Zeiss LSM 800

zeiss 800

The Zeiss LSM 800 is an inverted Zeiss Axio Observer Z1 laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector, 2 multi-alkali PMTs and DIC optics for trans-illumination microscopy with each objective. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (120 nm in x / y and 350 nm in z) and 4-8 x better signal-to-noise ratio, with fast linear scanning. Airyscan works with thicker samples such as fixed tissue sections or whole animal mounts that need a higher penetration depth.

LSM 800 provides sensitive image acquisition leading to better flexibility in live cell imaging and high-end image quality, well suited for various research areas. The high sensitivity of the detectors allows detection of weak fluorescent signals while employing low levels of laser illumination and rapid frame rates. The system is capable of spectral imaging and unmixing, and remove autofluorescence background from low signals in fixed brain tissue. The acquisition software can acquire and stitch tiled images of samples too large to fit within the field of view. It can easily be programmed to capture images of multiple regions on a slide or multi-well plate.

Microscope body: Inverted
Motroised Stage: Yes
Temp Control: Stage Insert
CO2: Stage Insert
Software: Zen Blue 2.5

 

 

Common Applications

Confocal: Confocal imaging is a fluorescence microscopy technique that optically sections the specimen, preventing ‘out of focus’ light from reaching the detector. This yields clear and high contrast images, and together with the ability to acquire images sequentially at multiple positions, the entire sample can be reconstructed in 3D.
Spectral Unmixing:

Conventionally, imaging multiple fluorophores of the same sample requires careful selection to ensure crosstalk and/or bleedthrough do not lead to confusion between the signals. This can greatly restrict the number of probes available to you. Spectral unmixing is a technique that uses information about the spectral profile of each fluorophore to mathematically separate the overlapping signals.

Colocalisation:

Light microscopy lends itself very well to labelling multiple structures within a sample due to the ability to separate these spatially overlapping signals by the wavelength of light they emit. Colocalisation is the study of how the distribution of one probe relates to another within the same sample.

Enhanced resolution (Airyscan):

The Airyscan detector consists of a hexagonal array of 32 GaAsP/PMT detector elements that have the light collection efficiency of a 1.25 AU pinhole (up to 50% more light collected than with a 1 AU pinhole). Each detector element functions similarly to a single, small (0.2 AU) pinhole, which enables the generation of higher (1.7 x) resolution images with improved signal-to-noise ratio than a standard confocal. The Fast mode can be used to increase acquisition speed to up to 13 fps by elongating the excitation beam to acquire 4 lines of image at once, without sacrificing resolution or signal-to-noise.

Short term live cell culture: Live cells can be imaged in an automated manner using the Experiment Designer software tool, combined with three parallel spectral channels. The motility of subcellular structures can be studied using Airyscan GaAsP detector to image with time lapse imaging in 2D or 3D at 1.6 frames per second

Objective Lenses

MAG

N.A.

CORRECTIONS

IMMERSION

 

W.D.

10x

0.45

Plan-Apochromat

Air

 

2mm

20x

0.8

Plan-Apochromat

Air

 

0.55mm

40x

1.3

Plan-Apochromat

Oil

 

0.21mm

63x

1.4

Plan-Apochromat

Oil

 

0.19mm

Light Sources

Source

Wavelengths

Nominal Power

405 Laser

405 nm

5 mW

488 Laser

488 nm

10 mW

561 Laser

561 nm

10 mW

640 Laser

640 nm

5 mW

Filters

Confocal Internal Detectors

Tunable wavelength selection for all 3 fluorescence/reflective PMTs between 400 - 750nm. Clean up emission filters for PMT channels are also available.

 

Widefield (Eyepiece Only)

NAME

EXCITATION

DICHROIC

EMISSION

Filter Set 49 (DAPI)

365

LP 395

BP 445 / 50

Filter Set 10 (Alexa 488)

BP 450 - 490

LP 510

BP 515 - 565

Filter Set 20 (Rhodamine)

BP 546 / 12

LP 560

BP 575 - 640

Detectors

2 multi-alkali (MA) PMT (typical QE 25 %)

1 Airyscan (32 channels GaAsP) optimised for 63× objective

1 Transmitted light detector (T-PMT)