Applied Photophysics Chirascan Plus CD

Applied Photophysics Chirascan Plus CD

Circular Dichroism: Applied Photophysics Chirascan Plus CD spectrometer for analysing secondary structure or conformation of macromolecules or gels.

The Chirascan CD is used for:

  • Analysis of secondary structure or conformation of macromolecules or supramolecular aggregates in solution.
  • measuring changes in secondary structure due to binding events between molecules.
  • measuring changes in secondary structure due to temperature changes (eg. oligonucleotide melting) or chemical denaturation.

Circular dichroism (CD) is the difference in the absorbance of left-handed circularly polarised light (L-CPL) and right-handed circularly polarised light (R-CPL) and occurs when a molecule contains one or more chiral chromophores (light-absorbing groups). A primary use is in analysing the secondary structure or conformation of macromolecules, particularly proteins, and because secondary structure is sensitive to its environment, e.g. temperature or pH, circular dichroism can be used to observe how secondary structure changes with environmental conditions or on interaction with other molecules.

Cuvette holder options: 1 cm, 1 mm, 0.5 mm, 0.2 mm and 0.1 mm.

Variable temperature accessory for 1 cuvette or 4 cuvettes.

For further information on instrument training, please contact Dr Brit David.