Biological Specimen Preparation

BSP Laboratory

Specimen quality is often a deciding factor in acquiring high quality microscopy images. BMIF established the Biological Specimen Preparation (BSP) Laboratory to aid researchers in preparing their samples for imaging on BMIF equipment. BSP offers a range of in-house sample preparation services, equipment access and training for researchers to prepare their own samples as required.

There are 3 main areas in BSP. For more information on any of the following services, please contact Dr. Renee Whan .

1. Routine and Advance Histology

We offer both routine and advanced histology specimen preparation. Our in-house sample preparation services include:

  • Sample processing (Paraffin embedding)
  • Slide sectioning (Paraffin and Frozen)
  • Histology staining
  • Immunohistochemical staining (single / multiple)
  • Special stains
  • Tissue Microarrays (TMA)
  • Multiplexed immunofluorescence staining

We also offer access to specimen preparation equipment access and training for researchers to prepare their own samples as required.

To access this service, please provide the completed and signed BSP Sample Preparation Request Form to Fei Shang or Merryn Brettle

Please click here for the current charges for this service.

2. Slide Scanning

We offer both bright-field (BF) and fluorescence (FL) slide scanning services. We also offer access and training to the slide scanning equipment for researchers to scan their own slides as required. BF slide scanning is performed on the Aperio XT scanner and the images are viewed in the ImageScope software. We can assist you with the setup of the slide analysis, if required.

In addition, we provide access to the Vectra Polaris system for both BF and FL slides and multiplexed immunofluorescent stained specimens (OpalTM Kit). The Vectra Polaris can process specimens prepared as whole tissue sections or tissue microarray (TMA) slides.

To access this service, please provide the completed and signed BSP Slide Scanning Request Form to Florence Tomasetig or Iveta Slapetova or Merryn Brettle

Please click here for the current charges for this service.

3. Specimen Processing for Electron Microscopy

We offer services in specimen processing for Electron Microscopy (EM) and Correlative Light and Electron Microscopy (CLEM), in collaboration with the Electron Microscopy Unit (EMU). These services include:

  • Cryo fixation (Leica ICE high pressure freezing)
  • Chemical specimen fixation (glutaraldehyde/paraformaldehyde)
  • EM sample processing (microwave assisted EM processing or AFS2-automated freeze substitution)
  • CLEM – Light microscopy

To access this service, please provide the completed and signed BSP CLEM Services Request Form to Iveta Slapetova.

Please click here for the current charges for this service.