Nikon A1 Intravital

 
 

The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability. 

 

Microscope body:
Inverted
Motroised Stage:
Yes
Temp Control:
Full Enclosure
CO2:
Stage Insert
Software:
NIS Elements

Common Applications

 
Intravital: Intravital Imaging is a term used to describe the imaging of whole tissues and organs. It often requires specific adaptations to an instrument such as hydraulic stages, multiphoton lasers and equipment specific to sample preservation
Frap:

Fluorescence Recovery After Photobleaching is used to gain insight into the dynamics or both diffusion and binding rates within a sample. Photobleaching is an irreversible process by which a fluorophore losses the ability to absorb and emit light. By deliberately inducing photobleaching within a restricted region of the sample the observed recovery within this region over time is affected by the rate at which unbleached molecules entre this region and bleached molecules are removed.

Colocalisation:

Light microscopy lends itself very well to labelling multiple structures within a sample due to the ability to separate these spatially overlapping signals by the wavelength of light they emit. Colocalisation is the study of how the distribution of one probe relates to that of another within the same sample. 

 



Objective Lenses

 
MAG N.A CORRECTIONS IMMERSION W.D
4x 0.2 CFI Plan Apochromat AIR 20 mm
10x 0.45 CFI Plan Apochromat AIR 4 mm
20x 0.75 CFI Plan Apochromat AIR 1 mm
40x 1.25 CFI Apochromat WATER 0.18 mm
60x 1.2 CFI Plan Apochromat WATER 0.31 - 0.28
60x 1.4 CFI Plan Apochromat OIL 0.13 mm

 

 


Light Sources

Confocal
Lasers: 405, 488, 561, 640
 
Widefield (Eyepiece Only)
Mercury Light Source: Nikon Intensilight C-HGFIE
 
 


Filters

 
ch1 PMT Ch2 GaAsP Ch3 GaAsP Ch3 PMT
BP 425 - 475 BP 500 - 550 BP 570 - 620 BP 662-737
BP 464 - 500 BP 525 - 555 BP 552 - 618  
  BP 500 - 530    

 

Dichroics

405/488

405/488/561

405/488/531/640

405/488/543

405/488/543/640

400-457/514

457
BS 20/80
 
 


Detectors

 
1x Transmitted PMT
2x Fluorescent/Reflective PMT
2x Fluorescent/Reflective GaAsP
 
 


Miscellaneous

 


Pefect Focus System To Be Completed
OKO labs Cage Incubator To Be Completed
OKO labs objective heater To Be Completed