This microscope has a unique design, allowing for a great deal of customization of the sample space and experimental design. The system is designed for up to four-color total internal fluorescence (TIRF), photoactivation (PA), fluorescence resonance energy transfer (FRET), widefield correlation spectroscopy (WCS), spatio-temporal image correlation spectroscopy (STICS), and fluorescence recovery after photobleaching (FRAP) experiments, all at high frame rates. Single-molecule measurements can be enhanced with a pair of single-photon avalanche photodiodes (APDs) and a pulse correlator for fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) experiments. An environmental chamber allows for these techniques to be extended to live cell samples.
The VisualSonics Vevo3100 uses the new solid-state transducers which offer higher resolution at multiple focal depths. The system offers superior resolution down to 30μm, a wide range of frequencies (13-56 MHz) and a large field of view. Importantly, the Vevo3100 offers a comprehensive imaging and processing packing.
The zeiss Spinning Disk/TIRF system is a custom built instrument. Built upon an Axio Observer X.1 body it is equipped with a Yokogawa Spinning disk CSU-X1 module providing confocal imaging at rapid frame rates captured on an EM CCD camera (Quant EM) ideally suited for low light imaging. Attached to a different microscope port is a highly sensitive high frame rate sCMOS camera (Orca Flash 4) which can be used for standard epifluorescence, rapid ratiometric imaging utilising a fast excitation switching light source (Lambda DG4) or acquire fluorescence images using TIRF illumination.
The Zeiss Elyra Superresolution Microscope is a developmental superresolution microscope with capabilities to push imaging past the classic diffraction limit. Typically visible light microscopes cannot achieve spatial resolution of greater than 200-250 nanometers. This system has two different imaging modalities to push this limit as low as 20 nanometers.
The Zeiss LSM 800 is an inverted Zeiss Axio Observer Z1 laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector, 2 multi-alkali PMTs and DIC optics for trans-illumination microscopy with each objective. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (120 nm in x / y and 350 nm in z) and 4-8 x better signal-to-noise ratio, with fast linear scanning. Airyscan works with thicker samples such as fixed tissue sections or whole animal mounts that need a higher penetration depth.
The Zeiss LSM 880 is an inverted laser scanning confocal microscope equipped with high quantum efficiency GaAsP detector and 2 multialkali PMTs, which can be used as single point detectors or combined for 34 channel spectral imaging. In addition, the Airyscan module can deliver images with 1.7 x improved resolution (140nm axially, 400 nm laterally) and 4-8 x greater signal-to-noise ratio, at the highest speed of any linear scanning confocal system. BiG.2 Type A (2x GaAsP detectors) non-descanned detectors for the multi-photon laser can be used for imaging thick specimens, with deeper penetration and decreased phototoxicity compared to single photon lasers. The Picoquant system utilises the multi-photon laser to perform Fluorescence Lifetime Imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Forster Resonance Energy Transfer (FRET). The Zeiss 880 is also equipped with polarisers for anisotropy measurements.