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Thermo Scientific CryoStar NX70

Specimen Preparation

Thermo Scientific CryoStar NX70 is a high-performance cryostat designed to prepare tissue

sections of frozen samples in medical and research laboratories. Specimen temperature control range is from -50oC to +10oC and blade temperature control range is from -35oC to -5oC.  Section thickness can be ranged from 0.5 µm to 500 µm. Fresh or fixed frozen specimens, embedded in OCT, are suitable for cryostat sectioning.  Frozen sections could be used for varying staining, such as histology stains, Immunohistochemistry (IFC) and Immunofluorescence (IF) stains.

TILL Photonics iMic TIRF System

Fluorescence Widefield TIRF FLIM

This microscope has a unique design, allowing for a great deal of customization of the sample space and experimental design. The system is designed for up to four-color total internal fluorescence (TIRF), photoactivation (PA), fluorescence resonance energy transfer (FRET), widefield correlation spectroscopy (WCS), spatio-temporal image correlation spectroscopy (STICS), and fluorescence recovery after photobleaching (FRAP) experiments, all at high frame rates. Single-molecule measurements can be enhanced with a pair of single-photon avalanche photodiodes (APDs) and a pulse correlator for fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) experiments. An environmental chamber allows for these techniques to be extended to live cell samples.

Vectra Polaris (UNSW & Ramaciotti)

Slide Scanning Multi-plexing

The Vectra® Polaris™ Automated Quantitative Pathology Imaging System is a new class of tissue imager which provides unparalleled speed, performance, and versatility for visualizing, analyzing, quantifying, and phenotyping immune cells in situ in Formalin Fixed Paraffin Embedded (FFPE) tissue sections and TMAs to advance disease research.

VisualSonics Vevo 3100 Ultrasound

The VisualSonics Vevo3100 uses the new solid-state transducers which offer higher resolution at multiple focal depths. The system offers superior resolution down to 30μm, a wide range of frequencies (13-56 MHz) and a large field of view. Importantly, the Vevo3100 offers a comprehensive imaging and processing packing.

Zeiss Cell Discoverer 7 LSM 900 (Ramaciotti)

Fluorescence Widefield Confocal

The Zeiss Celldiscoverer 7 is a boxed imaging system suited for performing automated experiments. This inverted widefield system features automatic focusing, focus stabilisation, time-course and position setup to allow researchers to insert a sample and set up long-term experiments in minutes

The Celldiscoverer 7 has a fully temperature-controlled sample chamber with a CO2 pump, this chamber is highly stable and allows for live cells to stay healthier for longer. By utilising LEDs as a light-source for widefield imaging, live-cells can be imaged gently and with low phototoxicity, making the Celldiscoverer 7 ideal for long-term live experiments. Additionally, an infrared LED is used for transmitted light brightfield and phase contrast imaging through all available objectives and through a variety of sample holders.

Zeiss Celldiscoverer 7

Fluorescence Widefeild

The Zeiss Celldiscoverer 7 is a boxed imaging system suited for performing automated experiments. This inverted widefield system features automatic focusing, focus stabilisation, time-course and position setup to allow researchers insert a sample and set up long-term experiments in minutes. The Celldiscoverer 7 has a fully temperature-controlled sample chamber with a CO2 pump, this chamber is highly stable and allows for live cells to stay healthier for longer. By utilising LEDs as a light-source, live-cells can be imaged gently and with low phototoxicity, making the Celldiscoverer 7 ideal for long-term live experiments. Additionally, an infrared LED is used for transmitted light brightfield and phase contrast imaging through all available objectives and through a variety of sample holders.

Zeiss Elyra 7

TIRF Super-resolution SIM Funded by Ramaciotti Foundation

The Zeiss Elyra 7 offers several super-resolution modalities, allowing detailed visualisation of samples beyond the diffraction limit of conventional light microscopy. With 2 fast CMOS cameras, this system allows users to rapidly image multiple colours. The Elyra 7 features Lattice SIM (Structured Illumination Microscopy), allowing fast and gentle super-resolution imaging (~120 nm in xy and ~300 nm in z) in 3 dimensions. The speed of this imaging modality is well suited to capturing dynamic biological processes in live samples. In addition, the Elyra 7 features TIRF imaging for improved signal to noise, and single-molecule localisation imaging for STORM, PALM and PAINT experiments (~20-30 nm xy and ~50-80 nm in z) on fixed samples.

Zeiss Elyra PALM/SIMS Superresolution

TIRF Super-resolution SIM

The Zeiss Elyra Superresolution Microscope is a developmental superresolution microscope with capabilities to push imaging past the classic diffraction limit. Typically visible light microscopes cannot achieve spatial resolution of greater than 200-250 nanometers. This system has two different imaging modalities to push this limit as low as 20 nanometers.