Thermo LTQ Orbitrap Velos ETD

Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Orbitrap Velos ETD is a hybrid instrument that incorporates both Orbitrap and linear ion trap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments. Detailed insights into analyte molecule structural characteristics can be obtained via tandem and multiple stage mass spectrometry (MS/MS and MSn) experiments, in which various different methods of ion activation may be utilised. Analyses are frequently performed upon complex peptide mixtures generated via the enzymatic or chemical hydrolysis of proteins, allowing confident protein identifications to be readily made at low fmol levels. Quantitative analyses are possible via, for example, the isobaric labelling of peptides. Detailed characterisations of post-translational modifications (PTMs) are capable of being achieved via the various modes of ion activation available during MS/MS; in this regard, subjecting analyte peptides to Electron Transfer Dissociation (ETD) can be particularly useful: labile PTMs are left intact whilst structural information is obtained via peptide bond fragmentation.

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