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Thermo MAT 253 IRMS with KIEL

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Thermo Orbitrap Fusion Lumos

Thermo Orbitrap Fusion Lumos

The Orbitrap Fusion Lumos includes an exclusive Tribrid design incorporating 3 different mass analysers (a quadrupole, an ion trap and an Orbitrap) enabling a large number of different experiments to be performed with a single mass spectrometer.  Software developments have also simplified the set-up of complex experiments.  Other significant technological advances including a brighter ion source and improved vacuum/pumping.  Additional enhancements of the Lumos include: greater scan speed, high mass resolving power, sensitivity and an improved ETD capability and EThcD fragm

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Thermo QExactive Plus

QExactive mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements in both MS and MS/MS experiments; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The QE+ is a hybrid instrument that incorporates both quadrupole and Orbitrap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument.

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Thermo Trace DSQ

The Thermo DSQ II GC-MS instruments are our most up-to-date instruments for analysis of compounds that are sufficiently volatile to enter the gas phase intact. The Mass Spectrometer of the Trace DSQ II GC-MS instrument at our Upper Campus facility can be operated in either electron impact (EI+) positive ionization mode, positive ion chemical ionization (CI+) mode, or electron capture negative ionization mode (ECNI or CI-). The Quadrupole MS gives nominal m/z assignments, accurate to +/- 0.2 Da.

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Thermo TSQ Access

The TSQ Quantum is a high performance triple stage quadrupole mass spectrometer. It includes a syringe pump, a divert/inject valve, an atmospheric pressure ionisation (API) source, and the Xcalibur™ data system. You can operate the mass spectrometer in either electrospray ionisation (ESI) mode or atmospheric pressure chemical ionisation (APCI) mode. Typically, polar compounds such as amines, peptides, and proteins are best analysed by ESI, and non-polar compounds such as steroids are best analysed by APCI.

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Thermo TSQ XLS

The TSQ XLS is a triple quadrpole mass spectrometer and it is equipped with gas chromatographic sample separation.  The instrument is capable of Electron Impact and Chemical Ionization modes and can operate in either positive and negative ion polarities.  The instrument is used predominately for small molecule quantitiave analysis.

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Thin Film Analyser - Mikropack NanoCalc 2000 UV-Vis-NIR

The instrument is a UV-visible-NIR thin film analyser which can provide a measurement of the thickness of multilayer films. The instrument measures light reflected perpendicular to a sample surface which can provide information on the thickness of up to 4 layers in a sample, and is useful for an independent comparison with film-thickness measurements obtained on the MRD. The wavelengths available range from 250nm to 850nm, which enable measurement of film thickness from 10nm up to 20micron. This instrument is located in lab G65, and it available at no charge to UNSW users.

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TILL Photonics iMic TIRF System

TILL Photonics iMic TIRF System

This microscope has a unique design, allowing for a great deal of customization of the sample space and experimental design. The system is designed for up to four-color total internal fluorescence (TIRF), photoactivation (PA), fluorescence resonance energy transfer (FRET), widefield correlation spectroscopy (WCS), spatio-temporal image correlation spectroscopy (STICS), and fluorescence recovery after photobleaching (FRAP) experiments, all at high frame rates. Single-molecule measurements can be enhanced with a pair of single-photon avalanche photodiodes (APDs) and a pulse correlator for fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) experiments. An environmental chamber allows for these techniques to be extended to live cell samples.

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Time of Flight Secondary Ion Mass Spectrometer (TOFSIMS)

The ION-TOF TOF.SIMS 5  was installed in to our lab in May 2015. The instrument is located in F10:G61. Contact Dr Bill Gong (b.gong@unsw.edu.au) for more details on its capabilities.

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VisualSonics Vevo 2100 Ultrasound

VisualSonics Vevo 2100 Ultrasound

The VisualSonics Vevo2100 uses the new solid-state transducers which offer higher resolution at multiple focal depths. The system offers superior resolution down to 30μm, a wide range of frequencies (13-56 MHz) and a large field of view. Importantly, the Vevo2100 offers a comprehensive imaging and processing packing.

