Zeiss Lightsheet Z.1

 

Lighsheet microscopy is a fluorescence-based imaging technique that is growing in popularity. It outperforms other techniques in its ability to rapidly capture large optically sectioned three dimensional datasets, whilst keeping photo-toxicity and bleaching to a minimum. It therefore lends itself well to imaging samples larger than those typically attempted by conventional fluorescence microscopy such as tissues, whole organs and even embryo development. 

Microscope body:
Custom
Motroised Stage:
Yes
Temp Control:
Heated Chamber
CO2:
Stage Insert
Software:
Zen

Common Applications

 
Lightsheet microscopy Lighsheet microscopy creates and positions a sheet of excitation light across the focal plane of a collection objective. In doing so it restricts excitation events in a particular plane of the sample resulting in an optically sectioned image drastically reducing light emanating from outside of the focal plane. Moving the sample through this plane allows rappid collection of sectioned 3D data.
Tissue Clearing: Tissue clearing is a preparation technique with the aim of reducing the inherinatly scattering nature of tissue. There are several means by which this is achieved but all enable clearer and deeper penetration into the sample using light microscopy.
   


Objective Lenses

 
MAG N.A CORRECTIONS IMMERSION MISC.
5x 0.16 Plan Neofluar Air  

20x

1 Plan Apochromat Water nd = 1.32-1.4
20x Clearning 1 Plan Neofluar Clearing nd = 1.42-1.45
40x 1 Plan Apochromat water  

 

 


Light Sources

Laser lines: 405 nm, 445 nm, 488 nm, 515 nm, 561 nm, 638 nm
 
 


Filters

 
The system can hold one of two filter wheels positined just before the two cameras. Note these filters are not to separate the excitation and emission lightpath (as these are already separate), these filters are designed to split the emitted light to separate cameras for simultaneous imaging.
 
Camera Filter Wheel 1
NAME Camera 1 DICRHOIC Camera 2
Universal Blue-Green

SP 490

BS 490 LP 505
CFP-mCherry BP 460-500 BS 510 LP 585
GFP-RFP

BP 505-545

BS 560

BP 575-615

YFP-RFP BP 525-545 BS 560 BP 575-615
CY3-DRAQ5 BP 575-615 BS 640 LP 600
 
 
Camera Filter Wheel 2
NAME CAMERA 1 DICRHOIC CAMERA 2
DAPI-CY3

BP 420-470

BS 510 BP 575-615
CFP-YFP BP 460-500 BS 510 BP 525-565
GFP-RFP

BP 505-545

BS 560

BP 575-615

GFP-DRAQ5 BP 505-545 BS 560 LP 660
GFP-mCherry BP 505-530 BS 560 LP 585
 


Detectors

 

This instrument is equipped with 2 PCO Edge 5.5 cameras. They are configured to allow simultaneous imaging of two channels