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The 3i VIVO microscope is ideally suited for intravital imaging. Its large stable stage can easily accommodate sizable tissue samples along with clamps and manipulators to aid stabilization. Equipped with a spinning disk unit for high speed optical sectioning, paired with an image intensifier, this system can achieve very high frame rates making the most of every photon. Additionally, a pulsed 532nm ablation laser is available for targeted tissue damage.
Quadrupole mass filters provide a robust means of sorting ions in a mass spectrometry experiment. When these mass analysers are incorporated in a triple quadrupole (QQQ) setup, which consists of three quadrupole mass filters in series, various specialised forms of quantitative and qualitative mass spectrometry can be conducted. This is achieved via the use of the first and third quadrupoles (Q1 and Q3, respectively) as mass filters, whilst the second quadrupole (Q2) is utilised as a collision cell in which precursor ions undergo collision-induced dissociation to produce fragment ions.
The Agilent 7890 is one of the high performance gas chromatographs that provide superior performance for all applications. The key to its performance is the use of advanced EPC (electronic pneumatic control) modules and high performance GC oven temperature control which allows for fast and precise temperature ramping. These specifications provide optimal chromatography including peak symmetry, repeatability of retention time and accuracy in retention time index. Agilent 7890A is expected to have retention time repeatability <0.008% or <0.0008min and area repeatability <1%RSD.
Gas Chromatography-mass spectrometry (GC-MS) combines two powerful analytical tools: gas chromatography for the highly efficient gas-phase separation of components in complex mixtures, and mass spectrometry for the confirmation of identity of these components as well as for the identification of unknowns. The HP 6890-5973 GC/MSD allows the analysis of small molecules (m/z 50-800) in a wide range of sample matrices. This system can be operated in electron ionisation (EI) and chemical ionisation (CI) modes.
The HP 1090 HPLC system is useful for a wide range of applications for metabolites, peptides and proteins including: sample clean up; enrichment, and fractionation. With flow rates of between 50–5000 μl/min it suits elution using narrow bore to analytical size columns that can be detected with a UV detector. Columns can be temperature controlled and there are also 3 solvent reservoirs for solvent delivery binary, ternary or gradient elution. The application of this instrument to many kinds of separation columns is the advantage of this stand alone LC system.
The GE AKTA Purifier LC system and fraction collector is used predominantly for protein purification, as it has no metal parts. The system has a manual injector, with 3 channel UV and conductivity detectors. The Solvent delivery system is capable of delivering both isocratic and gradient elution, and can be used for both analytical and semi-preparative work and fractionation depending on choice of column used. Major work projects have included the purification and fractionation of cytochrome C and related products.
ABI QStar Elite is a high-performance, hybrid quadrupole Time-of-Flight (TOF) mass spectrometer typically used with electrospray with greater sensitivity and intelligent dependent scan functions. The system is used for protein or peptide characterisation using nano liquid chromatography and data dependent tandem mass spectrometry. The system is currently used for iTRAQ quantification of differentially expressed proteins and these normally combine online multi-dimensional liquid chromatography.
BD FACSCanto™ II is equipped with three excitation lasers wavelengths (405nm, 488nm and 640 nm). The Canto II cant detect up to 10 fluorescent parameters with 2 scatter options and is capable of analyzing samples between 100 and 12,000 cells per second. Software is FACS™ Diva generating standard FCS3.0 files for third party software analysis packages.
The BD FACSVerse™ is equipped with three excitation lasers (wavelengths 405nm, 488nm and 640 nm). The Verse can detect up to 8 fluorescent parameters with 2 scatter options. Sample uptake on this instrument is via suction, thus enabling experiments such as drug uptake. Software is FACSuite™ generating standard FCS3.0 files for third party software analysis packages.
The BD Influx™ cell sorter is equipped with five excitation lasers (ultraviolet 355nm, violet 405nm, blue 488nm, yellow/green 561nm and red 642nm) and has a flexible detection configuration with a number of filter set options, detecting up to 24 parameters (21 fluorescence channels, two forward scatter channels and one side scatter).
The BD LSRFortessa™ SORP analyzing flow cytometer is a high-end, user-friendly flow cytometer with 5 excitation lasers and 24 detection parameters (matching our BD Influx’s configuration). Optimised for both biomedical and environmental research, this cytometer offers an optional High Throughput Sampling system, and small particle detection.
The BD LSRFortessa™ SORP X-20 analyzing flow cytometer is equipped with five excitation lasers (ultraviolet 355nm, violet 405nm, blue 488nm, yellow/green 561nm and red 642nm) and is able to detect up to 20 parameters (18 fluorescence channels plus forward scatter and side scatter). This cytometer’s configuration has been designed to mirror that of our BD Influx (excluding small particle detection), enabling users to easily transfer or work-up experiments.
The high throughput sampling (HTS) is attached to the LSRFortessa allowing users to analyse sample directly from a plate (12, 24, 96 and 384 wells).