Dr Nicholas Ariotti

Image of Nicholas Ariotti

Associate Director, EMU

Category: Academic Staff
Research profile: Click to view


Dr Ariotti began his research career at the University of Queensland, Institute for Molecular Biosciences where he completed a PhD in Cell and Membrane Biology in 2013. Dr Ariotti develops novel correlative light and electron microscopy techniques to understand how proteins and lipids are organised in disease. He utilises these techniques to understand the structure, function and composition plasma membrane microdomains at the cell surface.  In 2017, he took up a role as the Associate Director in Biological Electron Microscopy at the Electron Microscope Unit at The University of New South Wales. He has published many peer reviewed articles in journals including Nature, Cell, Nature Cell Biology, Nature Nanotechnology, Current Biology, The Journal of Cell Biology and Developmental Cell.


Conference Papers

McGhee J; Bailey B; Parton RG; Ariotti N; Johnston A, 2016, 'Journey to the Centre of the cell (JTCC): A 3D VR experience derived from migratory breast cancer cell image data', in SA 2016 - SIGGRAPH ASIA 2016 VR Showcase, http://dx.doi.org/10.1145/2996376.2996385

Journal articles

Rae J; Ferguson C; Ariotti N; Webb RI; Cheng H-H; Mead JL; Riches JD; Hunter DJ; Martel N; Baltos J; Christopoulos A; Bryce NS; Cagigas ML; Fonseka S; Sayre ME; Hardeman EC; Gunning PW; Gambin Y; Hall TE; Parton RG, 2021, 'A robust method for particulate detection of a genetic tag for 3D electron microscopy.', Elife, vol. 10, http://dx.doi.org/10.7554/eLife.64630
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Cui Y; Yang Z; Flores-Rodriguez N; Follett J; Ariotti N; Wall AA; Parton RG; Teasdale RD, 2021, 'Formation of retromer transport carriers is disrupted by the Parkinson disease-linked Vps35 D620N variant', Traffic, vol. 22, pp. 123 - 136, http://dx.doi.org/10.1111/tra.12779
Tillu VA; Rae J; Gao Y; Ariotti N; Floetenmeyer M; Kovtun O; McMahon KA; Chaudhary N; Parton RG; Collins BM, 2021, 'Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation', Nature Communications, vol. 12, pp. 931, http://dx.doi.org/10.1038/s41467-021-21035-4
Zhou Y; Ariotti N; Rae J; Liang H; Tillu V; Tee S; Bastiani M; Bademosi AT; Collins BM; Meunier FA; Hancock JF; Parton RG, 2021, 'Caveolin-1 and cavin1 act synergistically to generate a unique lipid environment in caveolae', Journal of Cell Biology, vol. 220, http://dx.doi.org/10.1083/JCB.202005138
Lo H; Lim Y-W; Xiong Z; Martel N; Ferguson C; Ariotti N; Giacomotto J; Rae J; Floetenmeyer M; Moradi SV; Gao Y; Tillu V; Xia D; Wang H; Rahnama S; Nixon S; Bastiani M; Day R; Smith K; Palpant N; Johnston W; Alexandrov K; Collins B; Hall T; Parton R, 2021, 'Muscle-specific Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle', , http://dx.doi.org/10.1101/2021.01.13.426456
Alzahrani HAH; Buckingham MA; Wardley WP; Tilley RD; Ariotti N; Aldous L, 2020, 'Gold nanoparticles immobilised in a superabsorbent hydrogel matrix: Facile synthesis and application for the catalytic reduction of toxic compounds', Chemical Communications, vol. 56, pp. 1263 - 1266, http://dx.doi.org/10.1039/c9cc07046j
