Zeiss Elyra 7

The Zeiss Elyra 7 offers several super-resolution modalities, allowing detailed visualisation of samples beyond the diffraction limit of conventional light microscopy. With 2 fast CMOS cameras, this system allows users to rapidly image multiple colours. The Elyra 7 features Lattice SIM (Structured Illumination Microscopy), allowing fast and gentle super-resolution imaging (~120 nm in xy and ~300 nm in z) in 3 dimensions. The speed of this imaging modality is well suited to capturing dynamic biological processes in live samples. In addition, the Elyra 7 features TIRF imaging for improved signal to noise, and single-molecule localisation imaging for STORM, PALM and PAINT experiments (~20-30 nm xy and ~50-80 nm in z) on fixed samples.
Microscope body: Inverted
Motorised Stage: Yes
Temp Control: Microscope enclosure and stage insert
CO2: Stage insert
Software: Zen 3.0 SR (Black Edition)

Common Applications

Lattice SIM Lattice Structured Illumination Microscopy involves illuminating a fluorescent sample with a ‘lattice’ illumination pattern. A series of images are acquired with the illumination pattern in different orientations, giving spatial information that normally would be lost. The various illumination pattern orientations require 3 rotations and 5 phase shifts for a total of 15 images in order to reconstruct one super-resolution image. Additionally Elyra 7 features ‘Apotome mode’, a faster mode that forgoes the illumination pattern rotations, instead using 5 phase shifts to quickly acquire 2-dimensional image and facilitate optical sectioning. The high speed of the CMOS cameras used in this setup makes this technique ideal for imaging live cells.
STORM Stochastic Optical Reconstruction Microscopy is a super-resolution microscopy technique utilising a TIRF architecture to illuminate the first 100-200 nm of a sample and image single fluorescent molecules. This is achieved by converting fluorophores from a dark to active state using an appropriate buffer solution, before bleaching a short time later. At any time only a very small portion of the fluorophores are active and with each dark-active-bleach cycle more and more of the underlying sample is revealed at up to 10x the resolution of conventional techniques.
PALM Photo-Activated Localisation Microscopy is a super-resolution microscopy technique where the principle of detection and analysis is the same as STORM, but in this case it is not anti-body based labelling and does not require special buffers, but rather requires use of photo-activatable genetically encoded proteins.
DNA-PAINT Similar in principle to STORM and PALM, DNA-based Point Accumulation for Imaging in Nanoscale Topography relies on labels cycling between a fluorescent and dark state to resolve individual molecules. Unlike PALM and STORM, DNA-PAINT achieves cycling through molecular means. Samples are labelled with antibodies conjugated to a single strand of DNA and are treated with a solution of complementary single-stranded DNA conjugated to fluorophores. The continuous binding and unbinding of fluorophore-bound DNA produces a similar cycling when compared to STORM.

Objective Lenses

10x 0.3 EC Plan-Neofluar Air 5.2 mm
63x 1.2 C-Apochromat Water 0.28 mm
40x 1.4 Plan Apochromat Oil 0.13mm
63x 1.4 Plan Apochromat Oil 0.19 mm
63x 1.46 Alpha Plan-Apochromat Oil 0.11 mm


FSet 77 HE 

Transmission Light 

LBF 405/488/561/642 

BP 420-480 + BP 495-550 + LP 655 

BP 495-550 + BP 570-620 

BP 420-480 + LP 655 



LP 560 

BP 490-560 + LP 640 


2x PCO-edge sCMOS cameras

  • 82% quantum efficiency
  • 1280 x 1280 pixels
  • 6.5 μm x 6.5μm pixel size
  • Dynamic range 16 bit