The Faxitron MX-20 is a self contained bench-top x-ray cabinet which produces digital 2D radiographs in either png or jpg file formats. It has 5 magnification levels and laser guide for sample alignment.
The Zeiss LSM 880 is an inverted laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector and 2 multialkali PMTs, which can be used as single point detectors or combined for 34 channel spectral imaging. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (140nm axially, 400 nm laterally) and 4-8 x greater signal-to-noise ratio, at the highest speed of any linear scanning confocal system. BiG.2 Type A (2x GaAsP detectors) non-descanned detectors for the multi-photon laser can be used for imaging thick specimens, with deeper penetration and decreased phototoxicity compared to single photon lasers. The Picoquant system utilises the multi-photon laser to perform Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Forster Resonance Energy Transfer (FRET). The Zeiss 880 is also equipped with polarisers for anisotropy measurements.
The Inveon μCT uses multiple axial x-rays of the animal to generate cross-sectional information or 3-dimensional reconstructions of the animal or parts of the animal.The μPET uses an LSO detector for fast scintillation decay time, high light output and effective atomic number.
The 3i VIVO microscope is ideally suited for intravital imaging. Its large stable stage can easily accommodate sizable tissue samples along with clamps and manipulators to aid stabilization. Equipped with a spinning disk unit for high speed optical sectioning, paired with an image intensifier, this system can achieve very high frame rates making the most of every photon. Additionally, a pulsed 532nm ablation laser is available for targeted tissue damage.
The BD LSRFortessa™ SORP analyzing flow cytometer is a high-end, user-friendly flow cytometer with 5 excitation lasers and 24 detection parameters (matching our BD Influx’s configuration). Optimised for both biomedical and environmental research, this cytometer offers an optional High Throughput Sampling system, and small particle detection.
This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet (HiLo: https://www.nature.com/articles/nmeth1171) modalities in one instrument. The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample. The system is also supplemented with NIDAQ board and control with external trigger, which combined with the versatile illumination sequence option makes this ideal microscope for complex experiments involving the correct timing of imaging following an external stimulus of cells or animal. Definite focus is perfectly controlled using Nikon PFS system making it ideal for long term live cell or animal imaging.
The BD FACSAria™ IIIu cell sorter is equipped with five excitation lasers (violet 405nm, yellow/green 561nm, red 642nm and blue 488nm OR true-blue 445nm) and can detect up to 17 parameters (15 fluorescence channels plus forward scatter and side scatter).
EasyGel is a system designed for speeding the process of producing tissue-gel-hybridization samples. The SmartClear II system is a commercial instrument that allow fast, easy and reliable tissue clearing of tissue-gel-hybridization samples. The system uses stochastic electrotransport technology to remove lipids from tissue-gel-hybridization samples, thereby producing optically transparent samples. Samples that have been optically cleared with the SmartClear II system can be imaged by a number of light microscopy techniques such as confocal and lightsheet.
Leica RM2135 rotary microtome is used to cut very thin cross-sections of specimens (usually 4 µm) for routine and research histology studies in the fields of biology and medicine. This instrument is designed for cutting soft and harder paraffin embedded specimens, as long as they are suitable for being cut manually. The sectioning thickness setting range can be from 1 µm to 60 µm. High quality paraffin sections could be used for varying staining, such as Haematoxylin and Eosin stain (H&E), immunohistochemistry (IHC), Immunofluorescent staining (IF) and In situ hybridization (ISH).