Dr Nicholas Ariotti

Education

PhD - Cell and Membrane Biology - The University of Queensland (2013)

BSc (Hons) - The University of Queensland (2006)

Role and Interests

My role is to provide training and support for clients in all facets of biological electron microscopy including sample preparation techniques, correlative light and electron microscopy and cryo-electron microscopy.

My research focuses on understanding how proteins are organised within the lipid bilayer of the plasma membrane and how changes in this organisation can result in disease.

Publications

Follett J, Bugarcic A, Yang Z, Ariotti N, Norwood SJ, Collins BM, Parton RG and Teasdale RD (2015). Parkinson’s disease linked Vps35 R524W mutation impairs retromer’s endosomal association and induces α-synuclein inclusion formation. Accepted: The Journal of Biological Chemistry.

Blaževitš O, Mideksa YG, Šolman M, Ligabue A, Ariotti N, Nakhaeizadeh H, Fansa EK, Papageorgiou AC, Wittinghofer A, Ahmadian MR and Abankwa D (2016). Galectin-1 dimers can scaffold Raf-effectors to increase H-ras nanoclustering. Scientific Reports 6, 24165. doi: 10.1038/srep24165

Hall TE*, Ariotti N*, Ferguson C, Xiong Z and Parton RG (2016). New Transgenic lines for localization of GFP-tagged proteins by electron microscopy. Zebrafish 13, 232-3. *Equal contribution.

Ariotti N, Hall TE, Rae J, Ferguson C, Martel N, Webb R, McMahon K, Webb RI, Teasdale RD and Parton RG (2015). Modular detection of GFP-labeled proteins for rapid screening by electron microscopy in cells and animals. Developmental Cell 35, 513-25. doi: 10.1016/j.devcel.2015.10.016

Ariotti N, Rae J, Leneva N, Ferguson C, Loo D, Okano S, Hill MM, Walser PJ, Collins BM and Parton RG (2015). Molecular characterization of caveolin-induced membrane curvature. The Journal of Biological Chemistry 2015 August 24. doi: 10.1074/jbc.M115.644336.

Kovtun O, Tillu VA, Ariotti N, Parton RG and Collins BM (2015). Cavin family proteins and the assembly of caveolae. Journal of Cell Science 123(7), 1269-1278.

Kovtun O, Tillu VA, Jung WR, Leneva N, Ariotti N, Chaudhary N, Mandyam RA, Ferguson C, Morgan GP, Johnston WA, Harrop SJ, Alexandrov K, Parton RG and Collins BM (2014). Structural insights into the organization of the cavin membrane coat complex. Developmental Cell 31, 405-19.

Lakshminarayan R, Wunder C, Becken U, Howes MT, Benzing C, Arumugam S, Sales S, Ariotti N, Chambon V, Lamaze C, Loew D, Shevchenko A, Gaus K, Parton RG and Johannes L. (2014). Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers. Nature Cell Biology 16, 595-606.

Ariotti N, Fernandez-Rojo MA, Zhou Y, Hill MM, Rodkey TL, Inder KI, Tanner LB, Wenk MR, Hancock JF and Parton RG (2014). Caveolae regulate the nanoscale organization of the plasma membrane to remotely control Ras signaling. The Journal of Cell Biology 204, 777-92.

Zhou Y, Liang H, Rodkey T, Ariotti N, Parton RG and Hancock JF (2014). Signal integration by lipid-mediated spatial cross talk between Ras nanoclusters. Molecular and Cellular Biology 34, 862-76.

Ariotti N and Parton RG (2013). Snapshot: Caveolae, caveolins, and cavins. Cell 15, 704-704.

Gambin Y, Ariotti N, McMahon K, Bastiani M, Sierecki E, Kovtun O, Magenau A, Jung W, Okano S, Zhou Y, Mureev S, Johnston W, Gaus K, Hancock JF, Alexandrov K and Parton RG (2013). Single-molecule analysis reveals self-assembly and nanoscale segregation of two distinct subcomplexes on caveolae. eLife 3, e01434.

Herms A, Bosch M, Ariotti N, Reddy BJN, Fajardo A, Fernández-Vidal A, Alvarez-Guaita A, Fernández-Rojo MA, Rentero C, Tebar F, Enrich C, Geli M, Parton RG, Gross SP and Pol A (2013). Cell to cell lipid droplet heterogeneity: a population advantage to reduce lipotoxicity. Current Biology 23, 1489-96.

Walser PJ, Ariotti N, Howes MT, Ferguson C, Webb R, Schwudke D, Leneva N, Cho KJ, Cooper L, Rae J, Floetenmeyer M, Oorschot VM, Skoglund U, Simons K, Hancock JF and Parton RG (2012). Constitutive formation of caveolae in a bacterium. Cell 150, 752-63.

Ariotti N, Murphy S, Hamilton NA, Wu L, Green K, Schieber NL, Li P, Martin S and Parton RG (2012). Post-lipolytic insulin-dependent remodeling of micro lipid droplets in adipocytes. Molecular Biology of the Cell 23, 1826-37.

Köhnke M, Schmitt S, Ariotti N, Piggott AM, Parton RG, Lacey E, Capon RJ, Alexandrov K and Abankwa D (2012). Design and application of in vivo FRET-biosensors to identify protein prenylation and nanoclustering inhibitors. Chemistry & Biology 19, 866-74.

Fairn GD, Schieber NL, Ariotti N, Murphy S, Kuerschner L, Webb RI, Grinstein S and Parton RG (2011). High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine. Journal of Cell Biology 194, 257-75.

Ariotti N, Liang H, Xu Y, Zhang Y, Yonekubo Y, Inder K, Du G, Parton RG, Hancock JF and Plowman SJ (2010). Epidermal growth factor receptor activation remodels the plasma membrane lipid environment to induce nanocluster formation. Molecular and Cellular Biology 30, 3795-804.

Shalom-Feuerstein R, Plowman SJ, Rotblat B, Ariotti N, Tian T, Hancock JF and Kloog Y (2008). K-ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3. Cancer Research 68, 6608-16.

Plowman SJ, Ariotti N, Goodall A, Parton RG and Hancock JF (2008). Electrostatic interactions positively regulate K-Ras nanocluster formation and function. Molecular and Cellular Biology 28, 4377-85.

Abankwa D, Hanzal-Bayer M, Ariotti N, Plowman SJ, Gorfe AA, Parton RG, McCammon JA and Hancock JF (2008). A novel switch region regulates H-ras membrane orientation and signal output. EMBO Journal 27, 727- 35