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Nikon Biostation

Nikon Biostation

Nikon Biostation is an inverted life cell imaging microscope enclosed in an incubator. The stage and objective are fully enclosed in temperature modulated box resulting in no focus drift during imaging. The cells are kept at 37C and supplemented with 5% CO2 throughout the entire experiment. The imaging chamber can hold 35mm glass or plastic bottom dishes of 0.17um thickness. The Biostation is an ideal microscope for imaging cell migrations, wound healing, cell division and cell growth and adherence.

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Nikon Eclipse Tie

Nikon Eclipse Tie

The Nikon Eclipse TiE is ideally suited for live cell imaging studies. With one of the best focus stabilisation devices on the market the Perfect Focus System (PFS) ensures that the focus you set at the start of the experiment will be maintained throughout its entirety. They system is fitted with several phase contrast objects making it suitable for imaging cells through a range of materials including plastic, as well as 3 high NA DIC objects for achieving astounding detail if imaging through glass.

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Nikon TIRF/HiLo

This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet (HiLo: https://www.nature.com/articles/nmeth1171) modalities in one instrument. The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample. The system is also supplemented with NIDAQ board and control with external trigger, which combined with the versatile illumination sequence option makes this ideal microscope for complex experiments involving the correct timing of imaging following an external stimulus of cells or animal. Definite focus is perfectly controlled using Nikon PFS system making it ideal for long term live cell or animal imaging. 

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NuSep ProteomSep/MF10 Electrophoretic Partitioning Systems

This in-solution electrophoretic device is for the partitioning of μl volumes of μg-mg amounts of proteins and or peptides based on surface charge, pH, size in denaturing or native formats. It is typically used to partition contaminant proteins away from lower abundance proteins (eg. Albumin or Ig from other plasma proteins), desalting of biological samples (eg. urine, plasma, tears), enrichment of poorly concentrated and low abundance samples for further analysis. Partitioning down to 1kDa is achievable and is therefore ideal for peptide and small protein work.

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Off-line Separation

1 dimensional & 2 dimensional gel eletrophoresis (SDS PAGE & 2DE) and in-solution electrophoretic partitioning are important off-line separation methods for large biomolecules such as proteins and nucleic acids. The BMSF has a comprehensive range of separation devices and mass spectrometers to address most analytical problems suited to mass spectrometry.

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Olympus Delta Premium Portable XRF Spectrometer

The Olympus Delta Premium PXRF Spectrometer was purchased in 2014. This is normally a service only instrument, but user training is possible if you contact the lab. The instrument comes with two factory calibrations (Geochem and Soil), as well as calibrations for coal, and can be calibrated to suit a specific sample type.

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PANalytical AXIOS Advanced Simultaneous WDXRF Spectrometer

The PANalytical AXIOS-Advanced WDXRF spectrometer was commissioned in 2010 and is fitted with an SST-MAX X-ray sourcewith a 4kW output and can be operated at 160mA. The resulting high X-ray flux enables high sensitivity and low detection limits for light elements. The AXIOS can measure elements over wide dynamic concentration ranges and include all the elements between Be and U in the periodic table.

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PANalytical PW2400 Sequential WDXRF Spectrometer

The PW2400 was purchased in 1996 and has a 102 position sample changer for unattended operation of large batches of samples. The instrument runs SuperQ and Uniquant software and is designed for qualitative, semi-quantitative, and quantitative analysis of a large number of elements, from beryllium to uranium. Trace element levels from 10 ppm to major elements up to 100%.

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PerkinElmer FTIR Spectrometer/Microscope

PerkinElmer FTIR Spectrometer/Microscope

The Spotlight is a Fourier transform infrared (FTIR) spectrometer fitted with with a microscope and attenuated total reflectance (ATR) accessories.

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PerkinElmer Raman Microscope (785 nm)

PerkinElmer Raman Microscope (785 nm)

Raman microscope to enable micron-scale chemical and structural mapping of samples as well as standard point spectra of solids, powders and liquids.

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PerkinElmer UV-Visible Spectrometer

PerkinElmer UV-Visible Spectrometer

The Lambda is a double-beam UV-Visible spectrometer that measures light absorption from the visible to the ultraviolet region (1100 nm to 200 nm, 1.1 eV to 6.2 eV). A sample is usually made up to a known concentration in a non-absorbing solvent (such as water) and loaded into a quartz cuvette (available from the lab). Liquids, solutions and transparent solids can be measured. Note that we are not able to measure reflectance on this instrument.

