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Leica TCS SP5 MP STED
Nikon TIRF/HiLo
This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet (HiLo: https://www.nature.com/articles/nmeth1171) modalities in one instrument. The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample. The system is also supplemented with NIDAQ board and control with external trigger, which combined with the versatile illumination sequence option makes this ideal microscope for complex experiments involving the correct timing of imaging following an external stimulus of cells or animal. Definite focus is perfectly controlled using Nikon PFS system making it ideal for long term live cell or animal imaging.
Nikon Eclipse Tie
For UNSW Staff
Grant development and review
Dr Sandra Fok
Position: Research Associate (Biomedical Imaging)
Facility:
Biomedical Imaging Facility
Category: Academic Staff
Dr Richard Francis
Position: Research Associate (Intravitals)
Facility:
Biomedical Imaging Facility
Dr Elvis Pandzic
Position: Research Scientist (Advanced Fluorescence Microscopy)
Facility:
Biomedical Imaging Facility
Dr Rhiannon Kuchel (Maternity Leave)
Position: Research Associate
Facility:
Electron Microscope Unit
Category: Academic Staff
Education
PhD Marine Science Cell Biology/Immunology, Macquarie University (2012)
Role and Interests
Rhiannon Kuchel is an electron microscopist at the University of New South Wales, (Sydney). With a PhD from Macquarie University her thesis focused on the effects of environmental stress on the immune system of temperate pearl oysters. Rhiannon’s role at the EMU is to conduct collaborative research as well as teach postgraduate students from a variety of disciplines (biology, chemistry, materials science/engineering), focusing on the use of transmission (both standard imaging and cryo) and scanning electron microscopy.
Publications
Mrs Sigrid Fraser
Position: Technical Officer
Category: General/Technical Staff
Qualifications
Diploma from University of Kiel,Pharmaceutical Biology Dept., Germany
ech. Staff at University of Goettingen, Plant Physiology Dept., Germany
Sydney Eye Hospital, Pathology Dept.
Role and Interests
Training and support for SEM 3400, TEM 1400, Cryo-and Room Temperature Ultramicrotomy, Specimen Preparation
MWAC Staff Workshops
The aim of these workshops is to promote cross-facility awareness of the growing capabilities of the Mark Wainwright Analytical Centre. Each session will demonstrate particular capabilities within a facility to promote discussion and interaction across MWAC. They will also provide staff with an opportunity to see how other facilities function.
Empyrean 3 (PANalytical) XRD with Ag source and PSD
The Empyrean 3 is fitted with a silver source and a position-sensitive detector to instantly capture a wide 2-theta angular range during an in situ XRD experiment without the need to scan the detector. The instrument can run capillary samples and the high energy silver source also enables the instrument to perform the pair distribution function (PDF) experiment that describes the distribution of distances between pairs of atoms within a material.
Smartlab (Rigaku) 9kW rotating anode thin-film XRD
High intensity (9kW) rotating copper anode X-ray source for rapid analysis of thin films.
SC-XRD Kappa Apex (Bruker) Single Crystal Diffractometer
The Bruker Kappa Apex is fitted with an Incoatec molybdenum X-ray microfocus source to determine the molecular and crystal structure of materials from a single crystal. The instruments is usually operated on a service-basis by an experienced crystallogrpahy, although user training is encouraged.
D8 (Bruker) Thin-Film XRD with 5kW Rotating Anode
The D8 is a flexible instrument with a rotating copper anode X-ray source (5-6 kW) that enables a higher X-ray flux at your sample than is possible with the sealed X-ray sources on the other instruments. It uses a centric Eulerian cradle that integrates Chi and Phi rotations, and X-Y-Z translations. The instrument can be set up with a 1-dimensional compound silicon strip detector (the "Lynx-Eye") for ultra fast X-ray diffraction measurements, as well as a large area detector (the "VÅNTEC-500" with 13.5 cm diameter) for 2D XRD experiments including RSM and diffraction mapping.
MRD (PANalytical) Xpert Materials Research diffractometer
The MRD is a highly flexible X-ray diffraction system, equipped with X-ray mirror, (220) Ge monochromator, X-ray lens, mono-capillary, triple-axis goniometer, and is used for analysis of thin films, nanostructure, semiconductor materials, stresses and textures. It is equipped with a few active components, such as an X-ray mirror, Ge 220 monochromator, X-ray lens and mono-capillary for incident beam conditioning, and parallel plate collimator or triple-axis for diffracted beam conditioning. Both proportional and area detectors are available.
Empyrean 1 (PANalytical) Thin-Film XRD
The Empyrean Thin-Film XRD installed in 2013, provides thin-film analysis or BB geometry with variable temperature stages and reaction cell for in situ studies.
MPD (PANalytical) Xpert Multipurpose X-ray Diffraction System
The MPD is a Bragg-Brentano geometry X-ray diffractometer suitable for routine measurement of powder samples, either phase identification or quantitative analysis. The instrument is equipped with the following components:
Empyrean 2 (PANalytical) X-ray Diffraction System (Cobalt)
The Empyrean 2 is a Bragg-Brentano geometry X-ray diffractometer that was installed in 2014 and is equipped with the following components:
- Cobalt anode X-ray tube to minimise fluorescence from iron-containing samples that occurs when using the standard copper X-ray anode
- High resolution optic for cobalt X-rays to minimise scatter at low angles
- Sample changer for large batches