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Lavision TriM Scope II Multiphoton Intravital

Lavision TriM Scope II Multiphoton Intravital

This intravital microscope boasts an impressive collection of hardware. Equipped with 2 multiphoton lasers utilising a pre-chirp compensator to keep laser pulse width < 150 fs, a collection of detectors including high sensitive NDD detectors close to the objective lens, descanned PMTs, imaging cameras for fast 64 beam scanning and also LaVision BioTec's FLIM x16 TCSPC detector for rappid fluorescence lifetime measurements. 

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Leica 165C Microscope

The Leica microscope has a large working distance between the objective and sample and an incident cold light source to enable our crystallographers to mount single-crystals onto a capillary, prior to measuring the diffraction pattern. Images and dimensions of a crystal can be collected using the CCD camera. When not in use by our crystallographers, the microscope may be used, at no cost, by UNSW researchers who require a low to medium magnification (max.90x) digital image of their sample.

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Leica DM IL

Leica DM IL

The Leica DM IL microscope is used to illuminate whole cells; observing and analysing a cell as a single entity. The DM IL is an inverted epifluorescence microscope, with transmitted light LED back illumination and an EL6000 Fluoro system.

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Leica RM2135 Rotary Microtome

Leica RM2135 Rotary Microtome

Leica RM2135 rotary microtome is used to cut very thin cross-sections of specimens (usually 4 µm) for routine and research histology studies in the fields of biology and medicine.  This instrument is designed for cutting soft and harder paraffin embedded specimens, as long as they are suitable for being cut manually.  The sectioning thickness setting range can be from 1 µm to 60 µm.  High quality paraffin sections could be used for varying staining, such as Haematoxylin and Eosin stain (H&E), immunohistochemistry (IHC), Immunofluorescent staining (IF) and In situ hybridization (ISH).

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Leica SP8 DLS

Leica SP8 DLS

The Leica TCS SP8 DLS (Digital LightSheet) is an inverted laser scanning microscope capable to be used for confocal imaging and light sheet imaging. This microscope is able to image multi-colour, live, fixed or aqueous-based cleared specimens utilising a combinations of 405, 458, 488, 514, 561 and 635 nm excitation lasers through the employment of the AOBS with spectral high quantum efficiency detectors. The advantage of the two combined features (confocal/Lightsheet) on a single equipment allow to perform, with the confocal module, local photo activation or photo bleaching where the resulting dynamics can be capture via lightsheet imaging on thick sample (up to 2 mm thick and 1 cm long).

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Leica TCS SP5 MP STED

Leica TCS SP5 MP STED

Leica TCS SP5 equipped with a Spectra-Physics Mai Tai Ti:Sapphire laser is an inverted confocal laser scanning microscope. It is designed for high-resolution fluorescence and transmitted light imaging with single or multi-photon excitation, in 2D, 3D or 4D with live or fixed cells. It can also be used for Fluorescence Recovery After Photo bleaching (FRAP), pulsed Stimulated Emission Depletion (pulsed STED) and Fluorescence Correlation Spectroscopy (FCS). In combination with the Picoquant Microtime200, it can be used for Fluorescence Correlation Spectroscopy (FCS) coupled to Fluorescence Lifetime Imaging (FLIM).

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Leica TCS SP5 WLL STED

Leica TCS SP5 WLL STED

This Leica TCS SP5 confocal system is equipped for a broad range of experimental requirements. Along with a range of fixed wavelength laser lines it now has a White-Light Laser (WLL) tunable within the range x-x for precise control over fluorophore excitation. Fitted with Hybrid Dectors (HyD) for enhanced sensitivity, and interchangeable Galvano and resonant scanners, this system is suitable for applications requiring both speed and/or improved signal-to-noise. Its Gated-STED module also enables colleciton of super-resolution images allowing investigators to distinguish objects and structures beyond the conventional resolution limit of light.

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Liquid Chromatography

Separation and Fractionation

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Liquid Chromatography / Mass Spectrometry

Proteins, Polymers and other High MW or Polar Molecules including Proteomics

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Matrix Assisted Laser Desorption / Ionisation

Proteins, Polymers and other High MW or Polar Molecules including Proteomics

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MPD (PANalytical) Xpert Multipurpose X-ray Diffraction System

The MPD is a Bragg-Brentano geometry X-ray diffractometer suitable for routine measurement of powder samples, either phase identification or quantitative analysis. The instrument is equipped with the following components:

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MRD (PANalytical) Xpert Materials Research diffractometer

The MRD is a highly flexible X-ray diffraction system, equipped with X-ray mirror, (220) Ge monochromator, X-ray lens, mono-capillary, triple-axis goniometer, and is used for analysis of thin films, nanostructure, semiconductor materials, stresses and textures. It is equipped with a few active components, such as an X-ray mirror, Ge 220 monochromator, X-ray lens and mono-capillary for incident beam conditioning, and parallel plate collimator or triple-axis for diffracted beam conditioning. Both proportional and area detectors are available.