Lau D; Walsh JC; Mousapasandi A; Ariotti N; Shah VB; Turville S; Jacques DA; Böcking T, 2020, 'Self-Assembly of Fluorescent HIV Capsid Spheres for Detection of Capsid Binders', Langmuir, vol. 36, pp. 3624 - 3632, http://dx.doi.org/10.1021/acs.langmuir.0c00103
Hall TE; Martel N; Ariotti N; Xiong Z; Lo HP; Ferguson C; Rae J; Lim YW; Parton RG, 2020, 'In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation', Nature Communications, vol. 11, http://dx.doi.org/10.1038/s41467-020-17486-w
Ariotti N; Wu Y; Okano S; Gambin Y; Follett J; Rae J; Ferguson C; Teasdale RD; Alexandrov K; Meunier FA; Hill MM; Parton RG, 2020, 'An inverted CAV1 (caveolin 1) topology defines novel autophagy-dependent exosome secretion from prostate cancer cells', Autophagy, pp. 1 - 17, http://dx.doi.org/10.1080/15548627.2020.1820787
Parton RG; Kozlov MM; Ariotti N, 2020, 'Caveolae and lipid sorting: Shaping the cellular response to stress', Journal of Cell Biology, vol. 219, http://dx.doi.org/10.1083/JCB.201905071
Rae J; Ferguson C; Ariotti N; Webb R; Cheng H-H; Mead J; Riches J; Hunter DJB; Martel N; Baltos J; Christopoulos A; Bryce N; Cagigas ML; Fonseka S; Hardeman E; Gunning P; Gambin Y; Hall T; Parton R, 2020, 'APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy', , http://dx.doi.org/10.1101/2020.10.06.328831
Cui Y; Carosi JM; Yang Z; Ariotti N; Kerr MC; Parton RG; Sargeant TJ; Teasdale RD, 2019, 'Retromer has a selective function in cargo sorting via endosome transport carriers', Journal of Cell Biology, vol. 218, pp. 615 - 631, http://dx.doi.org/10.1083/jcb.201806153
Alshawawreh F; Lisi F; Ariotti N; Bakthavathsalam P; Benedetti T; Tilley RD; Gooding JJ, 2019, 'The use of a personal glucose meter for detecting procalcitonin through glucose encapsulated within liposomes', Analyst, vol. 144, pp. 6225 - 6230, http://dx.doi.org/10.1039/c9an01519a
Meiring JCM; Bryce NS; Lastra Cagigas M; Benda A; Whan RM; Ariotti N; Parton RG; Stear JH; Hardeman EC; Gunning PW, 2019, 'Colocation of Tpm3.1 and myosin IIa heads defines a discrete subdomain in stress fibres', Journal of cell science, vol. 132, http://dx.doi.org/10.1242/jcs.228916
Redpath GMI; Ecker M; Kapoor-Kaushik N; Vartoukian H; Carnell M; Kempe D; Biro M; Ariotti N; Rossy J, 2019, 'Flotillins promote T cell receptor sorting through a fast Rab5–Rab11 endocytic recycling axis', Nature Communications, vol. 10, http://dx.doi.org/10.1038/s41467-019-12352-w
Echarri A; Pavón DM; Sánchez S; García-García M; Calvo E; Huerta-López C; Velázquez-Carreras D; Viaris de Lesegno C; Ariotti N; Lázaro-Carrillo A; Strippoli R; De Sancho D; Alegre-Cebollada J; Lamaze C; Parton RG; Del Pozo MA, 2019, 'An Abl-FBP17 mechanosensing system couples local plasma membrane curvature and stress fiber remodeling during mechanoadaptation', Nature Communications, vol. 10, http://dx.doi.org/10.1038/s41467-019-13782-2
Bayat N; McOrist N; Ariotti N; Lai M; Sia KCS; Li Y; Grace JL; Quinn JF; Whittaker MR; Kavallaris M; Davis TP; Lock RB, 2019, 'Thiol-reactive star polymers functionalized with short ethoxy-containing moieties exhibit enhanced uptake in acute lymphoblastic leukemia cells', International Journal of Nanomedicine, vol. 14, pp. 9795 - 9808, http://dx.doi.org/10.2147/IJN.S220326
