Nikon Ti2 (Biosciences)

Nikon Ti2
The Nikon Eclipse Ti2-E is an inverted widefield system offering a large field of view, high-quality optics and an industry-leading focus stabilisation system (Nikon Perfect Focus System). he system software includes Nikon JOBS, allowing automation of experimental protocols, including on the go analysis and cell identification.  Additionally, a CoolLED pE-340Fura light source enables rapid ratiometric imaging of rapid calcium dynamics.
Microscope body: Inveted
Motorised Stage: Yes
Temp Control: Full Enclosure
CO2: Stage Insert
Software: NIS Elements

Common Applications

Live-cell imaging Imaging living cells allows observation of dynamic processes, greatly improving our understanding of how biological mechanisms work. To facilitate cell survival and to simulate biologically relevant conditions for cellular function, this system includes a temperature and atmosphere controlled stage insert. This wide-field fluorescence system allows gentle imaging of live cells with minimal phototoxic impact. Further, Nikon’s NIS Elements software allows for easy setup of long-term live-cell experiments for visualisation of cell migration and cell proliferation.
Calcium Imaging Using a rapidly changing light-source, such as a CoolLED pE340 Fura,  this system allows the switching of excitation in as little as 20 microseconds. This enables rapid ratiometric imaging to capture events on the order of microseconds.
Automated experiments This system is equipped with Nikon’s JOBS software, providing a platform for setting up automated experiments. Using a modular interface, JOBS allows users to pre-set imaging parameters and perform long-term experiments that run without further input.