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Waters/Micromass Q-TOF Ultima

The Waters Q-TOF Ultima is a quadrupole, orthogonal acceleration time-of-flight tandem mass spectrometer. It equipped with ESI and nanoESI interfaces. It has high resolution, sensitivity and mass accuracy. The Q-TOF Ultima is mainly used for LC-coupled MSMS analysis of peptides derived from gel spots and bands, accurate mass determination of biomolecules, protein post-translational modification studies, e.g. glycopeptide mapping.

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XRD Experiments Using Variable Temperature or Controlled Atmophere

The diffraction instruments have a range of accessories for dynamic monitoring of phase changes from 10-1800 Kelvin, in certain cases using a controlled atmosphere.

Bruker D8 with rotating anode copper source:

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Zeiss Axio Observer X.1 SD & TIRF

Zeiss Axio Observer X.1 SD & TIRF

The zeiss Spinning Disk/TIRF system is a custom built instrument.  Built upon an Axio Observer X.1 body it is equipped with a Yokogawa Spinning disk CSU-X1 module providing confocal imaging at rapid frame rates captured on an EM CCD camera (Quant EM) ideally suited for low light imaging. Attached to a different microscope port is a highly sensitive high frame rate sCMOS camera (Orca Flash 4) which can be used for standard epifluorescence, rapid ratiometric imaging utilising a fast excitation switching light source (Lambda DG4) or acquire fluorescence images using TIRF illumination.

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Zeiss Elyra PALM/SIMS Superresolution

Zeiss Elyra PALM/SIMS Superresolution

The Zeiss Elyra Superresolution Microscope is a developmental superresolution microscope with capabilities to push imaging past the classic diffraction limit. Typically visible light microscopes cannot achieve spatial resolution of greater than 200-250 nanometers. This system has two different imaging modalities to push this limit as low as 20 nanometers.

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Zeiss Lightsheet Z.1

Zeiss Lightsheet Z.1

Lighsheet microscopy is a fluorescence-based imaging technique that is growing in popularity. It outperforms other techniques in its ability to rapidly capture large optically sectioned three dimensional datasets, whilst keeping photo-toxicity and bleaching to a minimum. It therefore lends itself well to imaging samples larger than those typically attempted by conventional fluorescence microscopy such as tissues, whole organs and even embryo development. 

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Zeiss LSM 780

Zeiss LSM 780

The Zeiss LSM 780 is a high-sensitivity laser scanning confocal microscope. Unlike most standard confocal systems this instrument comes equipped with a GaAsP photodetector. This detector in addition to boasting a quantum efficiency of 45% (in comparison to 25% for standard PMTs), it can also be used as a single-point detector or as a 32-channel detector array facilitating spectral measurements. It can also be switched into a single-photon counting mode providing great signal to noise for such techniques as FCS and RICS. 

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Zeiss LSM 880

Zeiss LSM 880

The Zeiss LSM 880 is an inverted laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector and 2 multialkali PMTs, which can be used as single point detectors or combined for 34 channel spectral imaging. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (140nm axially, 400 nm laterally) and 4-8 x greater signal-to-noise ratio, at the highest speed of any linear scanning confocal system. BiG.2 Type A (2x GaAsP detectors) non-descanned detectors for the multi-photon laser can be used for imaging thick specimens, with deeper penetration and decreased phototoxicity compared to single photon lasers. The Picoquant system utilises the multi-photon laser to perform Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Forster Resonance Energy Transfer (FRET). The Zeiss 880 is also equipped with polarisers for anisotropy measurements. 

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Zetasizer Nano ZS

Zetasizer Nano ZS

The Zetasizer Nano ZS is a high performance two angle particle and molecular size analyser for the enhanced detection of aggregates and measurement of small or dilute samples, and samples at very low or high concentration using dynamic light scattering with ‘NIBS’ optics. The ZSP also incorporates a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular weight analyzer using static light scattering.

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