The Nikon Eclipse TiE is ideally suited for live cell imaging studies. With one of the best focus stabilisation devices on the market the Perfect Focus System (PFS) ensures that the focus you set at the start of the experiment will be maintained throughout its entirety. They system is fitted with several phase contrast objects making it suitable for imaging cells through a range of materials including plastic, as well as 3 high NA DIC objects for achieving astounding detail if imaging through glass.
This is a total internal fluorescence (TIRF) microscope built on an inverted Nikon Ti2-E microscopeThe TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. It is equipped with piezo Z stage and Nikon Perfect Focus System 4, making it ideal for long term imaging. It is equipped with stage top incubator and objective heater as well as CO2, allowing live cell imaging. With quad filter, 4 solid state laser lines,
The Nikon Eclipse Ti2-E is an inverted widefield system offering a large field of view, high-quality optics and an industry-leading focus stabilisation system (Nikon Perfect Focus System). With a stage insert providing temperature and CO2 control, this system is ideal for live-cell imaging. The system software includes Nikon JOBS, allowing automation of experimental protocols, including on the go analysis and cell identification. In addition to these features, this microscope is connected to a Cetoni liquid-handling unit, allowing for automated liquid exchange. Whether you are performing live-cell experiments, sample screening or automated experimental protocols, this system can perform the task with minimal researcher input.
This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet (HiLo: https://www.nature.com/articles/nmeth1171) modalities in one instrument. The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample. The system is also supplemented with NIDAQ board and control with external trigger, which combined with the versatile illumination sequence option makes this ideal microscope for complex experiments involving the correct timing of imaging following an external stimulus of cells or animal. Definite focus is perfectly controlled using Nikon PFS system making it ideal for long term live cell or animal imaging.
The Microtime200, manufactured by the German-based PicoQuant GmbH, is generally used in conjunction with the TCS SP5 microscope. By using the lasers from the TCS SP5, the Microtime200 is capable of measuring how fast proteins move within the cell (diffusion) and protein to protein interactions.