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Nikon A1 Intravital

Confocal Intravital

The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability.

Nikon Biostation

Florescence Widefield Phase Contrast

Nikon Biostation is an inverted life cell imaging microscope enclosed in an incubator. The stage and objective are fully enclosed in temperature modulated box resulting in no focus drift during imaging. The cells are kept at 37C and supplemented with 5% CO2 throughout the entire experiment. The imaging chamber can hold 35mm glass or plastic bottom dishes of 0.17um thickness. The Biostation is an ideal microscope for imaging cell migrations, wound healing, cell division and cell growth and adherence.
Nikon N-Storm (Ramaciotti)

Super-resolution TIRF Widefield Funded by Ramaciotti Foundation

This is a total internal fluorescence (TIRF) microscope built on an inverted Nikon Ti2-E microscopeThe TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. It is equipped with piezo Z stage and Nikon Perfect Focus System 4, making it ideal for long term imaging. It is equipped with stage top incubator and objective heater as well as CO2, allowing live cell imaging. With quad filter, 4 solid state laser lines,

Nikon Ti2 (Biosciences)

Fluorescence Widefield Phase Contrast DIC Calcium Imaging

The Nikon Eclipse Ti2-E is an inverted widefield system offering a large field of view, high-quality optics and an industry-leading focus stabilisation system (Nikon Perfect Focus System). he system software includes Nikon JOBS, allowing automation of experimental protocols, including on the go analysis and cell identification. Additionally, a CoolLED pE-340Fura light source enables rapid ratiometric imaging of rapid calcium dynamics.

Nikon Ti2 (Ramaciotti)

Fluorescence Widefield High Throughput Phase Contrast DIC Funded by Ramaciotti Foundation

The Nikon Eclipse Ti2-E is an inverted widefield system offering a large field of view, high-quality optics and an industry-leading focus stabilisation system (Nikon Perfect Focus System). With a stage insert providing temperature and CO2 control, this system is ideal for live-cell imaging. The system software includes Nikon JOBS, allowing automation of experimental protocols, including on the go analysis and cell identification. In addition to these features, this microscope is connected to a Cetoni liquid-handling unit, allowing for automated liquid exchange. Whether you are performing live-cell experiments, sample screening or automated experimental protocols, this system can perform the task with minimal researcher input.

Nikon TIRF/HiLo

Florescence TIRF HiLo Intravital Widefield

This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet modalities in one instrument. TIRF modality allows user to image molecules at the surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample.

Operetta CLS

Fluorescence High Throughput Spinning Disk Confocal

The Operetta CLS is a multi-modal high-content microscopy system. Designed for high throughput, it can capture both widefield and spinning disk confocal images. Setup a single well in a 96 well plate and simply allow the system to replicate those settings across the entire plate. With an automated water immersion objective you also don’t have to sacrifice resolution for high throughput multipoint positioning.

Phasefocus LiveCyte

Ptychography

The Phasefocus Livecyte is an inverted system for live cell imaging. This microscope has Quantitative Phase Imaging (QPI, also known as Ptychography) a label-free approach that utilises relative phase shift information to produces images of cells and tissues with high contrast and high fidelity with low phototoxicity and photobleaching. The system is also equipped with widefield fluorescence capabilities for researchers that wishes to identify cells and cellular components with high specificity.

Picoquant Microtime 200

Confocal FLIM

The Microtime200, manufactured by the German-based PicoQuant GmbH, is generally used in conjunction with the TCS SP5 microscope. By using the lasers from the TCS SP5, the Microtime200 is capable of measuring how fast proteins move within the cell (diffusion) and protein to protein interactions.

PicoQuant Microtime 200 rapidFLIM STED

Confocal Rapid FLIM Super-resolution Funded by Cancer Institute NSW

Fluorescence Lifetime Imaging (FLIM) adds another dimension to conventional intensity based imaging methods. FLIM is not affected by fluctuations in the fluorescence intensity and permits to discrimination between fluorophores with similar emission spectra (and from auto-fluorescence. It can be used to probe local environmental conditions (e.g., pH value), to determine ion concentrations, to study intracellular signal transduction or to distinguish between different tissue components.

Precision X-ray X-Rad 320 Biological Irradiator

The X-Rad 320 is a self contained biological irradiator for in vivo and in vitro applications.