Picoquant Microtime200

 
The Microtime200, manufactured by the German-based PicoQuant GmbH, is generally used in conjunction with the TCS SP5 microscope. By using the lasers from the TCS SP5, the Microtime200 is capable of measuring how fast proteins move within the cell (diffusion) and protein to protein interactions. The MicroTime200 is a time-resolved, inverted, confocal fluorescence microscope with single-molecule sensitivity for fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging (FLIM).
 
icroscope body:
Inverted
Motroised Stage:
No
Temp Control:
Stage insert
CO2:
No
Software:
SymPhoTime 64

 

Common Applications


 

FLIM: Each fluorescent dye displays a characteristic fluorescence lifetime, namely the length of time following excitation that the dye will remain in its excited state emitting fluorescence.  This fluorescence lifetime is a quantitative signature that can be used to distinguish between different fluorescent dyes, even those emitting the same fluorescent colour.
FCS: Fluorescence correlating spectroscopy allows for determining diffusion coefficients and concentrations of fluorescently labelled molecules at nanomolar concentrations both in vitro and in live cells. It is mostly useful for indirect studies of molecular activity in plasma membrane, in cytosol and in nucleus via following relative changes in diffusive behaviour and/or concentration.

 

Objective Lenses

 

MAG N.A CORRECTIONS IMMERSION W.D MISC.
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Light Sources

Filters

Detectors