.doi.org/10.1016/j.cub.2013.06.032
Ariotti N;Murphy S;Hamilton NA;Wu L;Green K;Schieber NL;Li P;Martin S;Parton RG, 2012, 'Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes', Molecular Biology of the Cell, vol. 23, pp. 1826 - 1837, http://dx.doi.org/10.1091/mbc.E11-10-0847
Köhnke M;Schmitt S;Ariotti N;Piggott AM;Parton RG;Lacey E;Capon RJ;Alexandrov K;Abankwa D, 2012, 'Design and application of in vivo FRET biosensors to identify protein prenylation and nanoclustering inhibitors', Chemistry and Biology, vol. 19, pp. 866 - 874, http://dx.doi.org/10.1016/j.chembiol.2012.05.019
Walser PJ;Ariotti N;Howes M;Ferguson C;Webb R;Schwudke D;Leneva N;Cho KJ;Cooper L;Rae J;Floetenmeyer M;Oorschot VM J;Skoglund U;Simons K;Hancock JF;Parton RG, 2012, 'Constitutive formation of caveolae in a bacterium', Cell, vol. 150, pp. 752 - 763, http://dx.doi.org/10.1016/j.cell.2012.06.042
Fairn GD;Schieber NL;Ariotti N;Murphy S;Kuerschner L;Webb RI;Grinstein S;Parton RG, 2011, 'High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine', Journal of Cell Biology, vol. 194, pp. 257 - 275, http://dx.doi.org/10.1083/jcb.201012028
Ariotti N;Liang H;Xu Y;Zhang Y;Yonekubo Y;Inder K;Du G;Parton RG;Hancock JF;Plowman SJ, 2010, 'Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formation', Molecular and Cellular Biology, vol. 30, pp. 3795 - 3804, http://dx.doi.org/10.1128/MCB.01615-09
Abankwa D;Hanzal-Bayer M;Ariotti N;Plowman SJ;Gorfe AA;Parton RG;McCammon JA;Hancock JF, 2008, 'A novel switch region regulates H-ras membrane orientation and signal output', EMBO Journal, vol. 27, pp. 727 - 735, http://dx.doi.org/10.1038/emboj.2008.10
Shalom-Feuerstein R;Plowman SJ;Rotblat B;Ariotti N;Tian T;Hancock JF;Kloog Y, 2008, 'K-Ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3', Cancer Research, vol. 68, pp. 6608 - 6616, http://dx.doi.org/10.1158/0008-5472.CAN-08-1117
Plowman SJ;Ariotti N;Goodall A;Parton RG;Hancock JF, 2008, 'Electrostatic interactions positively regulate K-ras nanocluster formation and function', Molecular and Cellular Biology, vol. 28, pp. 4377 - 4385, http://dx.doi.org/10.1128/MCB.00050-08
View less

Contact

+61-2-9385-7397