Ariotti N; Golani G; Parton R; Michael K, 2019, 'Membrane Curvature and Tension Control the Formation and Collapse of Caveolar Superstructures', Developmental Cell, vol. 48, pp. 523 - 538.e4, http://dx.doi.org/10.1016/j.devcel.2018.12.005
Tavallaie R; McCarroll J; Le Grand M; Ariotti N; Schuhmann W; Bakker E; Tilley RD; Hibbert DB; Kavallaris M; Gooding JJ, 2018, 'Nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood', Nature Nanotechnology, vol. 13, pp. 1066 - 1071, http://dx.doi.org/10.1038/s41565-018-0232-x
Kovtun O; Leneva N; Bykov YS; Ariotti N; Teasdale RD; Schaffer M; Engel BD; Owen DJ; Briggs JAG; Collins BM, 2018, 'Structure of the membrane-assembled retromer coat determined by cryo-electron tomography', Nature, vol. 561, pp. 561 - 564, http://dx.doi.org/10.1038/s41586-018-0526-z
Jung WR; Sierecki E; Bastiani M; O'Carroll A; Alexandrov K; Rae J; Johnston W; Hunter DJB; Ferguson C; Gambin Y; Ariotti N; Parton RG, 2018, 'Cell-free formation and interactome analysis of caveolae', Journal of Cell Biology, vol. 217, pp. 2141 - 2165, http://dx.doi.org/10.1083/jcb.201707004
Ariotti N; Rae J; Giles N; Martel N; Sierecki E; Gambin Y; Hall TE; Parton RG, 2018, 'Ultrastructural localisation of protein interactions using conditionally stable nanobodies', PLoS Biology, vol. 16, http://dx.doi.org/10.1371/journal.pbio.2005473
Forgam H; Esser L; Quinn JF; Mansfeld F; Slapetova I; Whan R; Ariotti N; Davis TP; Kavallaris M; McCarroll J, 2018, 'PO-354 Star-shaped gene silencing nanoparticles– an advanced approach to treating medulloblastoma', ESMO Open, vol. 3, pp. A367 - A368, http://dx.doi.org/10.1136/esmoopen-2018-eacr25.866
Mehmood R; Ariotti N; Yang JL; Koshy P; Sorrell CC, 2018, 'PH-Responsive Morphology-Controlled Redox Behavior and Cellular Uptake of Nanoceria in Fibrosarcoma', ACS Biomaterials Science and Engineering, vol. 4, pp. 1064 - 1072, http://dx.doi.org/10.1021/acsbiomaterials.7b00806
Johnston APR; Rae J; Ariotti N; Bailey B; Lilja A; Webb R; Ferguson C; Maher S; Davis TP; Webb RI; McGhee J; Parton RG, 2018, 'Journey to the centre of the cell: Virtual reality immersion into scientific data', Traffic, vol. 19, pp. 105 - 110, http://dx.doi.org/10.1111/tra.12538
Zhang Y; Ingham B; Cheong S; Ariotti N; Tilley RD; Naffa R; Holmes G; Clarke DJ; Prabakar S, 2018, 'Real-Time Synchrotron Small-Angle X-ray Scattering Studies of Collagen Structure during Leather Processing', Industrial and Engineering Chemistry Research, vol. 57, pp. 63 - 69, http://dx.doi.org/10.1021/acs.iecr.7b03860
Hall TE; Rae J; Ariotti N; Gparton R, 2018, 'Electron Microscopic Detection of Proteins and Protein Complexes in Mammalian Cells Using APEX-tagged, Conditionally Stable Nanobodies', BIO-PROTOCOL, vol. 8, http://dx.doi.org/10.21769/BioProtoc.3088
Ariotti N; Hall TE; Parton RG, 2017, 'Correlative light and electron microscopic detection of GFP-labeled proteins using modular APEX', Methods in Cell Biology, vol. 140, pp. 105 - 121, http://dx.doi.org/10.1016/bs.mcb.2017.03.002
Follett J; Bugarcic A; Yang Z; Ariotti N; Norwood SJ; Collins BM; Parton RG; Teasdale RD, 2016, 'Parkinson disease-linked Vps35 R524W mutation impairs the endosomal association of retromer and induces α-synuclein aggregation', Journal of Biological Chemistry, vol. 291, pp. 18283 - 18298, http://dx.doi.org/10.1074/jbc.M115.703157
