GE and BioRad mini and large format SDS-PAGE systems

2DE

Proteomic work can involve the use of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE or 2-DE). In 2-DE, "first dimension" electrophoresis in a polyacrylamide gel with a pH gradient and high urea concentration is followed by a "second dimension" separation in an SDS-PAGE gel.

SDS-PAGE can also be used independently. Proteins and peptides are separated within the gel according to the % acrylamide and crosslinker, given as %T and %C. Typical separations are homogenous: 7% for high mass protein separation and up to 15%T for low mass proteins; 2.7%C; gradient gels can also be run (4-20%T).

Peptides can also be separated using a compatible buffer system. A typical buffer system is Tris/glycine/SDS, however other combinations may be more suitable to your sample. Native and denaturing gels can be prepared. Choice will depend on sample requirements.

Contact People

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Dr Valerie Wasinger

Position: Senior Research Scientist
Category: Academic Staff

+61 (2) 9385 1678
+61 (2) 8214 6831