Aperio XT Slide Scanner

The Aperio ScanScope XT scanner is a brightfield microscope that is used to digitalise traditional histochemicaly stained tissues mounted on a microscope slides. You can choose between 20X and 40X magnification and scan any required size of the tissue. The digital slides are viewed and analysed using Aperio software applications (ImageScope). The image format is .svs which is a form of a tiff file, that can be converted into JPEG2000 for faster processing and presentation. The Aperio XT scanner can autoload up to 120 glass slides (1x3") as well as manually scan your slides one by one if required.

Microscope body:
Upright
Motroised Stage:
Yes
Temp Control:
No
CO2:
No
Software:
ImageScope

 

Common Applications

Digitalization of microscope slides: Aperio XT is not using tiling but line scanning to digitalize the image a tissue histologically stained and mounted on a glass slide. The slide is accurately moved under a line-scan camera to acquire the image line by line. This process allow to reduce the number of image necessary to cove the area of interest (28000 image tiles are necessary to capture an area of 30 x 20 mm when only 60 images stripes are necessary; 0.25µm/pixel, x40 objective).

 

Objective Lenses

MAG N.A CORRECTIONS IMMERSION W.D MISC.
20x 0.75 Plan Apo Dry  

40x is obtained with a 2x magnification changer

20x: 0.50 µm/pixel 

40x: 0.25 µm/pixel  

 

Light Sources

Source Wavelenghts Nominal Power
FIBER-LITE DC950 ILLUMINATOR Visible light (quartz halogen) 150W

 

Detectors

Detector Sensor Type Pixel Format Pixel Size Description
8/10-bit monochrome TDI line-image capture camera Single line CCD     Scanning region: 23.4 x 55 mm

 

Mscellaneous

  • Image Compression: JPG2000
  • Image File Formats: TIFF, SVS, and JP2 (using JPEG2000 compression)
  • Manual Loader: 1 normal microscopy slide
  • Auto loader: up to 120 slides