Techniques

PROTEOMICS

  • Analysis of tryptic digests, gel-fractions and peptides in biological extracts
  • Determine pancreatic polypeptide levels in human plasma
  • Mass determinations of intact proteins/glysosylations level estimation
  • Top/Middle Down protein analysis by ECD and ETD
  • Development of phosphorylation assays used to investigate cell-signalling
  • Phosphopeptide analysis by TiO2, IMAC and anti-phosphotyrosine enrichment
  • Quantitation of specific peptides in biological fluids/tissues/organism by MRM
  • Low mass peptide/protein (<25kDa) enrichment from complex proteomes
  • nanoLC-MS/MS protein digest analysis (LysC, AspN, Chyotrypsin etc)
  • De novo sequencing of peptides and small proteins
  • Mascot, SEQUEST, MaxQuant or PEAKS searches for protein identification
  • High resolution mass accuracy analysis (including ECD, ETD, IRMPD)
  • iTRAQ analysis and quantitation
  • SILAC analysis and quantitation
  • High resolution UPLC label free protein quantification¬†
  • Static nanospray of peptides and proteins including antibodies


METABOLOMICS

  • Isotope dilution GC/MS or LC/MS assays
  • Analysis of catecholamines
  • Kynurenine Pathway metabolite identification
  • Nitrates and Nitrites analysis
  • Glucose quantification
  • Hormone identification and quantification
  • Acetylcholine and other quaternary ammonium biological amines
  • Quantification of Vitamin D and metabolites
  • Assay for the quantification of dityrosine in urine and protein hydrolysates
  • Detection of lactones in microbial extracts
  • Analysis of coal tar samples for molecular weight distribution
  • Analysis of borate complex structure in plant extracts
  • Metabolite profiling by LC-MS or GC-MS
  • Untargeted metabolomics
  • Orbitrap LC-MS for accurate mass small molecule analysis
  • Triple quadrupole LC-MS for small molecule analysis
  • Ion trap LC-UV-MS for small molecule analysis
  • Small molecule quantitation by mass spectrometry
  • Drug quantitation in biological fluids by mass spectrometry
  • Targeted quantitation of specific molecules in biological fluids/tissues by mass spectrometry
  • Folate quantitation


LIPIDOMICS

  • Isotope dilution GC/MS or LC/MS quantification
  • Lipid analysis or profiling by high resolution LC-MS and MS/MS
  • Quantification of lipids by nanospray


IRMS

  • Kiel/IRMS analysis of carbonate
  • Elemental Analysis/IRMS


OTHER TECHNIQUES

  • Analysis of synthetic polymers and large synthetic organometallics
  • HPLC separations
  • UPLC separations
  • Surface analysis by DESI-Orbitrap
  • nano-DSC
  • nano-iTC
  • Synthetic Biology. ¬†Many of the analytical capabilities of the BMSF may be utilized in research area.