Sierecki E; Giles N; Bowden Q; Polinkovsky ME; Steinbeck J; Arrioti N; Rahman D; Bhumkar A; Nicovich PR; Ross I; Parton RG; Böcking T; Gambin Y, 2016, 'Nanomolar oligomerization and selective co-aggregation of α-synuclein pathogenic mutants revealed by single-molecule fluorescence', Scientific Reports, vol. 6, http://dx.doi.org/10.1038/srep37630
Blaževitš O; Mideksa YG; Šolman M; Ligabue A; Ariotti N; Nakhaeizadeh H; Fansa EK; Papageorgiou AC; Wittinghofer A; Ahmadian MR; Abankwa D, 2016, 'Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering', Scientific Reports, vol. 6, http://dx.doi.org/10.1038/srep24165
Hall TE; Ariotti N; Ferguson C; Xiong Z; Parton RG, 2016, 'New Transgenic Lines for Localization of GFP-Tagged Proteins by Electron Microscopy', Zebrafish, vol. 13, pp. 232 - 233, http://dx.doi.org/10.1089/zeb.2016.29002.hal
Ariotti N; Rae J; Leneva N; Ferguson C; Loo D; Okano S; Hill MM; Walser P; Collins BM; Parton RG, 2015, 'Molecular characterization of caveolin-induced membrane curvature', Journal of Biological Chemistry, vol. 290, pp. 24875 - 24890, http://dx.doi.org/10.1074/jbc.M115.644336
Kovtun O; Tillu VA; Ariotti N; Parton RG; Collins BM, 2015, 'Cavin family proteins and the assembly of caveolae', Journal of Cell Science, vol. 128, pp. 1269 - 1278, http://dx.doi.org/10.1242/jcs.167866
Ariotti N; Hall TE; Rae J; Ferguson C; McMahon KA; Martel N; Webb RE; Webb RI; Teasdale RD; Parton RG, 2015, 'Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms', Developmental Cell, vol. 35, pp. 513 - 525, http://dx.doi.org/10.1016/j.devcel.2015.10.016
Zhou Y; Liang H; Rodkey T; Ariotti N; Parton RG; Hancock JF, 2014, 'Signal integration by lipid-mediated spatial cross talk between ras nanoclusters', Molecular and Cellular Biology, vol. 34, pp. 862 - 876, http://dx.doi.org/10.1128/MCB.01227-13
Ariotti N; Fernández-Rojo MA; Zhou Y; Hill MM; Rodkey TL; Inder KL; Tanner LB; Wenk MR; Hancock JF; Parton RG, 2014, 'Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling', Journal of Cell Biology, vol. 204, pp. 777 - 792, http://dx.doi.org/10.1083/jcb.201307055
Lakshminarayan R; Wunder C; Becken U; Howes MT; Benzing C; Arumugam S; Sales S; Ariotti N; Chambon V; Lamaze C; Loew D; Shevchenko A; Gaus K; Parton RG; Johannes L, 2014, 'Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers.', Nature cell biology, vol. 16, pp. 595 - 606, http://dx.doi.org/10.1038/ncb2970
Gambin Y; Ariotti N; McMahon KA; Bastiani M; Sierecki E; Kovtun O; Polinkovsky ME; Magenau A; Jung WR; Okano S; Zhou Y; Leneva N; Mureev S; Johnston W; Gaus K; Hancock JF; Collins BM; Alexandrov K; Parton RG, 2014, 'Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae', eLife, vol. 2014, http://dx.doi.org/10.7554/eLife.01434.001
Kovtun O; Tillu VA; Jung WR; Leneva N; Ariotti N; Chaudhary N; Mandyam RA; Ferguson C; Morgan GP; Johnston WA; Harrop SJ; Alexandrov K; Parton RG; Collins BM, 2014, 'Structural insights into the organization of the cavin membrane coat complex', Developmental Cell, vol. 31, pp. 405 - 419, http://dx.doi.org/10.1016/j.devcel.2014.10.002
