Structural Biology Facility (SBF)

Circular dichroism (CD) measures the difference in the absorbance of left-handed circularly polarised light (L-CPL) and right-handed circularly polarised light (R-CPL),occuring in molecules with chiral chromophores (light-absorbing groups). it is primarily used to analyse the secondary structure or conformation of macromolecules, particularly proteins. Since secondary structure is sensitive to its environment, e.g. temperature or pH, CD can be used to observe how these structures change under different environmental conditions or when interacting with other molecules.

The Art Robbins Crystal Phoenix is a nano-litre capable protein crystallisation dispenser for high throughput preparation of crystal trays, featuring accuracy, speed, and precision to make it the perfect instrument for sitting drop, hanging drop and micro batch reactions. In less than 50 seconds it dispenses protein and screen to the small wells or hanging drop seals.

The Bruker D8 Venture X-ray diffractometer is a state-of-the-art instrument suitable for protein crystal diffraction. It is fitted with the following features: Photon III detector, Automatic ‘2 click’ AGH Kappa goniometer, Diamond IµS copper source, Oxford Cryo-stream, optional use of the ISX plate goniometer.

The Constant Systems CF1 Cell Disrupter is used for breaking biological samples physically to allow for further sample processing. The unit is typically used for breaking cells during the protein purification process. The instrument can operate in continuous flow mode allowing up to 6L of sample to be lysed within an hour. The instrument enables samples to be lysed at a maximum processing pressure of 40 kpsi (2700 bar). The unit has an integrated cooling jacket and an auto shutdown feature for when process is complete.

The Flashforge Creator Pro 3D printer is a dual head 3D printer used to prepare scientific models of protein structures. The unit has the capability of printing in two colours at a time and has a large print-bed allowing large models to be made.

The SBF has two Formulatrix NT8 liquid handlers for protein crystallisation. One is set up at room temperature and the other is set up at 4˚C. The NT8 units are fast nano-litre volume liquid handlers specifically made for protein crystallisation tray preparation. The units are small and easy to use, with built-in humidity control aand reusable disposable plastic tips for dispensing, ensuring your crystal trays are prepared under the best possible conditions.