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Lavision TriM Scope II Multiphoton Intravital

Lavision TriM Scope II Multiphoton Intravital

This intravital microscope boasts an impressive collection of hardware. Equipped with 2 multiphoton lasers utilising a pre-chirp compensator to keep laser pulse width < 150 fs, a collection of detectors including high sensitive NDD detectors close to the objective lens, descanned PMTs, imaging cameras for fast 64 beam scanning and also LaVision BioTec's FLIM x16 TCSPC detector for rappid fluorescence lifetime measurements. 

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LECO High Temperature NO Elemental Combustion Analysis Unit

The LECO NO instrument was purchased in 2010. This is normally a service only instrument, but user training is possible if you contact the lab. The instrument is designed for samples requiring very high temperatures to decompose, eg. metals, ceramics.

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LECO TruSpec Analyser: CN Module and S Module

The LECO CN and S instruments were purchased in 2007. They provide rapid, fully automated rapid analyses of organic and inorganic macrosamples (~100 mg) to determine carbon, nitrogen and sulphur.

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Leica 165C Microscope

The Leica microscope has a large working distance between the objective and sample and an incident cold light source to enable our crystallographers to mount single-crystals onto a capillary, prior to measuring the diffraction pattern. Images and dimensions of a crystal can be collected using the CCD camera. When not in use by our crystallographers, the microscope may be used, at no cost, by UNSW researchers who require a low to medium magnification (max.90x) digital image of their sample.

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Leica DM IL

Leica DM IL

The Leica DM IL microscope is used to illuminate whole cells; observing and analysing a cell as a single entity. The DM IL is an inverted epifluorescence microscope, with transmitted light LED back illumination and an EL6000 Fluoro system.

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Leica TCS SP5 MP STED

Leica TCS SP5 MP STED

Leica TCS SP5 equipped with multiphoton Ti-Sapphire laser is an inverted confocal laser scanning microscope. It is designed for high-resolution fluorescence and transmitted light imaging with single or multi-photon excitation, in 2D, 3D or 4D with live or fixed cells. It can also be used for Fluorescence Recovery After Photo bleaching (FRAP), pulsed Stimulated Emission Depletion (pulsed STED) and Fluorescence Correlation Spectroscopy (FCS).

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Leica TCS SP5 WLL STED

Leica TCS SP5 WLL STED

This Leica TCS SP5 confocal system is equipped for a broad range of experimental requirements. Along with a range of fixed wavelength laser lines it now has a White-Light Laser (WLL) tunable within the range x-x for precise control over fluorophore excitation. Fitted with Hybrid Dectors (HyD) for enhanced sensitivity, and interchangeable Galvano and resonant scanners, this system is suitable for applications requiring both speed and/or improved signal-to-noise. Its Gated-STED module also enables colleciton of super-resolution images allowing investigators to distinguish objects and structures beyond the conventional resolution limit of light.

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Liquid Chromatography

Separation and Fractionation

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Liquid Chromatography / Mass Spectrometry

Proteins, Polymers and other High MW or Polar Molecules including Proteomics

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Matrix Assisted Laser Desorption / Ionisation

Proteins, Polymers and other High MW or Polar Molecules including Proteomics

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Multispectral Optoacoustic Tomography

Multispectral Optoacoustic Tomography

Optoacoustic or photoacoustic imaging is a new, cutting-edge imaging technique which detects ultrasound waves following light absorption from specific molecules.  This technique allows molecules with different spectral profile to be detected.

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Muromachi Microwave Fixation System

Muromachi Microwave Fixation System

The Muromachi microwave fixation system offers an effective way to inactivate these enzymes in the intact specimen.

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NanoSight NS300

NanoSight NS300

The NanoSight NS300 utilizes the latest version of NanoSight’s particle characterization technology, Nanoparticle Tracking Analysis (NTA). This elegant new instrument offers the unique benefits scientists have come to expect from NanoSight in a compact format, with the major benefit of enhanced fluorescence capability.

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Nikon A1 Intravital

Nikon A1 Intravital

The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability. 

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Nikon Biostation

Nikon Biostation

Nikon Biostation is an inverted life cell imaging microscope enclosed in an incubator. The stage and objective are fully enclosed in temperature modulated box resulting in no focus drift during imaging. The cells are kept at 37C and supplemented with 5% CO2 throughout the entire experiment. The imaging chamber can hold 35mm glass or plastic bottom dishes of 0.17um thickness. The Biostation is an ideal microscope for imaging cell migrations, wound healing, cell division and cell growth and adherence.

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Nikon Eclipse Tie

Nikon Eclipse Tie

The Nikon Eclipse TiE is ideally suited for live cell imaging studies. With one of the best focus stabilisation devices on the market the Perfect Focus System (PFS) ensures that the focus you set at the start of the experiment will be maintained throughout its entirety. They system is fitted with several phase contrast objects making it suitable for imaging cells through a range of materials including plastic, as well as 3 high NA DIC objects for achieving astounding detail if imaging through glass.

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NuSep ProteomSep/MF10 Electrophoretic Partitioning Systems

This in-solution electrophoretic device is for the partitioning of μl volumes of μg-mg amounts of proteins and or peptides based on surface charge, pH, size in denaturing or native formats. It is typically used to partition contaminant proteins away from lower abundance proteins (eg. Albumin or Ig from other plasma proteins), desalting of biological samples (eg. urine, plasma, tears), enrichment of poorly concentrated and low abundance samples for further analysis. Partitioning down to 1kDa is achievable and is therefore ideal for peptide and small protein work.

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Off-line Separation

1 dimensional & 2 dimensional gel eletrophoresis (SDS PAGE & 2DE) and in-solution electrophoretic partitioning are important off-line separation methods for large biomolecules such as proteins and nucleic acids. The BMSF has a comprehensive range of separation devices and mass spectrometers to address most analytical problems suited to mass spectrometry.

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Olympus Delta Premium Portable XRF Spectrometer

The Olympus Delta Premium PXRF Spectrometer was purchased in 2014. This is normally a service only instrument, but user training is possible if you contact the lab. The instrument comes with two factory calibrations (Geochem and Soil), as well as calibrations for coal, and can be calibrated to suit a specific sample type.

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PANalytical AXIOS Advanced Simultaneous WDXRF Spectrometer

The PANalytical AXIOS-Advanced WDXRF spectrometer was commissioned in 2010 and is fitted with an SST-MAX X-ray sourcewith a 4kW output and can be operated at 160mA. The resulting high X-ray flux enables high sensitivity and low detection limits for light elements. The AXIOS can measure elements over wide dynamic concentration ranges and include all the elements between Be and U in the periodic table.

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PANalytical Empyrean 2 X-ray Diffraction System (Cobalt)

The Empyrean 2 is a Bragg-Brentano geometry X-ray diffractometer that was installed in 2014 and is equipped with the following components:

  • Cobalt anode X-ray tube to minimise fluorescence from iron-containing samples that occurs when using the standard copper X-ray anode
  • High resolution optic for cobalt X-rays to minimise scatter at low angles
  • Sample changer for large batches 

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PANalytical Empyrean Thin-Film XRD

The Empyrean Thin-Film XRD, instaklled 2013, provides similar capability to the MRD for thin-film analysis.

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PANalytical MINIPAL 4, EDXRF Spectrometer

The MINIPAL was purchased in 2006. It has a 12 position sample changer and is capble of analysing sodium through to uranium. Data can be optionally processed via full fundamental parameter model and Compton ratio correction, and a standardless program for metal analysis is available.

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