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TA Instruments NanoDSC

This capillary Differential Scanning Calorimeter can be used for investigating the stability of proteins, nucleic acids, lipids and other macromolecules. Differential Scanning Calorimetry (DSC) is the only method that directly measures the stability of macromolecules in solution. The information obtained provides valuable insights into the factors (e.g. hydrophobic interactions, hydrogen bonding, conformational entropy and physical environment) that stabilize these biomolecules alone or as complexes, such as protein-ligand or micellar complexes.

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Thermo ESCALAB250Xi X-ray photoelectron spectrometer (XPS)

Thermo ESCALAB250Xi X-ray photoelectron spectrometer (XPS) with depth profile and elemental imaging capability.

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Thermo Focus DSQ

The Thermo DSQ II GC-MS instruments are our most up-to-date instruments for analysis of compounds that are sufficiently volatile to enter the gas phase intact. The Focus DSQ II GC-MS instrument at our Lower Campus mass spectrometry facility is configured as an electron impact (EI+) only system and is dedicated to analysis of gaseous environmental samples and for trace level analysis of underivatised volatile organic molecules in solution.

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Thermo LTQ FT

Thermo Scientific LTQ FT Ultra combines the most advanced Ion Trap and Fourier Transform Ion Cyclotron Resonance (ICR) technologies into a single instrument. The ICR cell design produces enhanced sensitivity and greater dynamic range. It is an ultra high resolution instrument for LC/MS with unprecedented analytical power and versatility. The instrument is provided with ESI, nano ESI and APCI ionization sources and CID and ECD fragmentation technologies.

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Thermo LTQ Orbitrap Velos

Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Orbitrap Velos is a hybrid instrument that incorporates both Orbitrap and linear ion trap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments.

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Thermo LTQ Orbitrap Velos ETD

Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Orbitrap Velos ETD is a hybrid instrument that incorporates both Orbitrap and linear ion trap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument. This platform allows the benefits of Orbitrap mass analysis to be exploited in highly sensitive experiments.

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Thermo LTQ Orbitrap XL

The Orbitrap Velos XL is based at lower campus and is used for non-biological samples. Orbitrap mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The Thermo Scientific LTQ Orbitrap XL is a hybrid FTMS (Fourier Transform Mass Spectrometer) that incorporates the sensitive LTQ linear ion trap and Orbitrap mass analysers.

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Thermo MAT 253 IRMS with KIEL

The Thermo Mat 253 isotope ratio mass spectrometer with Kiel IV carbonate preparation device produces carbon and oxygen isotope (δ13C and δ18O) results from CO2.  We can analyse small carbonate and apatite samples (minimum: 30 micrograms).  All samples are analysed using two primary NBS standards and an in-house secondary standard.  Materials that are routinely analysed include, predominantly, palaeoenvironmental sample like speleothem calcite, limestone, marble, dolomitic limestone, sedimentary carbonates, bone apatite, and coral.

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Thermo Orbitrap Fusion Lumos

Thermo Orbitrap Fusion Lumos

The Orbitrap Fusion Lumos includes an exclusive Tribrid design incorporating 3 different mass analysers (a quadrupole, an ion trap and an Orbitrap) enabling a large number of different experiments to be performed with a single mass spectrometer.  Software developments have also simplified the set-up of complex experiments.  Other significant technological advances including a brighter ion source and improved vacuum/pumping.  Additional enhancements of the Lumos include: greater scan speed, high mass resolving power, sensitivity and an improved ETD capability and EThcD fragm

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Thermo QExactive Plus

QExactive mass analysers are recognised for their ability to produce ultra high mass accuracy and resolution measurements in both MS and MS/MS experiments; these performance capabilities allow mass spectrometry results to be interpreted with a significant degree of confidence. The QE+ is a hybrid instrument that incorporates both quadrupole and Orbitrap mass analysers; both 1D and 2D nano liquid chromatography (LC) separations can be interfaced to this instrument.

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Thermo Scientific CryoStar NX70

Thermo Scientific CryoStar NX70

Thermo Scientific CryoStar NX70 is a high-performance cryostat designed to prepare tissue

sections of frozen samples in medical and research laboratories. Specimen temperature control range is from -50oC to +10oC and blade temperature control range is from -35oC to -5oC.  Section thickness can be ranged from 0.5 µm to 500 µm. Fresh or fixed frozen specimens, embedded in OCT, are suitable for cryostat sectioning.  Frozen sections could be used for varying staining, such as histology stains, Immunohistochemistry (IFC) and Immunofluorescence (IF) stains.

 

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Thermo Trace DSQ

The Thermo DSQ II GC-MS instruments are our most up-to-date instruments for analysis of compounds that are sufficiently volatile to enter the gas phase intact. The Mass Spectrometer of the Trace DSQ II GC-MS instrument at our Upper Campus facility can be operated in either electron impact (EI+) positive ionization mode, positive ion chemical ionization (CI+) mode, or electron capture negative ionization mode (ECNI or CI-). The Quadrupole MS gives nominal m/z assignments, accurate to +/- 0.2 Da.

