FluoView FV1200

 
 

The FV1200 is a filter based upright confocal system. A workhorse of our confocal systems it is well equipped for standard fixed samples where high resolution and sensitivity are required for localization based studies. Additionally as one of the few upright confocal systems within the BMIF it provides a means for visualizing water submerged opaque samples.

Microscope body:
Upright
Motroised Stage:
Yes
Temp Control:
No
CO2:
No
Software:
FV10-ASW 4.1

 

Common Applications

Confocal: Confocal imaging is a fluorescence microscopy technique that optically sections the speciment preventing out of focus light from reaching the detector. This yields clear high contrast images, and together with the ability to acquire images sequentially at multiple positions the entire sample can be reconstructed in 3D
Colocalisation: Light microscopy lends itself very well to labelling multiple structures within a sample due to the ability to separate these spatially overlapping signals by the wavelength of light they emit. Colocalisation is the study of how the distribution of one probe relates to that of another within the same sample.

 

Objective Lenses

MAG N.A CORRECTIONS IMMERSION W.D MISC.
4x 0.16 UPlanSApo AIR 13mm  
10x 0.4 UPlanSApo AIR 3.1mm  
20x 0.75 UPlanSApo AIR 0.6mm  
40x 1.30 UPlanFL N OIL 0.2mm  
60x 1.35 UPlanSApo OIL 0.15mm  
100x 1.40 UPlanSApo OIL 0.13mm  

 

Light Sources

Confocal
  • Solid State lasers: 405nm, 473nm, 559nm, 635nm
 
Widefield (eyepiece only)

Filters

Confocal
Each reflective PMT has a selection of dedicated filters associated with it
 
PMT 1 PMT 2 PMT 3

BP 465 - 495

BP 490 - 540

BP 430 -455

BP 490 - 540

BP 575-620

BP 535-565

BP 490-540

BP 575 - 675

BP 655 - 755

 

Widefield (eyepiece only)
DAPI. FITC, TRITC

Detectors