GE Healthcare iTC200 Isothermal Calorimeter

Investigating molecular interactions, reaction kinetics and thermodynamics by Isothermal Calorimeter (iTC). Production or absorption of heat is a universal event consequent to the binding of two molecules or a chemical reaction. iTC is the only equipment that can directly measure the energetics of biochemical processes involving interaction between two molecules such as a receptor and a ligand. A receptor can be any biopolymer such as a protein/enzyme, nucleic acid, carbohydrate, lipid etc, whereas a ligand can be either a large or small molecule (such as a metal, drug, inhibitor, activator, toxin, substrate, protein/peptide, sugar etc). In addition to determining the affinity and stoichiometry of molecular binding, iTC has the ability to precisely measure the free energy, enthalpy, entropy and heat-capacity changes associated with binding and reaction. The current iTC200 requires only 0.2 ml of sample and 0.04 ml of titrant, is highly sensitive (can detect a heat signal of 0.2 µcal/sec) and takes less than an hour. iTC200 can also be upgraded to provide sample throughput of up to 50 samples per day with a capacity to process 384 samples unattended.

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