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Multispectral Optoacoustic Tomography

Multispectral Optoacoustic Tomography

Optoacoustic or photoacoustic imaging is a new, cutting-edge imaging technique which detects ultrasound waves following light absorption from specific molecules.  This technique allows molecules with different spectral profile to be detected.

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Muromachi Microwave Fixation System

Muromachi Microwave Fixation System

The Muromachi microwave fixation system offers an effective way to inactivate these enzymes in the intact specimen.

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NanoSight NS300

NanoSight NS300

The NanoSight NS300 utilizes the latest version of NanoSight’s particle characterization technology, Nanoparticle Tracking Analysis (NTA). This elegant new instrument offers the unique benefits scientists have come to expect from NanoSight in a compact format, with the major benefit of enhanced fluorescence capability.

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Nikon A1 Intravital

Nikon A1 Intravital

The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability. 

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Nikon Biostation

Nikon Biostation

Nikon Biostation is an inverted life cell imaging microscope enclosed in an incubator. The stage and objective are fully enclosed in temperature modulated box resulting in no focus drift during imaging. The cells are kept at 37C and supplemented with 5% CO2 throughout the entire experiment. The imaging chamber can hold 35mm glass or plastic bottom dishes of 0.17um thickness. The Biostation is an ideal microscope for imaging cell migrations, wound healing, cell division and cell growth and adherence.

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Nikon Eclipse Tie

Nikon Eclipse Tie

The Nikon Eclipse TiE is ideally suited for live cell imaging studies. With one of the best focus stabilisation devices on the market the Perfect Focus System (PFS) ensures that the focus you set at the start of the experiment will be maintained throughout its entirety. They system is fitted with several phase contrast objects making it suitable for imaging cells through a range of materials including plastic, as well as 3 high NA DIC objects for achieving astounding detail if imaging through glass.

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Nikon TIRF/HiLo

This microscope combines a total internal fluorescence (TIRF) and highly inclined and laminated optical sheet (HiLo: https://www.nature.com/articles/nmeth1171) modalities in one instrument. The TIRF modality allows user to image molecules at surface of cells and apply super- resolution imaging techniques such as PALM and STORM. HiLo modality offers the possibility of exciting a thin inclined plane within cells or thin layer of cells within few micrometers of a live animal tissue. Automatized variability of angle of HiLo emergence and camera imaging allows for fast sectioning of sample. The system is also supplemented with NIDAQ board and control with external trigger, which combined with the versatile illumination sequence option makes this ideal microscope for complex experiments involving the correct timing of imaging following an external stimulus of cells or animal. Definite focus is perfectly controlled using Nikon PFS system making it ideal for long term live cell or animal imaging. 

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NuSep ProteomSep/MF10 Electrophoretic Partitioning Systems

This in-solution electrophoretic device is for the partitioning of μl volumes of μg-mg amounts of proteins and or peptides based on surface charge, pH, size in denaturing or native formats. It is typically used to partition contaminant proteins away from lower abundance proteins (eg. Albumin or Ig from other plasma proteins), desalting of biological samples (eg. urine, plasma, tears), enrichment of poorly concentrated and low abundance samples for further analysis. Partitioning down to 1kDa is achievable and is therefore ideal for peptide and small protein work.

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Off-line Separation

1 dimensional & 2 dimensional gel eletrophoresis (SDS PAGE & 2DE) and in-solution electrophoretic partitioning are important off-line separation methods for large biomolecules such as proteins and nucleic acids. The BMSF has a comprehensive range of separation devices and mass spectrometers to address most analytical problems suited to mass spectrometry.

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Olympus Delta Premium Portable XRF Spectrometer

The Olympus Delta Premium PXRF Spectrometer was purchased in 2014. This is normally a service only instrument, but user training is possible if you contact the lab. The instrument comes with two factory calibrations (Geochem and Soil), as well as calibrations for coal, and can be calibrated to suit a specific sample type.

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Optical Microscope

HIgh-resolution optical microscope with camera with 5x, 20x, 50x and 100x objectives.

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PANalytical AXIOS Advanced Simultaneous WDXRF Spectrometer

The PANalytical AXIOS-Advanced WDXRF spectrometer was commissioned in 2010 and is fitted with an SST-MAX X-ray sourcewith a 4kW output and can be operated at 160mA. The resulting high X-ray flux enables high sensitivity and low detection limits for light elements. The AXIOS can measure elements over wide dynamic concentration ranges and include all the elements between Be and U in the periodic table.

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