Zeiss LSM 900 Cryo

Zeiss LSM 900 Cryo

This LSM 900 is an upright high-end confocal microscope. Equipped with GaAsP detectors and an Airyscan 2 detector it delivers speed and sensitivity without compromising signal to noise. A new multiplex mode for Airyscan 2 gives you the ability to choose between an increase in resolution by 1.7x, improve your signal to noise, or increase you acquisition speed. Additionally, it has a cryo stage facilitating specimen preparation for electron microscopy, allowing correlative microscopy between light microscopy on living specimens, and the high resolution of electron microscopy.

Microscope body:	Upright
Motorised Stage:	Yes
Temp Control:	Cryo Stage for Freezing
CO₂:	No
Software:	Zen Blue 3.1

Confocal	Confocal imaging is a fluorescence microscopy technique that optically sections the specimen, preventing ‘out of focus’ light from reaching the detector. This yields clear and high contrast images, and together with the ability to acquire images sequentially at multiple positions, the entire sample can be reconstructed in 3D.
Colocalisation	Light microscopy lends itself very well to labelling multiple structures within a sample due to the ability to separate these spatially overlapping signals by the wavelength of light they emit. Colocalisation is the study of how the distribution of one probe relates to another within the same sample.
Enhanced resolution (Airyscan):	The Airyscan detector consists of a hexagonal array of 32 GaAsP/PMT detector elements that have the light collection efficiency of a 1.25 AU pinhole (up to 50% more light collected than with a 1 AU pinhole). Each detector element functions similarly to a single, small (0.2 AU) pinhole, which enables the generation of higher (1.7 x) resolution images with improved signal-to-noise ratio than a standard confocal. The Fast mode can be used to increase acquisition speed to up to 13 fps by elongating the excitation beam to acquire 4 lines of image at once, without sacrificing resolution or signal-to-noise.

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