Gambin Y; Ariotti N; McMahon KA; Bastiani M; Sierecki E; Kovtun O; Polinkovsky ME; Magenau A; Jung W; Okano S; Zhou Y; Leneva N; Mureev S; Johnston W; Gaus K; Hancock JF; Collins BM; Alexandrov K; Parton RG, 2014, 'Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae', eLife, vol. 3, pp. e01434, http://dx.doi.org/10.7554/eLife.01434
Ariotti N; Parton RG, 2013, 'SnapShot: Caveolae, Caveolins, and Cavins', Cell, vol. 154, pp. 704 - 704.e1, http://dx.doi.org/10.1016/j.cell.2013.07.009
Herms A; Bosch M; Ariotti N; Reddy BJN; Fajardo A; Fernández-Vidal A; Alvarez-Guaita A; Fernández-Rojo MA; Rentero C; Tebar F; Enrich C; Geli MI; Parton RG; Gross SP; Pol A, 2013, 'Cell-to-cell heterogeneity in lipid droplets suggests a mechanism to reduce lipotoxicity', Current Biology, vol. 23, pp. 1489 - 1496, http://dx.doi.org/10.1016/j.cub.2013.06.032
Walser PJ; Ariotti N; Howes M; Ferguson C; Webb R; Schwudke D; Leneva N; Cho KJ; Cooper L; Rae J; Floetenmeyer M; Oorschot VMJ; Skoglund U; Simons K; Hancock JF; Parton RG, 2012, 'Constitutive formation of caveolae in a bacterium', Cell, vol. 150, pp. 752 - 763, http://dx.doi.org/10.1016/j.cell.2012.06.042
Ariotti N; Murphy S; Hamilton NA; Wu L; Green K; Schieber NL; Li P; Martin S; Parton RG, 2012, 'Postlipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes', Molecular Biology of the Cell, vol. 23, pp. 1826 - 1837, http://dx.doi.org/10.1091/mbc.E11-10-0847
Köhnke M; Schmitt S; Ariotti N; Piggott AM; Parton RG; Lacey E; Capon RJ; Alexandrov K; Abankwa D, 2012, 'Design and application of in vivo FRET biosensors to identify protein prenylation and nanoclustering inhibitors', Chemistry and Biology, vol. 19, pp. 866 - 874, http://dx.doi.org/10.1016/j.chembiol.2012.05.019
Fairn GD; Schieber NL; Ariotti N; Murphy S; Kuerschner L; Webb RI; Grinstein S; Parton RG, 2011, 'High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine', Journal of Cell Biology, vol. 194, pp. 257 - 275, http://dx.doi.org/10.1083/jcb.201012028
Ariotti N; Liang H; Xu Y; Zhang Y; Yonekubo Y; Inder K; Du G; Parton RG; Hancock JF; Plowman SJ, 2010, 'Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formation', Molecular and Cellular Biology, vol. 30, pp. 3795 - 3804, http://dx.doi.org/10.1128/MCB.01615-09
Shalom-Feuerstein R; Plowman SJ; Rotblat B; Ariotti N; Tian T; Hancock JF; Kloog Y, 2008, 'K-Ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3', Cancer Research, vol. 68, pp. 6608 - 6616, http://dx.doi.org/10.1158/0008-5472.CAN-08-1117
Plowman SJ; Ariotti N; Goodall A; Parton RG; Hancock JF, 2008, 'Electrostatic interactions positively regulate K-ras nanocluster formation and function', Molecular and Cellular Biology, vol. 28, pp. 4377 - 4385, http://dx.doi.org/10.1128/MCB.00050-08
Abankwa D; Hanzal-Bayer M; Ariotti N; Plowman SJ; Gorfe AA; Parton RG; McCammon JA; Hancock JF, 2008, 'A novel switch region regulates H-ras membrane orientation and signal output', EMBO Journal, vol. 27, pp. 727 - 735, http://dx.doi.org/10.1038/emboj.2008.10
Redpath GMI; Deo N; Siddiqui H; Madani G; Kapoor-Kaushik N; Ariotti N; Rutledge M; Williams M; McCormick SPA, 'Antidepressants are complex regulators of lipoprotein(a) macropinocytosis', , http://dx.doi.org/10.1101/2021.03.26.437114
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Room B87, Basement, Chemical Sciences Building (F10)