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Phillips CM200

Phillips CM200

The Philips CM200 field emission transmission electron microscope allows very high resolution images to be obtained from thin (electron transparent) materials. In a field emission gun a very strong electric field is used to extract electrons from a metal filament. This results in an electron beam which is very bright. Ultimately, this microscope allows individual atoms to be imaged. The CM200 TEM allows structural, crystallographic and elemental studies of materials.

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Picoquant Microtime200

Picoquant Microtime200

The Microtime200, manufactured by the German-based PicoQuant GmbH, is generally used in conjunction with the TCS SP5 microscope. By using the lasers from the TCS SP5, the Microtime200 is capable of measuring how fast proteins move within the cell (diffusion) and protein to protein interactions.

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Precision X-ray X-Rad 320 Biological Irradiator

Precision X-ray X-Rad 320 Biological Irradiator

The X-Rad 320 is a self contained biological irradiator for in vivo and in vitro applications.

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Raman

Raman spectroscopy

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Renishaw inVia 2 Raman Microscope (532, 633, 785 nm)

Renishaw inVia 2 Raman Microscope (532, 633, 785 nm)

The inVia 2 is a Raman spectrometer coupled to a microscope. The microscope focuses a laser source onto specific areas of a sample then collects the light scattered off the surface of the sample and directs it through a Raman spectrometer.

Capabilities of the inVia 2 Raman Microscope:

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Renishaw inVia Raman Microscope (325, 442 and 514 nm)

Renishaw inVia Raman Microscope (325, 442 and 514 nm)

The inVia is a Raman spectrometer coupled to a microscope. The microscope focuses a laser source onto specific areas of a sample then collects the light scattered off the surface of the sample and directs it through a Raman spectrometer.

Capabilities of the inVia Raman Microscope:

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SC-XRD Kappa Apex (Bruker) Single Crystal Diffractometer

The Bruker Kappa Apex is fitted with an Incoatec molybdenum X-ray microfocus source to determine the molecular and crystal structure of materials from a single crystal. The instruments is usually operated on a service-basis by an experienced crystallogrpahy, although user training is encouraged.

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Shandon Exceisior ES Tissue Processor

Shandon Exceisior ES Tissue Processor

Shandon Excelsior ES Tissue Processor is used for automated fixation, dehydration, clearing and wax infiltration of specimens prior to paraffin embedding and sectioning. Subsequent processing like staining and immunostaining can then be performed on processed specimen for pathology diagnosis or research. You can process up to 222 samples at once, across 3 level of organised baskets. The samples are hold into specimen cassettes and each level of organised basket can contain up to 74 cassettes.

 

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Shimadzu HPLC

The Shimadzu Prominence HPLC system is used for both analytical and semi-preparative separations. The instrument is equipped with fluorescence, photo diode array (PDA), and evaporative light scattering (ELSD) detectors, and is combined with a fraction collector. The instrument has a multitude of set-ups and combination and is capable of delivering both isocratic and gradient solvent elution from 0.2 to 10.5 mL/minute.

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Shimadzu UFLCxr

The Shimadzu XR Prominence HPLC system is used for analytical separations. The instrument has a binary pump set-up to allow both isocratic and gradient elution at high back pressures (circa 14 000 Psi), and is equipped with a photo diode array detector (PDA). A wide range of separations are performed on the instrument encompassing both normal and reverse phase conditions, with a wide variety of column chemistries utilised for identification and quantitative analysis.

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Smartlab (Rigaku) 9kW rotating anode thin-film XRD

High intensity (9kW) rotating copper anode X-ray source for rapid analysis of thin films.

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SPEX Freezer/Mill 6970D

SPEX Freezer/Mill 6970D

Milling of solid materials under cryogenic conditions

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Stylus Profiler

The Alpha-Step D-600 provides rapid, high resolution 2D and 3D profiling of a sample surface with a vertical range of 1200 micrometers and sub-Angstrom resolution. The instrument has a high resolution camera for visualising the sample, and a 200 mm motorised stage with a range of motion of 150 x 178 mm. The data is particularly useful for calibrating depth profiles performed on the XPS or TOFSIMS.

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