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Thermo TSQ Access

The TSQ Quantum is a high performance triple stage quadrupole mass spectrometer. It includes a syringe pump, a divert/inject valve, an atmospheric pressure ionisation (API) source, and the Xcalibur™ data system. You can operate the mass spectrometer in either electrospray ionisation (ESI) mode or atmospheric pressure chemical ionisation (APCI) mode. Typically, polar compounds such as amines, peptides, and proteins are best analysed by ESI, and non-polar compounds such as steroids are best analysed by APCI.

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Thermo TSQ XLS

The TSQ XLS is a triple quadrpole mass spectrometer and it is equipped with gas chromatographic sample separation.  The instrument is capable of Electron Impact and Chemical Ionization modes and can operate in either positive and negative ion polarities.  The instrument is used predominately for small molecule quantitiave analysis.

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Thin Film Analyser - Mikropack NanoCalc 2000 UV-Vis-NIR

The instrument is a UV-visible-NIR thin film analyser which can provide a measurement of the thickness of multilayer films. The instrument measures light reflected perpendicular to a sample surface which can provide information on the thickness of up to 4 layers in a sample, and is useful for an independent comparison with film-thickness measurements obtained on the MRD. The wavelengths available range from 250nm to 850nm, which enable measurement of film thickness from 10nm up to 20micron. This instrument is located in lab G65, and it available at no charge to UNSW users.

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TILL Photonics iMic TIRF System

TILL Photonics iMic TIRF System

This microscope has a unique design, allowing for a great deal of customization of the sample space and experimental design. The system is designed for up to four-color total internal fluorescence (TIRF), photoactivation (PA), fluorescence resonance energy transfer (FRET), widefield correlation spectroscopy (WCS), spatio-temporal image correlation spectroscopy (STICS), and fluorescence recovery after photobleaching (FRAP) experiments, all at high frame rates. Single-molecule measurements can be enhanced with a pair of single-photon avalanche photodiodes (APDs) and a pulse correlator for fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) experiments. An environmental chamber allows for these techniques to be extended to live cell samples.

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Time of Flight Secondary Ion Mass Spectrometer (TOFSIMS)

The ION-TOF TOF.SIMS 5  was installed in to our lab in May 2015. The instrument is located in F10:G61. Contact Dr Bill Gong (b.gong@unsw.edu.au) for more details on its capabilities.

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VisualSonics Vevo 2100 Ultrasound

VisualSonics Vevo 2100 Ultrasound

The VisualSonics Vevo2100 uses the new solid-state transducers which offer higher resolution at multiple focal depths. The system offers superior resolution down to 30μm, a wide range of frequencies (13-56 MHz) and a large field of view. Importantly, the Vevo2100 offers a comprehensive imaging and processing packing.

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Waters/Micromass Q-TOF Ultima

The Waters Q-TOF Ultima is a quadrupole, orthogonal acceleration time-of-flight tandem mass spectrometer. It equipped with ESI and nanoESI interfaces. It has high resolution, sensitivity and mass accuracy. The Q-TOF Ultima is mainly used for LC-coupled MSMS analysis of peptides derived from gel spots and bands, accurate mass determination of biomolecules, protein post-translational modification studies, e.g. glycopeptide mapping.

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Wavelength dispersive X-ray fluorescence spectrometers

The AXIOS 4kW rhodium source wavelength dispersive X-ray fluorescence (WD-XRF) spectrometer is a LIEF-funded instrument installed in 2009 to complement our older PW2400 3kW rhodium source WD-XRF. The instrument can analyse elements from Na to U, as well as B, Cl and F. Our major element suite includes the following, plus loss on ignition (L.O.I.): Na, Mg, Al, Si, P, S, K, Ca, Ti, Mn and Fe. For trace elements please contact the XRF laboratory, as we have a range of different packages for various trace element combinations. Our B program is specifically setup to analyse glasses.

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XRD Experiments Using Variable Temperature or Controlled Atmophere

The diffraction instruments have a range of accessories for dynamic monitoring of phase changes from 10-1800 Kelvin, in certain cases using a controlled atmosphere.

Bruker D8 with rotating anode copper source:

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Zeiss Axio Observer X.1 SD & TIRF

Zeiss Axio Observer X.1 SD & TIRF

The zeiss Spinning Disk/TIRF system is a custom built instrument.  Built upon an Axio Observer X.1 body it is equipped with a Yokogawa Spinning disk CSU-X1 module providing confocal imaging at rapid frame rates captured on an EM CCD camera (Quant EM) ideally suited for low light imaging. Attached to a different microscope port is a highly sensitive high frame rate sCMOS camera (Orca Flash 4) which can be used for standard epifluorescence, rapid ratiometric imaging utilising a fast excitation switching light source (Lambda DG4) or acquire fluorescence images using TIRF illumination.

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